Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

This case report seeks to describe efficient clinical application of adipose-derived stem cells (AdSCs) originated from buccal fat pad (BFP) in combination with conventional guided bone regeneration as protected healing space for reconstruction of large alveolar defects after extraction of multiple impacted teeth. The first case was a 19-year-old woman with several impacted teeth in the maxillary and mandibular regions, which could not be forced to erupt and were recommended for surgical extraction by the orthodontist. After this procedure, a large bone defect was created, and this space was filled by AdSC loaded natural bovine bone mineral (NBBM), which was protected with lateral ramus cortical plates, microscrews, and collagen membrane. After 6 months of post-guided bone regeneration, the patient received 6 and 7 implant placements, respectively, in the maxilla and mandible. At 10 months postoperatively, radiographic evaluation revealed thorough survival of implants. The second case was a 22-year-old man with the same complaint and large bony defects created after his teeth were extracted. After 6 months of post-guided bone regeneration, he received 4 dental implants in his maxilla and 7 implants in the mandible. At 48 months postoperatively, radiographs showed complete survival of implants. This approach represented a considerable amount of 3-dimensional bone formation in both cases, which enabled us to use dental implant therapy for rehabilitation of the whole dentition. The application of AdSCs isolated from BFP in combination with NBBM can be considered an efficient treatment for bone regeneration in large alveolar bone defects.

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Mesenchymal Stem Cells and Bovine Bone Mineral in Sinus Lift Procedures—An Experimental Study in Sheep

Tissue Engineering Part C Methods ◽

10.1089/ten.tec.2009.0734 ◽

2010 ◽

Vol 16 (5) ◽

pp. 1033-1039 ◽

Cited By ~ 21

Author(s):

Sebastian Sauerbier ◽

Kerstin Stubbe ◽

Michele Maglione ◽

Jörg Haberstroh ◽

Jens Kuschnierz ◽

...

Keyword(s):

Stem Cells ◽

Experimental Study ◽

Mesenchymal Stem Cells ◽

Bone Mineral ◽

Bovine Bone ◽

Sinus Lift ◽

Bovine Bone Mineral

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Lateral Ramus Cortical Bone Plate in Alveolar Cleft Osteoplasty with Concomitant Use of Buccal Fat Pad Derived Cells and Autogenous Bone: Phase I Clinical Trial

BioMed Research International ◽

10.1155/2017/6560234 ◽

2017 ◽

Vol 2017 ◽

pp. 1-12 ◽

Cited By ~ 12

Author(s):

Arash Khojasteh ◽

Lida Kheiri ◽

Hossein Behnia ◽

Azita Tehranchi ◽

Pantea Nazeman ◽

...

Keyword(s):

Clinical Trial ◽

Bone Formation ◽

Bone Defects ◽

Collagen Membrane ◽

Bovine Bone ◽

New Bone Formation ◽

Fat Pad ◽

Buccal Fat Pad ◽

Alveolar Cleft ◽

Bovine Bone Mineral

Tissue regeneration has become a promising treatment for craniomaxillofacial bone defects such as alveolar clefts. This study sought to assess the efficacy of lateral ramus cortical plate with buccal fat pad derived mesenchymal stem cells (BFSCs) in treatment of human alveolar cleft defects. Ten patients with unilateral anterior maxillary cleft met the inclusion criteria and were assigned to three treatment groups. First group was treated with anterior iliac crest (AIC) bone and a collagen membrane (AIC group), the second group was treated with lateral ramus cortical bone plate (LRCP) with BFSCs mounted on a natural bovine bone mineral (LRCP+BFSC), and the third group was treated with AIC bone, BFSCs cultured on natural bovine bone mineral, and a collagen membrane (AIC+BFSC). The amount of regenerated bone was measured using cone beam computed tomography 6 months postoperatively. AIC group showed the least amount of new bone formation (70±10.40%). LRCP+BFSC group demonstrated defect closure and higher amounts of new bone formation (75±3.5%) but less than AIC+BFSC (82.5±6.45%), suggesting that use of BFSCs within LRCP cage and AIC may enhance bone regeneration in alveolar cleft bone defects; however, the differences were not statistically significant. This clinical trial was registered at clinicaltrial.gov with NCT02859025 identifier.

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