scholarly journals Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion

2009 ◽  
Vol 13 (12) ◽  
pp. 3274-3284 ◽  
Author(s):  
Kimberly Trabbic-Carlson ◽  
Li Liu ◽  
Bumjoon Kim ◽  
Ashutosh Chilkoti
Keyword(s):  
Escherichia Coli ◽  
Recombinant Proteins ◽  
Expression And Purification

scholarly journals High Level Expression and Purification of Recombinant Proteins from Escherichia coli with AK-TAG

PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0156106 ◽  
Author(s):  
Dan Luo ◽  
Caixia Wen ◽  
Rongchuan Zhao ◽  
Xinyu Liu ◽  
Xinxin Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Proteins ◽  
Expression And Purification ◽  
High Level Expression ◽  
High Level

scholarly journals Expression, Purification, and Functional Analysis of Novel RelE Operon fromX. nematophila

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Jitendra Singh Rathore ◽  
Lalit Kumar Gautam
Keyword(s):  
Escherichia Coli ◽  
Functional Analysis ◽  
Recombinant Proteins ◽  
Bacterial Toxin ◽  
Type Ii ◽  
Xenorhabdus Nematophila ◽  
Expression And Purification ◽  
Toxicity Assay ◽  
Neutralization Activity ◽  
E Coli

Bacterial toxin-antitoxin (TA) complexes induce programmed cell death and also function to relieve cell from stress by various response mechanisms.Escherichia coliRelB-RelE TA complex consists of a RelE toxin functionally counteracted by RelB antitoxin. In the present study, a novel homolog of RelE toxin designated as Xn-relE toxin fromXenorhabdus nematophilapossessing its own antitoxin designated as Xn-relEAT has been identified. Expression and purification of recombinant proteins under native conditions with GST and Ni-NTA chromatography prove the existence of novel TA module. The expression of recombinant Xn-relE under tightly regulated ara promoter inE. coliTop 10 cells confirms its toxic nature in endogenous toxicity assay. The neutralization activity in endogenous toxicity assay by Xn-relEAT antitoxin confirms its antidote nature when studying the whole TA operon under ara regulated promoter. This study promotes newly discovered TA module to be regarded as important as other proteins of type II toxin-antitoxin system.

Expression and purification of recombinant proteins in Escherichia coli tagged with the metal-binding protein CusF

2016 ◽  
Vol 121 ◽  
pp. 61-65 ◽  
Author(s):  
J. Enrique Cantu-Bustos ◽  
Teresa Vargas-Cortez ◽  
Jose Ruben Morones-Ramirez ◽  
Isaias Balderas-Renteria ◽  
David W. Galbraith ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Proteins ◽  
Metal Binding ◽  
Binding Protein ◽  
Expression And Purification ◽  
Metal Binding Protein

Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+

2020 ◽  
pp. 329-344
Author(s):  
Jessica J. Gomez-Lugo ◽  
Bryan D. Santos ◽  
David A. Perez-Perez ◽  
Jorge M. Montfort-Gardeazabal ◽  
Megan M. McEvoy ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Proteins ◽  
Metal Binding ◽  
Binding Proteins ◽  
Expression And Purification

Expression and Purification of Bioactive High-Purity Recombinant Mouse SPP1 in Escherichia coli

2014 ◽  
Vol 173 (2) ◽  
pp. 421-432 ◽  
Author(s):  
Yunsheng Yuan ◽  
Xiyuan Zhang ◽  
Shunyan Weng ◽  
Wen Guan ◽  
Di Xiang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Purity ◽  
Expression And Purification ◽  
Recombinant Mouse
Sign in / Sign up

Export Citation Format

Share Document