Photoisomerization spectrum of urocanic acid in human skin and in vitro: effects of simulated solar and artificial ultraviolet radiation

The in vitro effects of insulin on glucose transport and dissimilation were studied in human skin. Slices of skin obtained post-mortem were incubated in phosphate and bicarbonate media with a glucose concentration of 5.6 mM. In both buffers, insulin increased the glucose uptake in non-diabetic skin without affecting the intracellular glucose content. Similar results were obtained for diabetic skin incubated in a phosphate buffer.In bicarbonate buffer, the basal rate of glucose utilization of skin from diabetics was lower than normal; this may have been the result of a defect in the phosphofructokinase reaction. Diabetic skin also had an increased responsiveness to insulin in bicarbonate buffer.Non-diabetic skin incubated in phosphate buffer had a lower basal rate of glucose uptake and a greater responsiveness to insulin than the same tissue incubated in bicarbonate buffer.It is concluded that (a) skin from diabetic humans incubated in bicarbonate buffer had a decreased basal rate of glucose utilization and increased responsiveness to insulin; (b) insulin stimulated both glucose transport and dissimilation in diabetic and non-diabetic human skin.

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Evaluation of Cutaneous Damage and Repair Following Acute Solar Ultraviolet Radiation Exposure: Experiments with an In Vitro Reconstructed Human Skin Model and Excised Human Skin

Applied In Vitro Toxicology ◽

10.1089/aivt.2015.0010 ◽

2015 ◽

Vol 1 (2) ◽

pp. 109-117 ◽

Cited By ~ 3

Author(s):

Alexander J. Armento ◽

Jonathan Oldach ◽

Gina Stolper ◽

Maxwell Li ◽

Michael A. Bachelor ◽

...

Keyword(s):

Ultraviolet Radiation ◽

Radiation Exposure ◽

Human Skin ◽

Skin Model ◽

Solar Ultraviolet Radiation ◽

Ultraviolet Radiation Exposure ◽

Reconstructed Human Skin ◽

Solar Ultraviolet ◽

Cutaneous Damage

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An automated in vitro dermal absorption procedure: I. Permeation of 14C-labelled N,N-diethyl-m-toluamide through human skin and effects of short-wave ultraviolet radiation on permeation

Toxicology in Vitro ◽

10.1016/0887-2333(90)90021-k ◽

1990 ◽

Vol 4 (3) ◽

pp. 193-199 ◽

Cited By ~ 19

Author(s):

R.P. Moody ◽

P.A. Martineau

Keyword(s):

Ultraviolet Radiation ◽

Human Skin ◽

Short Wave ◽

Dermal Absorption

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Desmosomal distribution in the epidermis of a non-contracting human skin equivalent

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124884 ◽

1992 ◽

Vol 50 (1) ◽

pp. 896-897

Author(s):

L.X. Oakford ◽

S.D. Dimitrijevich ◽

R. Gracy

Keyword(s):

Human Skin ◽

Basal Layer ◽

Skin Equivalent ◽

Tissue Component ◽

Intact Skin ◽

Similar Sequence ◽

Sequence Of Events ◽

Suprabasal Keratinocytes ◽

Human Skin Equivalent

In intact skin the epidermal layer is a dynamic tissue component which is maintained by a basal layer of mitotically active cells. The protective upper epidermis, the stratum corneum, is generated by differentiation of the suprabasal keratinocytes which eventually desquamate as anuclear comeocytes. A similar sequence of events is observed in vitro in the non-contracting human skin equivalent (HSE) which was developed in this lab (1). As a part of the definition process for this model of living skin we are examining its ultrastructural features. Since desmosomes are important in maintaining cell-cell interactions in stratified epithelia their distribution in HSE was examined.

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