scholarly journals Rapid detection of enterobacteriaceae producing extended spectrum beta-lactamases directly from positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry

2014 ◽  
Vol 20 (11) ◽  
pp. 1146-1157 ◽  
Author(s):  
M. Oviaño ◽  
B. Fernández ◽  
A. Fernández ◽  
M.J. Barba ◽  
C. Mouriño ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Laser Desorption ◽  
Blood Cultures ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Beta Lactamases ◽  
Flight Mass Spectrometry ◽  
Extended Spectrum Beta Lactamases ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Rapid Identification of Bacteria Directly from Positive Blood Cultures by Use of a Serum Separator Tube, Smudge Plate Preparation, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

2015 ◽  
Vol 53 (10) ◽  
pp. 3349-3352 ◽  
Author(s):  
Yan Chen ◽  
Vanessa Porter ◽  
Samira Mubareka ◽  
Leona Kotowich ◽  
Andrew E. Simor
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Blood Cultures ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

We analyzed the matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) of smudge plate growth for bacterial identification from 400 blood cultures. Ninety-seven percent of Gram-negative bacilli and 85% of Gram-positive organisms were correctly identified within 4 h; only eight isolates (2.0%) were misidentified. This method provided rapid and accurate microbial identification from positive blood cultures.

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