scholarly journals Cloning and nucleotide sequence of a heat-stable amylase gene from an anaerobic thermophile, Dictyoglomus thermophilum

1988 ◽  
Vol 174 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Shoji FUKUSUMI ◽  
Akito KAMIZONO ◽  
Sueharu HORINOUCHI ◽  
Teruhiko BEPPU
Keyword(s):  
Nucleotide Sequence ◽  
Amylase Gene ◽  
Heat Stable ◽  
Dictyoglomus Thermophilum

scholarly journals Cloning, Nucleotide Sequence, and Expression of the Gene Encoding the Bacteriocin Boticin B from Clostridium botulinum Strain 213B

2000 ◽  
Vol 66 (12) ◽  
pp. 5480-5483 ◽  
Author(s):  
Sean S. Dineen ◽  
Marite Bradshaw ◽  
Eric A. Johnson
Keyword(s):  
Amino Acids ◽  
Nucleotide Sequence ◽  
Clostridium Botulinum ◽  
Shuttle Vector ◽  
Open Reading Frame ◽  
Gene Encoding ◽  
Reading Frame ◽  
Heat Stable ◽  
Group I

ABSTRACT Boticin B is a heat-stable bacteriocin produced byClostridium botulinum strain 213B that has inhibitory activity against various strains of C. botulinum and related clostridia. The gene encoding the bacteriocin was localized to a 3.0-kb HindIII fragment of an 18.8-kb plasmid, cloned, and sequenced. DNA sequencing revealed the boticin B structural gene,btcB, to be an open reading frame encoding 50 amino acids. A C. botulinum strain 62A transconjugant containing theHindIII fragment inserted into a clostridial shuttle vector expressed boticin B, although at much lower levels than those observed in C. botulinum 213B. To our knowledge, this is the first demonstration and characterization of a bacteriocin from toxigenic group I C. botulinum.

scholarly journals Nucleotide Sequence of the [alpha]-Amylase Gene from Vigna mungo

1993 ◽  
Vol 103 (4) ◽  
pp. 1459-1459 ◽  
Author(s):  
H. Takeuchi ◽  
D. Yamauchi ◽  
S. Wada ◽  
T. Minamikawa
Keyword(s):  
Nucleotide Sequence ◽  
Vigna Mungo ◽  
Alpha Amylase ◽  
Amylase Gene ◽  
Alpha Amylase Gene

scholarly journals Heat-stable amylase complex produced by a strictly anaerobic and extremely thermophilic bacterium, Dictyoglomus thermophilum.

1988 ◽  
Vol 52 (2) ◽  
pp. 615-616 ◽  
Author(s):  
Yasuhiko KOBAYASHI ◽  
Mahoko MOTOIKE ◽  
Shoji FUKUZUMI ◽  
Toyoji OHSHIMA ◽  
Takashi SAIKI ◽  
...  
Keyword(s):  
Thermophilic Bacterium ◽  
Strictly Anaerobic ◽  
Heat Stable ◽  
Dictyoglomus Thermophilum
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