Molecular identification of fungi in formalin-fixed and paraffin-embedded skin tissue samples

2018 ◽  
Vol 46 (2) ◽  
pp. 171-172
Author(s):  
Taketoshi Futatsuya ◽  
Kazushi Anzawa ◽  
Takashi Mochizuki ◽  
Yutaka Hatano
Keyword(s):  
Molecular Identification ◽  
Skin Tissue ◽  
Tissue Samples ◽  
Identification Of Fungi ◽  
Formalin Fixed

scholarly journals The Dutch National TissueArchive Portal enables efficient, consistent, and transparent procurement of diagnostic tissue samples for scientific use

2021 ◽  
Author(s):  
Robin Verjans ◽  
Annette H. Bruggink ◽  
Robby Kibbelaar ◽  
Jos Bart ◽  
Aletta Debernardi ◽  
...  
Keyword(s):  
The Netherlands ◽  
Tissue Sample ◽  
Ffpe Tissue ◽  
Tissue Samples ◽  
Internet Portal ◽  
Formalin Fixed Paraffin ◽  
Practical Support ◽  
Formalin Fixed Paraffin Embedded ◽  
The Rich ◽  
Formalin Fixed

AbstractBiobanks play a crucial role in enabling biomedical research by facilitating scientific use of valuable human biomaterials. The PALGA foundation—a nationwide network and registry of histo- and cytopathology in the Netherlands—was established to promote the provision of data within and between pathology departments, and to make the resulting knowledge available for healthcare. Apart from the pathology data, we aimed to utilize PALGA’s nationwide network to find and access the rich wealth of Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples for scientific use.  We implemented the Dutch National TissueArchive Portal (DNTP) to utilize PALGA’s nationwide network for requesting FFPE tissue samples. The DNTP consists of (1) a centrally organized internet portal to improve the assessing, processing, harmonization, and monitoring of the procurement process, while (2) dedicated HUB-employees provide practical support at peripheral pathology departments. Since incorporation of the DNTP, both the number of filed requests for FFPE tissue samples and the amount of HUB-mediated support increased 55 and 29% respectively. In line, the sample procurement duration time decreased significantly (− 47%). These findings indicate that implementation of the DNTP improved the frequency, efficiency, and transparency of FFPE tissue sample procurement for research in the Netherlands. To conclude, the need for biological resources is growing persistently to enable precision medicine. Here, we access PALGA’s national, pathology network by implementation of the DNTP to allow for efficient, consistent, and transparent exchange of FFPE tissue samples for research across the Netherlands.

Microsatellite instability evaluation: which test to use for endometrial cancer?

2021 ◽  
pp. jclinpath-2021-207723
Author(s):  
Paola Rafaniello-Raviele ◽  
Ilaria Betella ◽  
Alessandra Rappa ◽  
Davide Vacirca ◽  
Gianluca Tolva ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Microsatellite Instability ◽  
Automated System ◽  
Tissue Samples ◽  
Valuable Alternative ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed ◽  
Mmr Proteins

AimsAnalysis of microsatellite instability (MSI) is strongly recommended in endometrial cancer (EC) and colorectal cancer to screen for Lynch syndrome, to predict prognosis and to determine optimal treatment and follow-up. In a large monoinstitutional series of ECs, we evaluated the reliability and accuracy of Idylla assay, a rapid, fully automated system to detect MSI, and we compared its performance with two routine reference methods.MethodsWe evaluated MSI status in 174 formalin-fixed, paraffin-embedded EC tissue samples using immunohistochemistry (IHC) for mismatch repair (MMR) proteins and Idylla assay. Samples with discordant or equivocal results were analysed with a third technique, the Promega MSI kit.ResultsIdylla MSI assay and IHC were highly concordant (overall agreement: 154/170=90.59%, 95% CI 85.26% to 94.12%). However, in four samples, MMR-IHC staining was equivocal; moreover, 16 cases showed discordant results, that is, MMR deficient using IHC and microsatellite stable using Idylla. These 20 samples were reanalysed using the MSI-Promega kit, which showed the same results of Idylla assay in 18/20 cases (overall agreement: 90%, 95% CI 69.90% to 97.21%).ConclusionsOur results suggest that IHC is an efficient method to determine MMR status in ECs. However, the Idylla MSI assay is a rapid and reliable tool to define MSI status, and it could represent a valuable alternative to conventional MSI-PCR methods.

scholarly journals Isolation and molecular Identification of fungi associated with the spoilage of farms fruit in Southern Cte dIvoire

2014 ◽  
Vol 8 (34) ◽  
pp. 3171-3177
Author(s):  
Kamel Kossonou Yao ◽  
Clementine Kouakou Amenan ◽  
Daniel Kra Kouam ◽  
H. Tra Bi Fezan ◽  
A. Diallo Hortense ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Identification Of Fungi

scholarly journals Preliminary Comparison of Quantity, Quality, and Microarray Performance of RNA Extracted From Formalin-fixed, Paraffin-embedded, and Unfixed Frozen Tissue Samples

2006 ◽  
Vol 54 (11) ◽  
pp. 1229-1237 ◽  
Author(s):  
Marshall S. Scicchitano ◽  
Deidre A. Dalmas ◽  
Melissa A. Bertiaux ◽  
Shawn M. Anderson ◽  
Leah R. Turner ◽  
...  
Keyword(s):  
Tissue Samples ◽  
Preliminary Comparison ◽  
Formalin Fixed Paraffin ◽  
Frozen Tissue ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

The UNITE database for molecular identification of fungi - recent updates and future perspectives

2010 ◽  
Vol 186 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Kessy Abarenkov ◽  
R. Henrik Nilsson ◽  
Karl-Henrik Larsson ◽  
Ian J. Alexander ◽  
Ursula Eberhardt ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Future Perspectives ◽  
Identification Of Fungi

scholarly journals Pathogenicity Assay and Molecular Identification of Fungi and Bacteria Associated with Diseases of Tomato in Malaysia

2016 ◽  
Vol 07 (06) ◽  
pp. 949-957 ◽  
Author(s):  
Tavga Sulaiman Rashid ◽  
Kamaruzaman Sijam ◽  
Hayman Kakakhan Awla ◽  
Halimi Mohd Saud ◽  
Jugah Kadir
Keyword(s):  
Molecular Identification ◽  
Identification Of Fungi ◽  
Pathogenicity Assay

scholarly journals Evaluation of Formalin-fixed Paraffin-embedded Tissue Samples Diagnosed by Histopathology as Echinococcus in Çorum

2021 ◽  
Vol 45 (4) ◽  
pp. 262-267
Author(s):  
Yılmaz Baş ◽  
Yunus Emre Beyhan ◽  
Havva Hande Keser Şahin ◽  
Tuğba Özçerezci ◽  
Dursun Karasartova ◽  
...  
Keyword(s):  
Tissue Samples ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Isolation and molecular identification of fungi producing L-methioninase enzymes isolated from Makkah region soil

2021 ◽  
Vol 13 (1) ◽  
pp. 61-74
Author(s):  
N. H. Alzahrani ◽  
K. H. Alamoudi ◽  
A. Kh. Balobaid
Keyword(s):  
Molecular Identification ◽  
Identification Of Fungi
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