The upstream open reading frame of the Arabidopsis AtMHX gene has a strong impact on transcript accumulation through the nonsense-mediated mRNA decay pathway

2009 ◽  
Vol 60 (6) ◽  
pp. 1031-1042 ◽  
Author(s):  
Helen Saul ◽  
Einat Elharrar ◽  
Rachel Gaash ◽  
Dror Eliaz ◽  
Meital Valenci ◽  
...  
Keyword(s):  
Mrna Decay ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Strong Impact ◽  
Transcript Accumulation ◽  
Reading Frame ◽  
Nonsense Mediated Mrna Decay

scholarly journals Quality control of transcription start site selection by nonsense-mediated-mRNA decay

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Christophe Malabat ◽  
Frank Feuerbach ◽  
Laurence Ma ◽  
Cosmin Saveanu ◽  
Alain Jacquier
Keyword(s):  
Quality Control ◽  
Rna Polymerase Ii ◽  
Mrna Decay ◽  
Rna Degradation ◽  
Upstream Open Reading Frame ◽  
Start Codon ◽  
Messenger Rnas ◽  
Transcription Start ◽  
Reading Frame ◽  
Nonsense Mediated Mrna Decay

Nonsense-mediated mRNA decay (NMD) is a translation-dependent RNA quality-control pathway targeting transcripts such as messenger RNAs harboring premature stop-codons or short upstream open reading frame (uORFs). Our transcription start sites (TSSs) analysis of Saccharomyces cerevisiae cells deficient for RNA degradation pathways revealed that about half of the pervasive transcripts are degraded by NMD, which provides a fail-safe mechanism to remove spurious transcripts that escaped degradation in the nucleus. Moreover, we found that the low specificity of RNA polymerase II TSSs selection generates, for 47% of the expressed genes, NMD-sensitive transcript isoforms carrying uORFs or starting downstream of the ATG START codon. Despite the low abundance of this last category of isoforms, their presence seems to constrain genomic sequences, as suggested by the significant bias against in-frame ATGs specifically found at the beginning of the corresponding genes and reflected by a depletion of methionines in the N-terminus of the encoded proteins.

scholarly journals The canonical UPF1-dependent nonsense-mediated mRNA decay is inhibited in transcripts carrying a short open reading frame independent of sequence context

RNA ◽  
2006 ◽  
Vol 12 (12) ◽  
pp. 2160-2170 ◽  
Author(s):  
A. L. Silva ◽  
F. J.C. Pereira ◽  
A. Morgado ◽  
J. Kong ◽  
R. Martins ◽  
...  
Keyword(s):  
Mrna Decay ◽  
Open Reading Frame ◽  
Sequence Context ◽  
Reading Frame ◽  
Short Open Reading Frame ◽  
Nonsense Mediated Mrna Decay

scholarly journals Stress-sensitive Regulation of IFRD1 mRNA Decay Is Mediated by an Upstream Open Reading Frame

2010 ◽  
Vol 285 (12) ◽  
pp. 8552-8562 ◽  
Author(s):  
Chenyang Zhao ◽  
Shyamasree Datta ◽  
Palash Mandal ◽  
Shuqing Xu ◽  
Thomas Hamilton
Keyword(s):  
Mrna Decay ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Reading Frame

scholarly journals Long Open Reading Frame Transcripts Escape Nonsense-Mediated mRNA Decay in Yeast

Cell Reports ◽  
2014 ◽  
Vol 6 (4) ◽  
pp. 593-598 ◽  
Author(s):  
Laurence Decourty ◽  
Antonia Doyen ◽  
Christophe Malabat ◽  
Emmanuel Frachon ◽  
Delphine Rispal ◽  
...  
Keyword(s):  
Mrna Decay ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Nonsense Mediated Mrna Decay

scholarly journals Sucrose Control of Translation Mediated by an Upstream Open Reading Frame-Encoded Peptide

2009 ◽  
Vol 150 (3) ◽  
pp. 1356-1367 ◽  
Author(s):  
Fatemeh Rahmani ◽  
Maureen Hummel ◽  
Jolanda Schuurmans ◽  
Anika Wiese-Klinkenberg ◽  
Sjef Smeekens ◽  
...  
Keyword(s):  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Reading Frame

scholarly journals Translational regulation of Arabidopsis XIPOTL1 is modulated by phosphocholine levels via the phylogenetically conserved upstream open reading frame 30

2012 ◽  
Vol 63 (14) ◽  
pp. 5203-5221 ◽  
Author(s):  
Fulgencio Alatorre-Cobos ◽  
Alfredo Cruz-Ramírez ◽  
Celine A. Hayden ◽  
Claudia-Anahí Pérez-Torres ◽  
Anne-Laure Chauvin ◽  
...  
Keyword(s):  
Translational Regulation ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Reading Frame
Sign in / Sign up

Export Citation Format

Share Document