ROLE OF FRESH HUMAN OR GUINEA-PIG SERUM IN THE INDIRECT FLUORESCENT ANTIBODY TEST FOR MYCOPLASMA PNEUMONIAE ANTIBODIES IN HUMAN SERA

1965 ◽  
Vol 63 (4) ◽  
pp. 639-640 ◽  
Author(s):  
K. Lind
Keyword(s):  
Guinea Pig ◽  
Mycoplasma Pneumoniae ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Indirect Fluorescent Antibody ◽  
Human Sera ◽  
Pig Serum

scholarly journals Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays

1978 ◽  
Vol 7 (5) ◽  
pp. 486-489
Author(s):  
S Stagno ◽  
D W Reynolds ◽  
R J Smith
Keyword(s):  
Human Cytomegalovirus ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Nuclear Antigen ◽  
Indirect Fluorescent Antibody ◽  
Isolated Nuclei ◽  
Antibody Assays ◽  
Human Sera ◽  
Antibody Levels

Use of an antigen consisting of purified isolated nuclei from a mixture of human cytomegalovirus-infected and uninfected fibroblasts in a 2:1 ratio is a simple and reliable method for eliminating nonspecific fluorescence associated with the presence of Fc-immunoglobulin G receptors in the cytoplasm of infected cells. The specificity obtained with this antigen on 100 normal human sera was 99, 100, and 98% when compared with microneutralization, anticomplement immunofluorescence, and conventional indirect fluorescent-antibody assays, respectively. Also, 95% of the antibody titers obtained with the nuclear antigen had a perfect correlation with or were within a fourfold-dilution difference of the antibody levels obtained by anticomplement immunofluorescence and the conventional indirect fluorescent-antibody test.

Investigation of Anti-Toxoplasma gondii Antibodies in Cats of the Ankara Region of Turkey Using the Sabin-Feldman Dye Test and an Indirect Fluorescent Antibody Test

2008 ◽  
Vol 94 (4) ◽  
pp. 817-820 ◽  
Author(s):  
Ayşegül Taylan Özkan ◽  
Bekir Çelebi ◽  
Cahit Babür ◽  
Araceli Lucio-Forster ◽  
Dwight D. Bowman ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Indirect Fluorescent Antibody
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