A novel role for involvement of N‐methyl‐D‐aspartate (NMDA) glutamate receptors in sperm acrosome reaction

Andrologia ◽  
2021 ◽  
Author(s):  
Hamid Reza Momeni ◽  
Tahereh Etemadi ◽  
Atieh Alyasin ◽  
Najmeh Eskandari
Keyword(s):  
Glutamate Receptors ◽  
Acrosome Reaction ◽  
Nmda Glutamate Receptors ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

Native zona pellucida structure is required for completion of sperm acrosome reaction in porcine fertilization

1994 ◽  
Vol 41 (6) ◽  
pp. 1307-1313 ◽  
Author(s):  
M. Yoshizawa ◽  
T. Nagai ◽  
N. Yonezawa ◽  
M. Nakano
Keyword(s):  
Zona Pellucida ◽  
Acrosome Reaction ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

scholarly journals Polyphosphoinositide breakdown and subsequent exocytosis in the Ca2+/ionophore-induced acrosome reaction of mammalian spermatozoa

1989 ◽  
Vol 259 (2) ◽  
pp. 397-406 ◽  
Author(s):  
E R S Roldan ◽  
R A P Harrison
Keyword(s):  
Acrosome Reaction ◽  
Fertilization In Vitro ◽  
Ionophore A23187 ◽  
Bivalent Cation ◽  
Phosphoinositide Breakdown ◽  
Phosphoinositide Cycle ◽  
Phosphatidylinositol 4 ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

An investigation was made of the modifications in phospholipids that occur during the exocytotic event known as the ‘sperm acrosome reaction’. Phospholipids were prelabelled with 32P, and exocytosis was induced with Ca2+ and the ionophore A23187. When incubated with [32P]Pi in various media suitable for supporting sperm survival or fertilization in vitro, spermatozoa from all five species examined (ram, boar, guinea pig, mouse and human) incorporated 32P rapidly into the components of the phosphoinositide cycle. There were differences both between species and between media with respect to the actual rate of incorporation of label, and also between species with respect to other phospholipids labelled. Treatment of spermatozoa with Ca2+ and A23187 to induce the acrosome reaction resulted in a rapid breakdown of phosphatidylinositol 4, 5-bisphosphate and phosphatidylinositol 4-phosphate, which was complete within 3 min; there was also a great increase in labelling of phosphatidate. Occurrence of acrosome reactions in the sperm population was only observed after 5-10 min and reached a maximum response of greater than 90% after more than 30 min. The phosphoinositide breakdown was related to subsequent exocytosis: after EGTA/ionophore treatment, neither inositide breakdown nor exocytosis took place; however, later addition of Ca2+ resulted in immediate inositide breakdown, and exocytosis followed, with a delay relative to Ca2+ addition exactly similar to that following standard Ca2+/ionophore treatment. Neomycin inhibited both inositide breakdown and subsequent exocytosis provided it was added together with Ca2+ and ionophore; however, if the drug was added 3 min after Ca2+ and ionophore (by which time inositide breakdown was already complete), exocytosis was not inhibited. Ca2+ seemed to have several consecutive roles in the acrosome reaction. Low (micromolar) levels of free Ca2+ were needed both for phosphoinositide breakdown and for an event downstream of this breakdown; no other bivalent cation could substitute for Ca2+ in either event, and inositide breakdown was actually inhibited by Mg2+. In addition, millimolar levels of Ca2+ were needed for later stages of exocytosis, although this requirement could be satisfied by Sr2+. We conclude that breakdown of polyphosphoinositides is an essential early process after Ca2+ entry in the chain of events that lead to exocytosis in the mammalian sperm acrosome reaction.

scholarly journals Mammalian Sperm Acrosome Reaction: Where Does It Begin Before Fertilization?

2011 ◽  
Vol 85 (1) ◽  
pp. 4-5 ◽  
Author(s):  
Ryuzo Yanagimachi
Keyword(s):  
Acrosome Reaction ◽  
Mammalian Sperm ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

scholarly journals Sodium requirement for capacitation and membrane fusion during the guinea-pig sperm acrosome reaction

Reproduction ◽  
1984 ◽  
Vol 70 (1) ◽  
pp. 83-94 ◽  
Author(s):  
R. V. Hyne ◽  
R. E. Higginson ◽  
D. Kohlman ◽  
A. Lopata
Keyword(s):  
Guinea Pig ◽  
Membrane Fusion ◽  
Acrosome Reaction ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

scholarly journals Effect of acetylcholine on the human sperm acrosome reaction.

1996 ◽  
Vol 71 ◽  
pp. 257
Author(s):  
Akira Nozawa. ◽  
Koji Ogasawara. ◽  
Satoru Kaneko. ◽  
Koichi Takeuchi ◽  
Takao Hashimoto
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome
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