Identification of small peptides and glycinamide that inhibit melanin synthesis using a positional scanning synthetic peptide combinatorial library

2019 ◽  
Vol 181 (1) ◽  
pp. 128-137 ◽  
Author(s):  
J. H. Kim ◽  
J. K. Seok ◽  
Y. M. Kim ◽  
Y. C. Boo
Keyword(s):  
Synthetic Peptide ◽  
Combinatorial Library ◽  
Melanin Synthesis ◽  
Small Peptides ◽  
Positional Scanning

Identification of Antimelanogenic Peptides through Positional Scanning of a Synthetic Peptide Combinatorial Library

2016 ◽  
Vol 100 ◽  
pp. S81
Author(s):  
Jin Kyung Seok ◽  
Seok Won Lee ◽  
Young Mi Kim ◽  
Yong Chool Boo
Keyword(s):  
Synthetic Peptide ◽  
Combinatorial Library ◽  
Positional Scanning

scholarly journals Identification of novel antimelanogenic hexapeptides via positional scanning of a synthetic peptide combinatorial library

2017 ◽  
Vol 26 (8) ◽  
pp. 742-744 ◽  
Author(s):  
Jin K. Seok ◽  
Seok W. Lee ◽  
Jaehyuk Choi ◽  
Young M. Kim ◽  
Yong C. Boo
Keyword(s):  
Synthetic Peptide ◽  
Combinatorial Library ◽  
Positional Scanning

Positional scanning synthetic peptide combinatorial libraries

Peptides 1992 ◽  
1993 ◽  
pp. 65-66
Author(s):  
Clemencia Pinilla ◽  
Jon R. Appel ◽  
Richard A. Houghten
Keyword(s):  
Synthetic Peptide ◽  
Combinatorial Libraries ◽  
Positional Scanning

scholarly journals Non-combinatorial library screening reveals subsite cooperativity and identifies new high-efficiency substrates for kallikrein-related peptidase 14

2012 ◽  
Vol 393 (5) ◽  
pp. 331-341 ◽  
Author(s):  
Simon J. de Veer ◽  
Joakim E. Swedberg ◽  
Edward A. Parker ◽  
Jonathan M. Harris
Keyword(s):  
Binding Site ◽  
Combinatorial Library ◽  
High Efficiency ◽  
Sparse Matrix ◽  
Screening Methods ◽  
Optimal Sequence ◽  
Protease Cleavage ◽  
Positional Scanning ◽  
Active Site Cleft ◽  
Protease Substrate

Abstract An array of substrates link the tryptic serine protease, kallikrein-related peptidase 14 (KLK14), to physiological functions including desquamation and activation of signaling molecules associated with inflammation and cancer. Recognition of protease cleavage sequences is driven by complementarity between exposed substrate motifs and the physicochemical signature of an enzyme’s active site cleft. However, conventional substrate screening methods have generated conflicting subsite profiles for KLK14. This study utilizes a recently developed screening technique, the sparse matrix library, to identify five novel high-efficiency sequences for KLK14. The optimal sequence, YASR, was cleaved with higher efficiency (kcat/Km=3.81±0.4×106 m-1 s-1) than favored substrates from positional scanning and phage display by 2- and 10-fold, respectively. Binding site cooperativity was prominent among preferred sequences, which enabled optimal interaction at all subsites as indicated by predictive modeling of KLK14/substrate complexes. These simulations constitute the first molecular dynamics analysis of KLK14 and offer a structural rationale for the divergent subsite preferences evident between KLK14 and closely related KLKs, KLK4 and KLK5. Collectively, these findings highlight the importance of binding site cooperativity in protease substrate recognition, which has implications for discovery of optimal substrates and engineering highly effective protease inhibitors.

A Positional Scanning Combinatorial Library of Peptoids As a Source of Biological Active Molecules:  Identification of Antimicrobials

2003 ◽  
Vol 5 (5) ◽  
pp. 597-605 ◽  
Author(s):  
Marc Humet ◽  
Teresa Carbonell ◽  
Isabel Masip ◽  
Francisco Sánchez-Baeza ◽  
Puig Mora ◽  
...  
Keyword(s):  
Combinatorial Library ◽  
Positional Scanning

scholarly journals Identification of novel cyclic lipopeptides from a positional scanning combinatorial library with enhanced antibacterial and antibiofilm activities

2016 ◽  
Vol 108 ◽  
pp. 354-363 ◽  
Author(s):  
Nina Bionda ◽  
Renee M. Fleeman ◽  
César de la Fuente-Núñez ◽  
Maria C. Rodriguez ◽  
Fany Reffuveille ◽  
...  
Keyword(s):  
Combinatorial Library ◽  
Cyclic Lipopeptides ◽  
Positional Scanning

Kinetic characterization of a peptide inhibitor of trypsin isolated from a synthetic peptide combinatorial library

1995 ◽  
Vol 5 (6) ◽  
pp. 611-614 ◽  
Author(s):  
Gary S. Coombs ◽  
James Hazzard ◽  
David R. Corey
Keyword(s):  
Synthetic Peptide ◽  
Combinatorial Library ◽  
Peptide Inhibitor ◽  
Kinetic Characterization

Identification of related peptides recognized by a monoclonal antibody using a synthetic peptide combinatorial library

ImmunoMethods ◽  
1992 ◽  
Vol 1 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Jon R. Appel ◽  
Clemencia Pinilla ◽  
Richard A. Houghten
Keyword(s):  
Monoclonal Antibody ◽  
Synthetic Peptide ◽  
Combinatorial Library

Determination of multiple binding specificities of a monoclonal antibody using a synthetic peptide combinatorial library

Peptides 1992 ◽  
1993 ◽  
pp. 903-904
Author(s):  
Clemencia Pinilla ◽  
Jon R. Appel ◽  
Sandry Chendra ◽  
Richard A. Houghten
Keyword(s):  
Monoclonal Antibody ◽  
Synthetic Peptide ◽  
Combinatorial Library ◽  
Multiple Binding
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