scholarly journals Phospholipase C from Bacillus cereus and Its Use in Studies of Tissue Thromboplastin

1972 ◽  
Vol 27 (2) ◽  
pp. 238-243 ◽  
Author(s):  
Anne-Brit Otnaess ◽  
Hans Prydz ◽  
Eirik Bjorklid ◽  
Ase Berre
Keyword(s):  
Bacillus Cereus ◽  
Phospholipase C ◽  
Tissue Thromboplastin

Studies on phosphatidylinositol phosphodiesterase (phospholipase C type) of Bacillus cereus

1976 ◽  
Vol 450 (2) ◽  
pp. 154-164 ◽  
Author(s):  
Ikezawa Hiroh ◽  
Yamanegi Masato ◽  
Taguchi Ryo ◽  
Miyashita Tomoyuki ◽  
Ohyabu Tetsuo
Keyword(s):  
Bacillus Cereus ◽  
Phospholipase C

Effect of Phospholipase C on Human Platelets

1975 ◽  
Author(s):  
A.-B. Otnœss
Keyword(s):  
Bacillus Cereus ◽  
Phospholipase C ◽  
Electron Micrographs ◽  
Gel Filtration ◽  
Outer Part ◽  
Human Platelets ◽  
Scanning Electron Micrographs ◽  
Platelet Factor ◽  
Asymmetrical Distribution ◽  
Scanning Electron

The effect on human platelets of phospholipase C (Bacillus cereus) has been studied. Platelets prepared by gel filtration lost 20-30% of their phospholipids when incubated with phospholipase C for 20 min. Phosphatidylethanolamine (PE) was reduced by about 50%, whereas phosphatidylcholine and phosphatidylserine were reduced each by about 20%. Sphingomyelin was not reduced.These data suggest an asymmetrical distribution of phospholipids in the platelet membrane, PE being more accessible and therefore probably mainly located in the outer part of the membrane.The loss of phospholipids was not accompanied by aggregation, nor did the platelets lose their ability to aggregate with thrombin or ADP. Data on release of serotonin, platelet factor 3 and 4 and scanning electron micrographs of treated platelets will be given.

The binding and hydrolysis of sphingomyelin by phospholipase C (Bacillus cereus) as shown by 31P NMR

1984 ◽  
Vol 16 (8) ◽  
pp. 931-934 ◽  
Author(s):  
Kjf.ll M. Aalmo ◽  
Jostein Krane ◽  
Clive Little ◽  
Carlyle B. Storm
Keyword(s):  
Bacillus Cereus ◽  
Phospholipase C ◽  
31P Nmr ◽  
Hydrolysis Of
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