Cloning and characterization of Methoprene‐tolerant (Met) and Krüppel homolog 1 (Kr‐h1) genes in the wheat blossom midge, Sitodiplosis mosellana

2018 ◽  
Vol 27 (2) ◽  
pp. 292-303
Author(s):  
Wei‐Ning Cheng ◽  
Xue‐Jiao Li ◽  
Jia‐Jia Zhao ◽  
Keyan Zhu‐Salzman
Author(s):  
J. F. Doane ◽  
M. P. Braun ◽  
O. O. Olfert ◽  
F. Affolter ◽  
K. Carl

Insects ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 891
Author(s):  
Weining Cheng ◽  
Yudong Zhang ◽  
Jinlin Yu ◽  
Wei Liu ◽  
Keyan Zhu-Salzman

The wheat blossom midge Sitodiplosis mosellana, one of the most disastrous wheat pests, depends highly on olfactory cues to track suitable plants. To better understand the olfactory recognition mechanisms involved in host selection, in the present study we cloned two S. mosellana adult antenna-specific odorant binding protein (OBP) genes, SmosOBP12 and SmosOBP17, and evaluated bacterially expressed recombinant proteins for their selectivity and sensitivity for host wheat volatiles using the fluorescence-based ligand binding assay. The results showed that both SmosOBPs effectively bound alcohol, ester, ketone, and terpenoid compounds. Particularly, SmosOBP12 had significantly higher affinities (Ki < 10.5 μM) than SmosOBP17 (Ki2 > 0.1 μM) to 3-hexanol, 1-octen-3-ol, D-panthenol, 3-carene, (Z)-3-hexenylacetate, hexyl acetate, methyl salicylate, heptyl acetate, and ethyl heptanoate. Consistently, S. mosellana females were attracted to all these chemicals in a behavioral assay using Y-tube olfactometer. SmosOBP12 also bound aldehyde, but neither bound alkanes. Notably, SmosOBP12 exhibited strong affinity to ocimene (Ki = 8.2 μM) that repelled S. mosellana. SmosOBP17, however, was insensitive to this compound. Taken together, our results indicate that SmosOBP12 may play a greater role than SmosOBP17 in perceiving these biologically active plant volatiles.


2015 ◽  
Vol 95 (4) ◽  
pp. 799-803 ◽  
Author(s):  
P. D. Brown ◽  
H. S. Randhawa ◽  
J. Mitchell Fetch ◽  
S. L. Fox ◽  
D. G. Humphreys ◽  
...  

Brown, P. D., Randhawa, H. S., Mitchell Fetch, J., Fox, S. L., Humphreys, D. G., Meiklejohn, M., Green, D., Wise, I., Fetch, T., Gilbert, J., McCallum, B. and Menzies, J. 2015. AAC Foray red spring wheat. Can. J. Plant Sci. 95: 799–803. AAC Foray, an orange wheat blossom midge (Sitodiplosis mosellana Géhin) tolerant hard red spring wheat (Triticum aestivum L.), combined high grain yield and good agronomic performance with excellent resistance to leaf and stem rust, and improved resistance to Fusarium head blight. AAC Foray had maturity, straw strength, and test weight similar to the check cultivars. AAC Foray is eligible for grade of the Canada Prairie Spring Red wheat market class.


1993 ◽  
Vol 125 (4) ◽  
pp. 725-736 ◽  
Author(s):  
Kenneth A. Pivnick ◽  
Edith Labbé

AbstractThe daily patterns of activity of females of the orange wheat blossom midge, Sitodiplosis mosellana (Géhin), were observed in controlled laboratory conditions and in field conditions in eastern Saskatchewan in mid-July 1986 and 1987. In the field, during the daytime, females rested on stems of wheat plants within 30 cm of the ground and, at approximately 2000 hours CST, flew up to wheat heads. Most oviposition took place between 2000 and 2145 hours (or 75 min before, to 30 min after, sunset). Toward the end of the oviposition period, females were frequently seen drinking dew from wheat heads. On some evenings, females migrated down from the heads following oviposition, but on more than half of the evenings they remained on the heads until early morning. However, they never moved down to the low level they occupied during the day until the next morning, when the migration was usually complete by 0900–1000 hours. Light intensity appeared to regulate the vertical migration of females. Cloudy conditions may allow an earlier onset of oviposition. Flight was limited to air temperatures above 14–15 °C and oviposition to temperatures above 10–11 °C. Wind speeds of 10 km per h or more and rain occasionally limited activity. In the laboratory, oviposition activity occurred almost exclusively during the scotophase, mainly in the first 2 h. Mean total fecundity was 83.6 ± 10.9 (±SE) eggs, and mean longevity was 6.6 ± 0.6 days. No oviposition took place on the 1st night, and the greatest mean daily fecundity occurred on the 3rd night.


2009 ◽  
Vol 20 (2) ◽  
pp. 89-92 ◽  
Author(s):  
Toby J.A. Bruce ◽  
Lesley E. Smart

2015 ◽  
Vol 35 (11) ◽  
Author(s):  
武予清 WU Yuqing ◽  
段爱菊 DUAN AiJu ◽  
张自启 ZHANG ZiQi ◽  
刘长营 LIU ChangYing ◽  
刘顺通 LIU ShunTong ◽  
...  

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