Notes from rusty patched bumble bee ( Bombus affinis Cresson) nest observations

Estimation of the fructose diphosphatase–phosphofructokinase substrate cycle in the flight muscle of Bombus affinis

Biochemical Journal ◽

10.1042/bj1340589 ◽

1973 ◽

Vol 134 (2) ◽

pp. 589-597 ◽

Cited By ~ 108

Author(s):

Michael G. Clark ◽

David P. Bloxham ◽

Paul C. Holland ◽

Henry A. Lardy

Keyword(s):

Glucose Metabolism ◽

Ambient Temperature ◽

Calcium Ions ◽

Low Temperatures ◽

Flight Muscle ◽

Bumble Bee ◽

Bombus Affinis

1. Substrate cycling of fructose 6-phosphate through reactions catalysed by phosphofructokinase and fructose diphosphatase was estimated in bumble-bee (Bombus affinis) flight muscle in vivo. 2. Estimations of substrate cycling of fructose 6-phosphate and of glycolysis were made from the equilibrium value of the 3H/14C ratio in glucose 6-phosphate as well as the rate of 3H release to water after the metabolism of [5-3H,U-14C]glucose. 3. In flight, the metabolism of glucose proceeded exclusively through glycolysis (20.4μmol/min per g fresh wt.) and there was no evidence for substrate cycling. 4. In the resting bumble-bee exposed to low temperatures (5°C), the pattern of glucose metabolism in the flight muscle was altered so that substrate cycling was high (10.4μmol/min per g fresh wt.) and glycolysis was decreased (5.8μmol/min per g fresh wt.). 5. The rate of substrate cycling in the resting bumble-bee flight muscle was inversely related to the ambient temperature, since at 27°, 21° and 5°C the rates of substrate cycling were 0, 0.48 and 10.4μmol/min per g fresh wt. respectively. 6. Calcium ions inhibited fructose diphosphatase of the bumble-bee flight muscle at concentrations that were without effect on phosphofructokinase. The inhibition was reversed by the presence of a Ca2+-chelating compound. It is proposed that the rate of fructose 6-phosphate substrate cycling could be regulated by changes in the sarcoplasmic Ca2+ concentration associated with the contractile process.

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A century of pollen foraging by the endangered rusty patched bumble bee (Bombus affinis): inferences from molecular sequencing of museum specimens

Biodiversity and Conservation ◽

10.1007/s10531-020-02081-8 ◽

2020 ◽

Vol 30 (1) ◽

pp. 123-137

Author(s):

Michael P. Simanonok ◽

Clint R. V. Otto ◽

R. Scott Cornman ◽

Deborah D. Iwanowicz ◽

James P. Strange ◽

...

Keyword(s):

Bumble Bee ◽

Museum Specimens ◽

Pollen Foraging ◽

Bombus Affinis ◽

Molecular Sequencing

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Enzyme gene variation in five species of bumble bees (Hymenoptera: Apidae)

Canadian Journal of Zoology ◽

10.1139/z92-175 ◽

1992 ◽

Vol 70 (6) ◽

pp. 1263-1266 ◽

Cited By ~ 2

Author(s):

Victoria Bregazzi ◽

Terence Laverty

Keyword(s):

Genetic Variability ◽

Bumble Bees ◽

Bumble Bee ◽

Southern Ontario ◽

Enzyme Gene ◽

Gene Variation ◽

Expected Heterozygosity ◽

Enzyme Loci ◽

Bombus Affinis

Genetic variability at eight enzyme loci was assessed in five bumble bee species from southern Ontario: Bombus affinis Cresson, B. fervidus (Fabricius), B. bimaculatus Cresson, B. impatiens Cresson, and B. vagans Smith. Average expected heterozygosity (Hexp ± SE) across all species was 0.089 ± 0.023 (range 0.046–0.139). These values are higher than any previously reported estimates of average expected heterozygosities for bumble bees.

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