scholarly journals Identification of Blood Culture Isolates Directly from Positive Blood Cultures by Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry and a Commercial Extraction System: Analysis of Performance, Cost, and Turnaround Time

2012 ◽  
Vol 50 (10) ◽  
pp. 3324-3328 ◽  
Author(s):  
P. R. S. Lagace-Wiens ◽  
H. J. Adam ◽  
J. A. Karlowsky ◽  
K. A. Nichol ◽  
P. F. Pang ◽  
...  
2015 ◽  
Vol 53 (10) ◽  
pp. 3349-3352 ◽  
Author(s):  
Yan Chen ◽  
Vanessa Porter ◽  
Samira Mubareka ◽  
Leona Kotowich ◽  
Andrew E. Simor

We analyzed the matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) of smudge plate growth for bacterial identification from 400 blood cultures. Ninety-seven percent of Gram-negative bacilli and 85% of Gram-positive organisms were correctly identified within 4 h; only eight isolates (2.0%) were misidentified. This method provided rapid and accurate microbial identification from positive blood cultures.


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