Stimulatory cholinergic effect on the release of antiaggregatory activity into the circulation of cat and man and its modification by β-adrenergic antagonists

We have been interested in the use of neural transplants mainly as a local source of neuroactive substances, rather than as a replacement for damaged neural circuities. In particular, we have been exploring the possibilities of reducing pain by transplants of opioid peptide producing cells, and reducing depression by transplants of monoamine-producing cells. For the past several years, work in our laboratory has demonstrated in both acute and chronic pain models that transplantation of adrenal medullary tissue or isolated chromaffin cells into CNS pain modulatory regions can reduce pain sensitivity in rodents. Chromaffin cells were chosen as donor source since they produce high levels of both opioid peptides and catecholamines, substances which independently, and probably synergistically, reduce pain sensitivity when injected locally into the spinal cord. The analgesia produced by these transplants most likely results from the release of both opioid peptides and catecholamines, since it can be blocked or attenuated by opiate or adrenergic antagonists, respectively. Furthermore, CSF levels of met-enkephalin and catecholamines are increased by the transplants.

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Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1653438 ◽

1981 ◽

Vol 46 (03) ◽

pp. 645-647 ◽

Cited By ~ 28

Author(s):

M A Orchard ◽

C Robinson

Keyword(s):

Platelet Aggregation ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Protective Effect ◽

Whole Blood ◽

Bovine Serum ◽

Fatty Acid Content ◽

Krebs Solution ◽

Antiaggregatory Activity ◽

Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

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Alpha-1 Adrenergic Antagonists Induced Severe Rhinitis in Patients with Benign Prostatic Hyperplasia

Current Drug Safety ◽

10.2174/1574886309666140527112408 ◽

2014 ◽

Vol 9 (2) ◽

pp. 159-160 ◽

Cited By ~ 2

Author(s):

Eduardo Shahar ◽

Laila Nassar ◽

Eynat Kedem ◽

Gammal Hassoun

Keyword(s):

Benign Prostatic Hyperplasia ◽

Prostatic Hyperplasia ◽

Adrenergic Antagonists

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Blockade of Vasospastic Attacks by α2-Adrenergic but Not α1-Adrenergic Antagonists in Idiopathic Raynaud’s Disease

Circulation ◽

10.1161/01.cir.92.6.1448 ◽

1995 ◽

Vol 92 (6) ◽

pp. 1448-1451 ◽

Cited By ~ 63

Author(s):

Robert R. Freedman ◽

Rachel P. Baer ◽

Maureen D. Mayes

Keyword(s):

Raynaud's Disease ◽

Raynaud’S Disease ◽

Adrenergic Antagonists

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ANTAGONISM BETWEEN INSULIN AND SELECTIVE ADRENERGIC AGONISTS ON THE GLYCOGEN SYNTHETASE AND PHOSPHORYLASE SYSTEMS OF THE ISOLATED RAT DIAPHRAGM

Acta Endocrinologica ◽

10.1530/acta.0.0780392 ◽

1975 ◽

Vol 78 (2) ◽

pp. 392-400

Author(s):

Arne T. Hostmark ◽

Ole Grønnerød ◽

Robert S. Horn

Keyword(s):

Glycogen Metabolism ◽

Rat Diaphragm ◽

Adrenergic Agonists ◽

Adrenergic Stimulation ◽

Insulin Effect ◽

Glycogen Synthetase ◽

Adrenergic Antagonists ◽

Fasted Rats ◽

Isolated Rat ◽

Stimulation Of

ABSTRACT The antagonism between insulin and selective adrenergic stimulation on the converting systems for glycogen synthetase and phosphorylase has been investigated in the isolated rat diaphragm. Insulin significantly inhibited stimulation by terbutaline and noradrenaline of phosphorylase b to a conversion as well as stimulation of glycogen synthetase I to D conversion by these agents. The inhibition by insulin was stronger on the synthetase system than on the phosphorylase system. The insulin effect was not dependent upon the presence of glucose. In diaphragms from 24 h fasted rats the response of the phosphorylase system to both agonists decreased. Inhibition by insulin of terbutaline stimulated phosphorylase conversion was maintained upon fasting while no effect of insulin against stimulation by noradrenaline could be obtained in diaphragms from fasted rats. The effects of fasting and insulin were not influenced by beta adrenergic antagonists (practolol and butoxamine). The results indicate a difference in sensitivity of the synthetase and phosphorylase systems to insulin and suggest that noradrenaline and terbutaline influence glycogen metabolism by differing mechanisms.

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