NO, CO and H2S: What about gasotransmitters in fish and amphibian heart?

2018 ◽  
Vol 223 (1) ◽  
pp. e13035 ◽  
Author(s):  
S. Imbrogno ◽  
M. Filice ◽  
M. C. Cerra ◽  
A. Gattuso
Keyword(s):  
1979 ◽  
Vol 11 (2) ◽  
pp. 231-248 ◽  
Author(s):  
Asish C. Nag ◽  
Christopher J. Healy ◽  
Mei Cheng

1994 ◽  
Vol 141 (2) ◽  
pp. 87-95 ◽  
Author(s):  
Martin Vila Petroff ◽  
Cecilia Mundiña-Weilenmann ◽  
Leticia Vittone ◽  
Gladys Chiappe de Cingolani ◽  
Alicia Mattiazzi

Author(s):  
Asish C. Nag ◽  
Christopher J. Healy ◽  
Mei Cheng

A long-term culture method of beating heart explants of adult newt has been developed recently in our laboratory. This method has enabled us to study the ultrastructure and contractile activities of beating adult heart explants at selected intervals. Adult newt heart ventricle was excised and washed with amphibian balanced salt solution and cut into several pieces of approximately 0. 5-1. 0 mm. Heart pieces were cultured for 2 months at 25°C in a medium containing 90% modified Leibovitz medium (L-15), 10% fetal calf serum and 1% penicillin-Streptomycin. In first week of culture, more than 37% of heart explants were attached to the substrate, and more than 33% established pulsation rates. Some explants had a rate of 3-12 beat/min., while others had a rate of 28-67 beat/min. A few cells migrated from the explants and were attached to the substrate around the explants. These migrated cells did not show pulsation.


2007 ◽  
Vol 210 (13) ◽  
pp. 2267-2277 ◽  
Author(s):  
C. Gaitanaki ◽  
T. Kalpachidou ◽  
I.-K. S. Aggeli ◽  
P. Papazafiri ◽  
I. Beis

2002 ◽  
Vol 205 (4) ◽  
pp. 443-454 ◽  
Author(s):  
Ioanna-Katerina S. Aggeli ◽  
Catherine Gaitanaki ◽  
Antigone Lazou ◽  
Isidoros Beis

SUMMARY We assessed the activation of p38-MAPK (mitogen-activated protein kinase) by osmotic and thermal stresses in the isolated perfused amphibian (Rana ridibunda) heart. Hyperosmotic stress induced the rapid activation of the kinase. In particular, in the presence of 0.5 mol l–1 sorbitol, p38-MAPK was maximally phosphorylated (by approximately twelvefold) at 15 min, while excess of NaCl (206 mmol l–1 final concentration) or KCl (16 mmol l–1 final concentration) stimulated a less potent activation, maximised (by approximately eightfold and fourfold) within 2 min and 30 s, respectively, relative to control values. The effect of all three compounds examined was reversible, since the kinase phosphorylation levels decreased upon reperfusion of the heart with normal bicarbonate-buffered saline. Conversely, hypotonicity did not induce any p38-MAPK activation. Furthermore, both hypothermia and hyperthermia induced considerable phosphorylation of the kinase, by four- and 7.5-fold, respectively, relative to control values. Immunohistochemical studies elucidated the localisation pattern of phospho-p38-MAPK and also revealed enhanced atrial natriuretic peptide (ANP) immunoreactivity in osmotically stressed hearts. Interestingly, SB 203580 (1 μmol l–1) not only completely blocked the activation of p38-MAPK by all these interventions, but also abolished the enhanced ANP immunoreactivity induced by 0.5 mol l–1 sorbitol. These findings indicate the possible involvement of ANP in the mechanisms regulating responses under such stressful conditions.


1942 ◽  
Vol 21 (1) ◽  
pp. 111???113
Author(s):  
John Adriani ◽  
E. A. Rovenstine
Keyword(s):  

2005 ◽  
Vol 16 (4-6) ◽  
pp. 183-192 ◽  
Author(s):  
Athanassios Vassilopoulos ◽  
Catherine Gaitanaki ◽  
Panagiota Papazafiri ◽  
Isidoros Beis

2006 ◽  
Vol 291 (1-2) ◽  
pp. 107-117 ◽  
Author(s):  
Catherine Gaitanaki ◽  
Maria Papatriantafyllou ◽  
Konstantina Stathopoulou ◽  
Isidoros Beis

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