INTRAEPIDERMAL FREE NERVE ENDINGS IN GUINEA PIGS

1982 ◽  
Vol 9 (2) ◽  
pp. 107-116 ◽  
Author(s):  
Motoyuki Mihara ◽  
Ken Hashimoto ◽  
Masanobu Kumakiri
1982 ◽  
Vol 9 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Motoyuki Mihara ◽  
Ken Hashimoto ◽  
Masanobu Kumakiri

We have examined the initial innervation of the head skin in Xenopus laevis embryos which is by two classes of trigeminal mechanoreceptor with beaded ‘free’ nerve-endings. By recording receptive areas electrophysiologically and staining peripheral sensory neurites with horseradish peroxidase, we have shown that ‘movement detector’ neurites from one trigeminal ganglion do not normally cross the dorsal midline of the head to innervate areas of skin on the opposite side. However, if one trigeminal ganglion is removed before peripheral innervation starts, movement detector neurites from the intact side will now cross the midline to innervate contralateral skin. These observations suggest a specific competitive interaction between movement detector neurites during their innervation of head skin. The second class of receptor, ‘rapid transient’ detectors, have a different pattern of innervation, crossing the midline in both normal and operated animals.


1995 ◽  
Vol 12 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Shin-Ichi Terashima ◽  
Peng-Jia Jiang ◽  
Vinci Mizuhira ◽  
Hiroshi Hasegawa ◽  
Mitsuru Notoyat

1996 ◽  
Vol 4 (2) ◽  
pp. 111-115 ◽  
Author(s):  
P. Lobenhoffer ◽  
R. Biedert ◽  
E. Stauffer ◽  
Chr Lattermann ◽  
T. G. Gerich ◽  
...  

2008 ◽  
Vol 29 (1) ◽  
pp. 87-90 ◽  
Author(s):  
Miguel R. B. Moraes ◽  
Maria Luzete C. Cavalcante ◽  
José Alberto D. Leite ◽  
Francisco Valdecir Ferreira ◽  
Antônio Juvêncio O. Castro ◽  
...  

1965 ◽  
Vol 25 (3) ◽  
pp. 179-193 ◽  
Author(s):  
Frank S. Labella ◽  
Madhu Sanwal

Bovine posterior pituitary glands were homogenized in 10 per cent sucrose and fractionated by differential centrifugation. The following centrifugation procedure resulted in the most satisfactory separation: 1000 g for 15 minutes—nuclei, connective tissue, basement membranes with associated endothelium, giant nerve endings, and whole pituicytes; 4200 g for 15 minutes—free nerve endings, including Herring bodies; 17,000 g for 15 minutes—mitochondria; 68,000 g for 15 minutes—neurosecretory granules. Electron microscopic examination was carried out on whole tissue and on the isolated fractions. Isolated nerve endings were examined also by negative staining techniques. Isolated nerve endings retain an apparently normal complement of mitochondria, neurosecretory granules, and microvesicles ("synaptic" vesicles). The free nerve endings closely resemble those observed in sections of intact posterior pituitary tissue. Free microvesicles were not observed in any of the fractions isolated and apparently sediment at centrifugal forces higher than those employed in this study.


1987 ◽  
Vol 97 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Takemoto Shin ◽  
Shigeru Wada ◽  
Tadatsugu Maeyama ◽  
Shun Watanabe

Substance P (SP) immunoreactive nerve endings in the laryngeal mucosa were studied by PAP immunohistochemistry with light and electron microscopy. SP immunoreactive sensory endings were observed in the epithelium as intra-epithelial free nerve endings and taste bud-like structures. A small number of autonomic SP immunoreactive nerve fibers were observed running parallel to arterioles which were over 30 μm in diameter and terminated in glandular cells. Contrary to findings by silver impregnation, intraepithelial free nerve endings were more frequently observed on the lower surface of the vocal cord. The taste bud-like structures were classified into two different types: (1) simple terminations and (2) reticular terminations, according to the mode of the SP immunoreactive nerve fiber. Immature or degenerated taste bud-like structures in the larynx were assumed to be mechanical receptors because these receptors lacked outer taste pores and taste hairs.


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