Isolation and characterization of nonpathogenic Fusarium oxysporum isolates from the rhizosphere of healthy banana plants

Abstract. Wiratno, Syakir M, Sucipto I, Pradana AP. 2019. Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita. Biodiversitas 20: 682-687. The endophytic bacteria from spices plants have potential as biocontrol agents. Nevertheless, their potential has not been explored. Pepper plant (Piper nigrum L.) is linked with promising endophytic bacteria as biocontrol agents. This study aimed to obtain isolates of endophytic bacteria from root tissues of pepper plant. The isolation of endophytic bacteria was done using surface-sterilization method and using Tryptone Soya Agar (TSA) medium. The result showed that 10 endophytic bacteria were successfully isolated. We found that a total of 9 bacteria were safe for plants and mammals and used for subsequent steps. The selected bacteria showed inhibition activity of Fusarium oxysporum (18.6 to 43.7%). Furthermore, the secondary metabolites of the endophytic bacteria also promoted lethal effects on Meloidogyne incognita (16.6 to 65.8%). The physiological activity also showed that seven isolates were able to produce chitinase, and four (4) isolates were able to produce protease. Additionally, 55.6% of isolates were also able to dissolve phosphorus and fix nitrogen. This study provided fundamental information related to the biocontrol properties of endophytic bacteria isolated from pepper plant roots.

Download Full-text

Isolation and characterization of lipoxygenase from Fusarium oxysporum

Bio-Research ◽

10.4314/br.v3i2.28583 ◽

2006 ◽

Vol 3 (2) ◽

Author(s):

MN Anokwulu ◽

CU Anyanwu ◽

OR Offohndu

Keyword(s):

Fusarium Oxysporum ◽

Isolation And Characterization

Download Full-text

An Assessment Of Physicochemical Properties, Heavy Metal Enrichment And Fungal Characterization Of Refined Kerosene Impacted Soil In Anand, Gujarat, India

International Journal of Environment ◽

10.3126/ije.v2i1.9219 ◽

2013 ◽

Vol 2 (1) ◽

pp. 164-174 ◽

Cited By ~ 1

Author(s):

Shamiyan R Khan ◽

JI Nirmal Kumar ◽

Rita N Kumar ◽

Jignasha G Patel

Keyword(s):

Heavy Metal ◽

Physicochemical Properties ◽

Fusarium Oxysporum ◽

Contaminated Soil ◽

Garden Soil ◽

Metal Enrichment ◽

Isolation And Characterization ◽

Uncontaminated Soil ◽

Heavy Metal Enrichment

The present study was carried out to assess the physico-chemical properties, heavy metal enrichment and fungal isolation and characterization of the top soil samples collected in-situ from aged refined kerosene contaminated as well as uncontaminated garden soil sites in Anand, Gujarat, India. The total petroleum hydrocarbon (TPH) concentrations were 17,510 mg/kg in kerosene contaminated soil against 142.65 mg/kg for uncontaminated soils. The contamination increased the soil organic carbon, nitrogen and clay to 2.95 %, 0.612 %, 36.22 % as compared to 1.5%, 0.153%, 32.4% respectively in the uncontaminated soil. Increased concentration of heavy metals like Cobalt, Copper, Iron, Zinc and Lead against the uncontaminated soil was encountered. Ten native fungal speciesbelonging to a total of five genera include Aspergillus (A. terreus, A. versicolor, A. niger); Fusarium oxysporum; Penicilliumjanthinellum from the uncontaminated garden soil, whereas the contaminated soil included Aspergillus (A. terreus, A. versicolor , A. niger) Candida tropicalis,Cladosporiumbruhnei and Fusarium oxysporum, identified based on 18S rRNA and the nucleotide sequences were submitted to the NCBI, GenBank database. The changes created by kerosene contamination resulted in variation in individual concentrations of physicochemical properties, soil conductivity, pH and soil fertility indices probably dwindle the growth of fungal strains causing a reduction in the fungal population in the kerosene contaminated soil. International Journal of Environment, Volume-2, Issue-1, Sep-Nov 2013, Pages 164-174 DOI: http://dx.doi.org/10.3126/ije.v2i1.9219

Download Full-text

Isolation and characterization of a Fusarium oxysporum-resistant gene LrGLP1 from Lilium regale Wilson

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-017-9829-2 ◽

2017 ◽

Vol 53 (5) ◽

pp. 461-468 ◽

Cited By ~ 7

Author(s):

Nannan Zhang ◽

Ruipan Guan ◽

Ye Yang ◽

Zhiwei Bai ◽

Fe Ge ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Resistant Gene ◽

Isolation And Characterization ◽

Lilium Regale ◽

Lilium Regale Wilson

Download Full-text

Isolation and characterization of an exopolygalacturonase from Fusarium oxysporum f.sp. cubense race 1 and race 4

BMC Biochemistry ◽

10.1186/1471-2091-12-51 ◽

2011 ◽

Vol 12 (1) ◽

pp. 51 ◽

Cited By ~ 13

Author(s):

Zhangyong Dong ◽

Zhenzhong Wang

Keyword(s):

Fusarium Oxysporum ◽

Isolation And Characterization ◽

Race 1 ◽

Race 4

Download Full-text

Isolation and characterization of Fusarium oxysporum f. Sp. albedinis causing bayoud disease of date palm tree from Gambat, district Khairpur, Sindh, Pakistan

Plant Protection ◽

10.33804/pp.004.01.3234 ◽

2020 ◽

Vol 4 (1) ◽

Author(s):

Mirza Hussain Samo ◽

Noor Jehan Ismailli

Keyword(s):

Fusarium Oxysporum ◽

Date Palm ◽

Preventive Measures ◽

Morphological Characteristics ◽

Palm Tree ◽

Palm Trees ◽

Isolation And Characterization ◽

Significant Damage ◽

Bayoud Disease

Among different diseases of dates, bayoud disease caused by Fusarium oxysporum f. sp. albedinis causes serious damage to date palm throughout the world. The disease has damaged numerous date groves and can sometimes take on spectacular proportions when the disease appears in epidemic form. The disease is widespread and results in significant damage and incurs huge losses. In Pakistan, date palm is mainly cultivated in Khairpur (Sindh) and Turbat (Balochistan) districts. Date palm holds a significant position in the socio-economic development at Khairpur where several thousand peoples are associated directly or indirectly with date palm cultivation, trade and market. As no information exists about the bayoud disease in Khairpur district, therefore, in the present study, the disease was characterized on date palm trees caused by Fusarium oxysporum f. sp. albedinis at college area of Gambat, District Khairpur, Sindh, Pakistan. Samples of infected date palm trees of different varieties were collected from experimental area at the time of harvesting. The fungus was isolated, purified and identified as Fusarium oxysporum f. sp. albedinis on the basis of morphological characteristics. In conclusion, bayoud disease is an epiphytic disease for which there is no known cure at present. Only preventive measures could protect healthy date plantations from this disease.

Download Full-text