Honey bee-assisted surveillance for early plant virus detection

2018 ◽  
Vol 173 (3) ◽  
pp. 285-293 ◽  
Author(s):  
John M. K. Roberts ◽  
Kylie B. Ireland ◽  
Wee T. Tay ◽  
Dean Paini
Keyword(s):  
Honey Bee ◽  
Plant Virus ◽  
Virus Detection ◽  
Plant Virus Detection

scholarly journals Application of Oxford Nanopore Technology to Plant Virus Detection

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1424
Author(s):  
Lia W. Liefting ◽  
David W. Waite ◽  
Jeremy R. Thompson
Keyword(s):  
Plant Virus ◽  
High Throughput Sequencing ◽  
Virus Detection ◽  
Diagnostic Methods ◽  
Plant Virus Detection ◽  
Sequencing Technologies ◽  
Oxford Nanopore ◽  
Virus Diagnostics ◽  
Post Entry ◽  
Read Accuracy

The adoption of Oxford Nanopore Technologies (ONT) sequencing as a tool in plant virology has been relatively slow despite its promise in more recent years to yield large quantities of long nucleotide sequences in real time without the need for prior amplification. The portability of the MinION and Flongle platforms combined with lowering costs and continued improvements in read accuracy make ONT an attractive method for both low- and high-scale virus diagnostics. Here, we provide a detailed step-by-step protocol using the ONT Flongle platform that we have developed for the routine application on a range of symptomatic post-entry quarantine and domestic surveillance plant samples. The aim of this methods paper is to highlight ONT’s feasibility as a valuable component to the diagnostician’s toolkit and to hopefully stimulate other laboratories towards the eventual goal of integrating high-throughput sequencing technologies as validated plant virus diagnostic methods in their own right.

scholarly journals Application of image recognition for plant virus detection

2021 ◽  
Vol 27 (S1) ◽  
pp. 2274-2276
Author(s):  
Min-Sheng Hung ◽  
Yi-Tzu Chiu
Keyword(s):  
Image Recognition ◽  
Plant Virus ◽  
Virus Detection ◽  
Plant Virus Detection

Plant Virus Detection in Animal Vectors

2001 ◽  
pp. 501-522
Author(s):  
Rudra P. Singh
Keyword(s):  
Plant Virus ◽  
Virus Detection ◽  
Plant Virus Detection

Developments in methodology of plant virus detection

1992 ◽  
Vol 98 (S2) ◽  
pp. 21-28 ◽  
Author(s):  
L. Torrance
Keyword(s):  
Plant Virus ◽  
Virus Detection ◽  
Plant Virus Detection

scholarly journals High Throughput Sequencing For Plant Virus Detection and Discovery

2019 ◽  
Vol 109 (5) ◽  
pp. 716-725 ◽  
Author(s):  
D. E. V. Villamor ◽  
T. Ho ◽  
M. Al Rwahnih ◽  
R. R. Martin ◽  
I. E. Tzanetakis
Keyword(s):  
High Throughput ◽  
Plant Virus ◽  
High Throughput Sequencing ◽  
Virus Detection ◽  
Agricultural Crops ◽  
Virus Discovery ◽  
Plant Virus Detection ◽  
Virus Biology ◽  
Disease Causality

Over the last decade, virologists have discovered an unprecedented number of viruses using high throughput sequencing (HTS), which led to the advancement of our knowledge on the diversity of viruses in nature, particularly unraveling the virome of many agricultural crops. However, these new virus discoveries have often widened the gaps in our understanding of virus biology; the forefront of which is the actual role of a new virus in disease, if any. Yet, when used critically in etiological studies, HTS is a powerful tool to establish disease causality between the virus and its host. Conversely, with globalization, movement of plant material is increasingly more common and often a point of dispute between countries. HTS could potentially resolve these issues given its capacity to detect and discover. Although many pipelines are available for plant virus discovery, all share a common backbone. A description of the process of plant virus detection and discovery from HTS data are presented, providing a summary of the different pipelines available for scientists’ utility in their research.

Recombinase polymerase amplification applied to plant virus detection and potential implications

2018 ◽  
Vol 546 ◽  
pp. 72-77 ◽  
Author(s):  
Binoy Babu ◽  
Francisco M. Ochoa-Corona ◽  
Mathews L. Paret
Keyword(s):  
Plant Virus ◽  
Virus Detection ◽  
Recombinase Polymerase Amplification ◽  
Plant Virus Detection

Simple method for plant virus detection: effect of antibody immobilization technique

2002 ◽  
Vol 105 (1) ◽  
pp. 141-146 ◽  
Author(s):  
P.M Boltovets ◽  
V.R Boyko ◽  
I.Yu Kostikov ◽  
N.S Dyachenko ◽  
B.A Snopok ◽  
...  
Keyword(s):  
Plant Virus ◽  
Virus Detection ◽  
Antibody Immobilization ◽  
Simple Method ◽  
Plant Virus Detection ◽  
Detection Effect

RECENT ADVANCES IN PLANT VIRUS DETECTION

2002 ◽  
pp. 113-116 ◽  
Author(s):  
S.M. Wong
Keyword(s):  
Plant Virus ◽  
Virus Detection ◽  
Plant Virus Detection ◽  
Recent Advances

scholarly journals Evaluation of three internal control primers for routine RTPCR plant virus detection

2006 ◽  
Vol 59 ◽  
pp. 374-374
Author(s):  
R. Thangavel ◽  
F.M. Ochoa-Corona ◽  
B.S.M. Lebas ◽  
J.Z. Tang ◽  
D.R. Elliott ◽  
...  
Keyword(s):  
Internal Control ◽  
Plant Virus ◽  
Virus Detection ◽  
Plant Virus Detection

Immunocapture-PCR for Plant Virus Detection

2008 ◽  
pp. 183-192 ◽  
Author(s):  
Vincent Mulholland
Keyword(s):  
Plant Virus ◽  
Virus Detection ◽  
Plant Virus Detection
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