Improvement of the insecticidal activity of Bacillus thuringiensis var. entomocidus on larvae of Spodoptera littoralis (Lepidoptera, Noctuidae) by addition of chitinolytic bacteria, a phagostimulant and a UV-protectant

2009 ◽  
Vol 96 (1-5) ◽  
pp. 77-83 ◽  
Author(s):  
B. Sneh ◽  
Silvia Schuster ◽  
S. Gross
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Spodoptera Littoralis ◽  
Chitinolytic Bacteria ◽  
Lepidoptera Noctuidae

scholarly journals Combinational Effect of Rumex tingitanus (Polygonaceae) Hexane Extract and Bacillus thuringiensis  δ-Endotoxin against Spodoptera littoralis (Lepidoptera: Noctuidae)

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Dhekra Mhalla ◽  
Dalel Ben Farhat-Touzri ◽  
Slim Tounsi ◽  
Mohamed Trigui
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Bacillus Thuringiensis ◽  
Synergistic Effect ◽  
Spodoptera Littoralis ◽  
Insect Pests ◽  
Hexane Extract ◽  
Active Fraction ◽  
Lepidoptera Noctuidae ◽  
Potential Use

The increasing insect resistance against Bacillus thuringiensis delta-endotoxins is a serious problem which makes it urgent to look for new eco-friendly strategies. Combining these toxins with other biomolecules is one of the promising strategies against insect pests. In this work, we evaluated the bioinsecticidal potential of Rumex tingitanus extracts and B. thuringiensis strain BLB250 against Spodoptera littoralis (Lepidoptera: Noctuidae) larvae. The chemical composition of the hexane extract, the most active fraction, was analyzed to validate the correlation between chemical composition and biological activity. Among the tested extracts, only the hexanic extract showed toxicity against first and second instar larvae with LC50 of 2.56 and 2.95 mg g−1, respectively. The Bacillus thuringiensis BLB250 delta-endotoxins showed toxicity with an LC50 of 56.3 μg g−1. Therefore, the investigated combinational effect of BLB250 delta-endotoxins and R. tingitanus hexane extract proved significant synergistic effect against S. littoralis larvae. The GC-MS analysis of R. tingitanus hexane extract showed the richness of this extract in phytosterols such as β and γ-sitosterol (48.91%), campesterol (6.43%), and β-amyrin (8.92%) which are known for their insecticidal activity. This novel finding highlights the potential use of this combination against insect pests to prevent the appearance of resistance problems.

Inheritance of Resistance to the Bacillus thuringiensis CrylC Toxin in Spodoptera littoralis (Lepidoptera: Noctuidae)

1997 ◽  
Vol 90 (4) ◽  
pp. 873-878 ◽  
Author(s):  
Josette Chaufaux ◽  
Judy MÛller-Cohn ◽  
Christophe Buisson ◽  
Vincent Sanchis ◽  
Didier Lereclus ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Littoralis ◽  
Inheritance Of Resistance ◽  
Lepidoptera Noctuidae

Survival Rates of Tobacco Budworm (Lepidoptera: Noctuidae) Larvae Exposed to Transgenic Cottons Expressing Insecticidal Protein of Bacillus thuringiensis Berliner

2000 ◽  
Vol 35 (2) ◽  
pp. 105-117 ◽  
Author(s):  
C. D. Parker ◽  
V. J. Mascarenhas ◽  
R. G. Luttrell ◽  
K. Knighten
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Heliothis Virescens ◽  
Survival Rates ◽  
Larval Survival ◽  
Tobacco Budworm ◽  
Bt Cotton ◽  
Plant Structure ◽  
Lepidoptera Noctuidae ◽  
Insecticidal Plants

The insecticidal activity of transgenic cottons expressing endotoxin protein of Bacillus thuringiensis Berliner (Bt cotton) was quantified by measuring survival of tobacco budworm, Heliothis virescens (F.), larvae caged on different plant structures for varying lengths of exposure. Percentages of larvae surviving were measured on Bt cottons expressing Cry1Ab and CrylAc protein. Plant structure (terminal, leaf, square or boll) did not affect larval survival, and survival did not differ significantly between CrylAb and CrylAc cottons. Larvae exposed to Bt cotton for only 24 h had higher initial survival than larvae exposed for 48, 72 and 96 h. Larvae first exposed to Bt cotton at 4 d of age had higher survival than those first exposed as neonate or 2-d-old larvae. Survivorship of neonate and 4-d-old larvae exposed to CrylAc cotton was significantly reduced with only 48 h of exposure to the insecticidal plants. Seven-day-old larvae exhibited no significant reduction in survivorship with exposure to CrylAc cotton for 48 h.

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