Construction and function analysis of luciferase reporter plasmid with myocardin gene promoter

2011 ◽  
Author(s):  
Meng-Meng Qiang ◽  
Nan Wang ◽  
Xiang-Zheng Hu ◽  
Tong-Cun Zhang
Keyword(s):  
Function Analysis ◽  
Gene Promoter ◽  
Luciferase Reporter ◽  
Reporter Plasmid ◽  
Luciferase Reporter Plasmid ◽  
And Function

Construction of Luciferase Reporter Plasmid with Human AQP1 Gene Promoter

2012 ◽  
Vol 424-425 ◽  
pp. 399-402
Author(s):  
Zhe Sun ◽  
Tong Cun Zhang ◽  
Yong Jiang
Keyword(s):  
Water Channel ◽  
Gene Promoter ◽  
Luciferase Reporter ◽  
Reporter Plasmid ◽  
Luciferase Reporter Gene ◽  
Expression Plasmid ◽  
Luciferase Reporter Plasmid ◽  
Osmotic Water ◽  
And Function ◽  
Transcription Expression

Aquaporins (AQPs), a family of water channels, function as a water-selective transporting protein in cell membranes. Aquaporin 1 (AQP1) was first discovered in human erythrocytes as a water channel for high osmotic water permeability. AQP1 promoter is responsible for regulation of its transcription and expression. In this study, molecular biology and genetic engineering knowledge and principles of construction of AQP1 promoter plasmid, and recombinant plasmids were identified and measured for luciferase, for promoter function. Our study successfully constructed AQP1 promoter luciferase reporter gene expression plasmid and successfully carry out the functions of its detection, provides a new foundation for the transcription expression and function regulation of the AQP1

Function Analysis of Luciferase Reporter Plasmid with AQP1 Gene Promoter

2011 ◽  
Vol 396-398 ◽  
pp. 1486-1489
Author(s):  
Yong Jiang ◽  
Zhe Sun ◽  
Tong Cun Zhang
Keyword(s):  
Transcriptional Regulation ◽  
Function Analysis ◽  
Gene Promoter ◽  
Luciferase Reporter ◽  
Reporter Plasmid ◽  
Reporter Construct ◽  
Luciferase Reporter Construct ◽  
Transcription Activity ◽  
Luciferase Reporter Plasmid ◽  
Precise Role

The aquaporins (AQP) are a family of homologous water transporting proteins that are expressed in many epithelial, endothelial and other tissues. Myocardin is important for SMC differentiation, but its precise role in regulating the initiation of AQP1 transcription activity is less clear. Function analysis of AQP1 promoter luciferase reporter plasmid will provide the theory basis for researching the function of transcription activity. In this study, the rat and human myocardin promoter luciferase reporter construct were successfully constructed. Then to determine whether AQP1 transcription activity were regulated by myocardin, luciferase repoeter assays were performed in COS-7 cells. The results illustrated that myocardin significantly activated rat and human AQP1 promoter. AQP1 promoter transactivity was inhibited by △Q. The present study provided the first evidence that myocardin may have an influence on the expression of AQP1 and reveal a basis of the mechanism transcriptional regulation of AQP1.

scholarly journals Construction and functional analysis of luciferase reporter plasmid containing connexin43 gene promoter

2017 ◽  
Vol 8 ◽  
pp. 01007
Author(s):  
Hui Li ◽  
Jiapeng Li ◽  
Weilin Shi ◽  
Xiaoyu Zhang ◽  
Yuan Xiang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Gene Promoter ◽  
Luciferase Reporter ◽  
Reporter Plasmid ◽  
Luciferase Reporter Plasmid

Construction and Functional Analysis of Luciferase Reporter Plasmid Containing NF-H Gene Promoter

2014 ◽  
Vol 915-916 ◽  
pp. 942-946
Author(s):  
Tong Cun Zhang ◽  
Yao Meng ◽  
Nan Wang ◽  
Feng Lin ◽  
Tao Qin ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gene Promoter ◽  
Luciferase Reporter ◽  
Reporter Plasmid ◽  
Extracellular Signal Regulated Kinase ◽  
Luciferase Reporter Plasmid ◽  
Extracellular Signal ◽  
Mature Neurons ◽  
Reporter Assays ◽  
Transcription 3

NF-H (a member of neurofilaments) is a protein widely expressed in all kinds of neurons after birth and can be used as one of the symbols of mature neurons. Constuction of NF-H promoter luciferase reporter plasmid will provide the theory basis for researching the effect of other transcription factors on regulating NF-H transcription. Here, human NF-H promoter luciferase reporter plasmid were successfully constructed. Then the effects of some key transcription factors were investigated by luciferase reporter assays in COS-7 cells. The results showed that retinoid X receptor α (RXRα) can enhance transcriptional activity of NF-H. Furthermore, ERK 1/2 (extracellular signal-regulated kinase 1/2) and STAT3 (signal transducer and activator of transcription 3) also show obvious impact in activating NF-H transcription.

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