scholarly journals Molecular characterization of the CP gene and 3'UTR of Chilli veinal mottle virus from South and Southeast Asia

2008 ◽  
Vol 57 (3) ◽  
pp. 408-416 ◽  
Author(s):  
W. S. Tsai ◽  
Y. C. Huang ◽  
D. Y. Zhang ◽  
K. Reddy ◽  
S. H. Hidayat ◽  
...  
Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 651-663 ◽  
Author(s):  
Dragana Milosevic ◽  
Ivana Stankovic ◽  
Aleksandra Bulajic ◽  
Maja Ignjatov ◽  
Zorica Nikolic ◽  
...  

During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P1,2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.


2015 ◽  
Vol 60 (3) ◽  
Author(s):  
Jayanta Kumar Chamuah ◽  
Aseno Sakhrie ◽  
Sanjeevini Lama ◽  
Subhas Chandra ◽  
Gajanan M. Chigure ◽  
...  

AbstractMithun is a strongly built hill animal of Southeast Asia and plays an important role in the socio-economic and cultural life of the tribal population. Setaria digitata isolated from peritoneal cavity of mithun both from Arunachal Pradesh and Nagaland were characterized based on conserved region of 12SrDNA, 28SrDNA and ITS-2 and mitochondrial gene CoxI. Based on sequence analysis, it was found to be 99% similarity with Srilankan isolate of S. digitata.


2012 ◽  
Vol 03 (08) ◽  
pp. 971-978 ◽  
Author(s):  
Shahid Ali ◽  
Masood Akhtar ◽  
Kangabam S. Singh ◽  
Qamar A. Naqvi

2021 ◽  
Author(s):  
Yongliang Hu ◽  
Yuqin Chen ◽  
Xiaoxia Su ◽  
Jiawei Huang ◽  
Hongxing Yin ◽  
...  

Abstract The present study reports observation of a field chilli pepper disease consisting of a co-infection with two potyviruses: Wild tomato mosaic virus Dehong isolate (WTMV-Dh) and Chili veinal mottle virus Dehong isolate (ChiVMV-Dh). We obtained the complete genome sequences of these two viruses by NGS sequencing. The WTMV-Dh is 9,598 nucleotides (nt) in length and encodes a complete polyprotein of 3,075 amino acids (aa). The polyprotein of WTMV-Dh shares 76.1–82.6% nt and 85.3–89.5% aa identities with the other three WTMV isolates reported previously. The ChiVMV-Dh is 9688 nt in length and encodes a complete polyprotein with 3, 089 aa. The polyprotein of ChiVMV-Dh shares 80.8–92.2% nt and 85.3–95.6% aa identities with the other ChiVMV isolates reported previously. Following phylogenetic analysis based on the polyprotein sequences of other potyviruses, WTMV-Dh clustered with the Vietnam strain WTMV-Laichau while ChiVMV-Dh clustered with several ChiVMV Sichuan isolates. Evaluation of the recombination events within the WTMV and ChiVMV subgroups indicated that some putative recombination events occurred in critical regions. These regions include the N-terminal of HC-Pro and P1 region of WTMV-Dh, CP and the P3 to CI region of ChiVMV-Dh, which may be new evidence of adaptive evolution of potyviruses.


VirusDisease ◽  
2014 ◽  
Vol 25 (3) ◽  
pp. 331-337 ◽  
Author(s):  
K. Soumya ◽  
M. Yogita ◽  
Y. Prasanthi ◽  
K. Anitha ◽  
P. B. Kavi Kishor ◽  
...  

1998 ◽  
Vol 79 (9) ◽  
pp. 2275-2281 ◽  
Author(s):  
Y P Zhang ◽  
J K Uyemoto ◽  
C D Smart ◽  
B C Kirkpatrick

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