Expressional analysis of the silkworm storage protein 1 and identification of its interacting proteins

2019 ◽  
Vol 29 (1) ◽  
pp. 66-76 ◽  
Author(s):  
Ruilin Li ◽  
Congwu Hu ◽  
Tao Geng ◽  
Dingding Lv ◽  
Kun Gao ◽  
...  
Keyword(s):  
Storage Protein ◽  
Interacting Proteins ◽  
Expressional Analysis ◽  
Storage Protein 1

Anti-oxidative effects of silkworm storage protein 1 in HeLa cell

2017 ◽  
Vol 55 ◽  
pp. 199-206 ◽  
Author(s):  
Ji Hye Lee ◽  
Ji Eun Baik ◽  
Won Jong Rhee
Keyword(s):  
Hela Cell ◽  
Storage Protein ◽  
Storage Protein 1 ◽  
Oxidative Effects

scholarly journals Artificial selection on storage protein 1 possibly contributes to increase of hatchability during silkworm domestication

PLoS Genetics ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. e1007616 ◽  
Author(s):  
Ya-Nan Zhu ◽  
Li-Zhi Wang ◽  
Cen-Cen Li ◽  
Yong Cui ◽  
Man Wang ◽  
...  
Keyword(s):  
Artificial Selection ◽  
Storage Protein ◽  
Storage Protein 1

Distribution and accumulation of storage protein-1 in ovary ofHyphantria cunea Drury

1998 ◽  
Vol 37 (2) ◽  
pp. 115-128 ◽  
Author(s):  
Sook-Jae Seo ◽  
Young-Jin Kang ◽  
Hyang-Mi Cheon ◽  
Hak-Ryul Kim
Keyword(s):  
Storage Protein ◽  
Storage Protein 1

Expression and localization of storage protein 1 (SP1) in differentiated fat body tissues of red hairy caterpillar,Amsacta albistrigaWalker

2008 ◽  
Vol 69 (2) ◽  
pp. 70-84 ◽  
Author(s):  
Raman Chandrasekar ◽  
Seo Sook Jae ◽  
M. Krishnan
Keyword(s):  
Storage Protein ◽  
Fat Body ◽  
Body Tissues ◽  
Storage Protein 1

Sequestration of storage protein 1 (SP1) in differentiated fat body tissues of the female groundnut pest Amsacta albistriga (Lepidoptera: Arctiidae)

2008 ◽  
Vol 28 (02) ◽  
pp. 78 ◽  
Author(s):  
Raman Chandrasekar ◽  
Prusothaman Sumithra ◽  
Seo Sook Jae ◽  
Muthukalingan Krishnan
Keyword(s):  
Storage Protein ◽  
Fat Body ◽  
Body Tissues ◽  
Storage Protein 1 ◽  
Amsacta Albistriga

scholarly journals Silkworm Storage Protein 1 Inhibits Autophagy-Mediated Apoptosis

2019 ◽  
Vol 20 (2) ◽  
pp. 318 ◽  
Author(s):  
Su Kang ◽  
Won Rhee
Keyword(s):  
Cho Cells ◽  
Storage Protein ◽  
Mammalian Cells ◽  
High Stress ◽  
Reactive Oxygen Species Generation ◽  
Chinese Hamster ◽  
Nutrient Deficiency ◽  
New Strategy ◽  
Autophagy Pathway ◽  
Storage Protein 1

Autophagy is a natural physiological process, and it induces the lysosomal degradation of intracellular components in response to environmental stresses, including nutrient starvation. Although an adequate autophagy level helps in cell survival, excessive autophagy triggered by stress such as starvation leads to autophagy-mediated apoptosis. Chinese hamster ovary (CHO) cells are widely used for producing biopharmaceuticals, including monoclonal antibodies. However, apoptosis induced by high stress levels, including nutrient deficiency, is a major problem in cell cultures grown in bioreactors, which should be overcome. Therefore, it is necessary to develop a method for suppressing excessive autophagy and for maintaining an appropriate autophagy level in cells. Therefore, we investigated the effect of silkworm storage protein 1 (SP1), an antiapoptotic protein, on autophagy-mediated apoptosis. SP1-expressing CHO cells were generated to assess the effect and molecular mechanism of SP1 in suppressing autophagy. These cells were cultured under starvation conditions by treatment with Earle’s balanced salt solution (EBSS) to induce autophagy. We observed that SP1 significantly inhibited autophagy-mediated apoptosis by suppressing caspase-3 activation and reactive oxygen species generation. In addition, SP1 suppressed EBSS-induced conversion of LC3-I to LC3-II and the expression of autophagy-related protein 7. Notably, basal Beclin-1 level was significantly low in the SP1-expressing cells, indicating that SP1 regulated upstream events in the autophagy pathway. Together, these findings suggest that SP1 offers a new strategy for overcoming severe autophagy-mediated apoptosis in mammalian cells, and it can be used widely in biopharmaceutical production.

Anti-apoptotic effects of the alpha-helix domain of silkworm storage protein 1

2017 ◽  
Vol 22 (6) ◽  
pp. 671-678 ◽  
Author(s):  
Ji Eun Baik ◽  
Won Jong Rhee
Keyword(s):  
Storage Protein ◽  
Alpha Helix ◽  
Storage Protein 1 ◽  
Apoptotic Effects

Inhibition of Endoplasmic Reticulum Stress-induced Apoptosis by Silkworm Storage Protein 1

2018 ◽  
Vol 23 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Yeon Ju Cha ◽  
Ji Eun Baik ◽  
Won Jong Rhee
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Storage Protein ◽  
Storage Protein 1 ◽  
Induced Apoptosis
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