Isolation and characterization of a Fusarium oxysporum-resistant gene LrGLP1 from Lilium regale Wilson

2017 ◽  
Vol 53 (5) ◽  
pp. 461-468 ◽  
Author(s):  
Nannan Zhang ◽  
Ruipan Guan ◽  
Ye Yang ◽  
Zhiwei Bai ◽  
Fe Ge ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Resistant Gene ◽  
Isolation And Characterization ◽  
Lilium Regale ◽  
Lilium Regale Wilson

scholarly journals Lilium Regale Wilson WRKY3 Modulates an Antimicrobial Peptide Gene, Lrdef1, During Response to Fusarium Oxysporum

2021 ◽  
Author(s):  
Zie Wang ◽  
Jie Deng ◽  
Tingting Liang ◽  
Linlin Su ◽  
Lilei Zheng ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Fusarium Oxysporum ◽  
Transgenic Tobacco ◽  
Sequencing Analysis ◽  
Lilium Regale ◽  
Expression Activity ◽  
Metabolite Synthesis ◽  
Lilium Regale Wilson ◽  
Peptide Gene ◽  
Higher Sensitivity

Abstract Background: WRKY transcription factors (TFs) play vital roles in plant growth and development, secondary metabolite synthesis, and response to biotic and abiotic stresses. In a previous transcriptome sequencing analysis of Lilium regale Wilson, we identified multiple WRKY TFs that respond to exogenous methyl jasmonate treatment and lily Fusarium wilt (Fusarium oxysporum).Results: In the present study, the WRKY TF LrWRKY3 was further analyzed to reveal its function in defense response to F. oxysporum. The LrWRKY3 protein was localized in the plant cell nucleus, and LrWRKY3 transgenic tobacco lines showed higher resistance to F. oxysporum compared with wild-type (WT) tobacco. In addition, some genes related to jasmonic acid (JA) biosynthesis, salicylic acid (SA) signal transduction, and disease resistance had higher transcriptional levels in the LrWRKY3 transgenic tobacco lines than in the WT. On the contrary, L. regale scales transiently expressing LrWRKY3 RNA interference fragments showed higher sensitivity to F. oxysporum infection. Moreover, a F. oxysporum-induced defensin gene, Def1, was isolated from L. regale, and the recombinant protein LrDef1 isolated and purified from Escherichia coli possessed antifungal activity to several phytopathogens, including F. oxysporum. Furthermore, co-expression of LrWRKY3 and the LrDef1 promoter in tobacco evidently up-regulated the expression activity of the LrDef1 promoter.Conclusions: These results clearly indicate that LrWRKY3 is an important positive regulator in response to F. oxysporum infection, and one of its targets is the antimicrobial peptide gene LrDef1.

scholarly journals Isolation and Characterization of Antagonist Bacteria from Chickpea Rhizosphere against Fusarium oxysporum f. sp. ciceris

2017 ◽  
Vol 11 (1) ◽  
pp. 371-378
Author(s):  
KP Suthar ◽  
RM Patel ◽  
D Singh ◽  
VB Parekh ◽  
MD Khunt ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita

2019 ◽  
Vol 20 (3) ◽  
pp. 682-687 ◽  
Author(s):  
WIRATNO WIRATNO ◽  
MUHAMMAD SYAKIR ◽  
IRWANTO SUCIPTO ◽  
ANKARDIANSYAH PANDU PRADANA
Keyword(s):  
Fusarium Oxysporum ◽  
Meloidogyne Incognita ◽  
Endophytic Bacteria ◽  
Physiological Activity ◽  
Biocontrol Agents ◽  
Piper Nigrum ◽  
Pepper Plant ◽  
Surface Sterilization ◽  
Isolation And Characterization

Abstract. Wiratno, Syakir M, Sucipto I, Pradana AP. 2019. Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita. Biodiversitas 20: 682-687. The endophytic bacteria from spices plants have potential as biocontrol agents. Nevertheless, their potential has not been explored. Pepper plant (Piper nigrum L.) is linked with promising endophytic bacteria as biocontrol agents. This study aimed to obtain isolates of endophytic bacteria from root tissues of pepper plant. The isolation of endophytic bacteria was done using surface-sterilization method and using Tryptone Soya Agar (TSA) medium. The result showed that 10 endophytic bacteria were successfully isolated. We found that a total of 9 bacteria were safe for plants and mammals and used for subsequent steps. The selected bacteria showed inhibition activity of Fusarium oxysporum (18.6 to 43.7%). Furthermore, the secondary metabolites of the endophytic bacteria also promoted lethal effects on Meloidogyne incognita (16.6 to 65.8%). The physiological activity also showed that seven isolates were able to produce chitinase, and four (4) isolates were able to produce protease. Additionally, 55.6% of isolates were also able to dissolve phosphorus and fix nitrogen. This study provided fundamental information related to the biocontrol properties of endophytic bacteria isolated from pepper plant roots.

scholarly journals Isolation and characterization of lipoxygenase from Fusarium oxysporum

Bio-Research ◽  
2006 ◽  
Vol 3 (2) ◽  
Author(s):  
MN Anokwulu ◽  
CU Anyanwu ◽  
OR Offohndu
Keyword(s):  
Fusarium Oxysporum ◽  
Isolation And Characterization

scholarly journals An Assessment Of Physicochemical Properties, Heavy Metal Enrichment And Fungal Characterization Of Refined Kerosene Impacted Soil In Anand, Gujarat, India

2013 ◽  
Vol 2 (1) ◽  
pp. 164-174 ◽  
Author(s):  
Shamiyan R Khan ◽  
JI Nirmal Kumar ◽  
Rita N Kumar ◽  
Jignasha G Patel
Keyword(s):  
Heavy Metal ◽  
Physicochemical Properties ◽  
Fusarium Oxysporum ◽  
Contaminated Soil ◽  
Garden Soil ◽  
Metal Enrichment ◽  
Isolation And Characterization ◽  
Uncontaminated Soil ◽  
Heavy Metal Enrichment

The present study was carried out to assess the physico-chemical properties, heavy metal enrichment and fungal isolation and characterization of the top soil samples collected in-situ from aged refined kerosene contaminated as well as uncontaminated garden soil sites in Anand, Gujarat, India. The total petroleum hydrocarbon (TPH) concentrations were 17,510 mg/kg in kerosene contaminated soil against 142.65 mg/kg for uncontaminated soils. The contamination increased the soil organic carbon, nitrogen and clay to 2.95 %, 0.612 %, 36.22 % as compared to 1.5%, 0.153%, 32.4% respectively in the uncontaminated soil. Increased concentration of heavy metals like Cobalt, Copper, Iron, Zinc and Lead against the uncontaminated soil was encountered. Ten native fungal speciesbelonging to a total of five genera include Aspergillus (A. terreus, A. versicolor, A. niger); Fusarium oxysporum; Penicilliumjanthinellum from the uncontaminated garden soil, whereas the contaminated soil included Aspergillus (A. terreus, A. versicolor , A. niger) Candida tropicalis,Cladosporiumbruhnei and Fusarium oxysporum, identified based on 18S rRNA and the nucleotide sequences were submitted to the NCBI, GenBank database. The changes created by kerosene contamination resulted in variation in individual concentrations of physicochemical properties, soil conductivity, pH and soil fertility indices probably dwindle the growth of fungal strains causing a reduction in the fungal population in the kerosene contaminated soil. International Journal of Environment, Volume-2, Issue-1, Sep-Nov 2013, Pages 164-174 DOI: http://dx.doi.org/10.3126/ije.v2i1.9219

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