EFFECT OF ACTINOMYCIN-D ON LABELLED MATERIAL IN THE RETINA AND OPTIC TECTUM OF GOLDFISH AFTER INTRAOCULAR INJECTION OF TRITIATED RNA PRECURSORS

1974 ◽  
Vol 23 (4) ◽  
pp. 681-687 ◽  
Author(s):  
N. A. Ingoglia ◽  
Bernice Grafstein ◽  
B. S. McEwen
Keyword(s):  
Optic Tectum ◽  
Actinomycin D ◽  
Intraocular Injection

Effect of light on the labeling of optic tectum gangliosides after an intraocular injection of N-[3H]acetylmannosamine

1979 ◽  
Vol 86 (3) ◽  
pp. 849-854 ◽  
Author(s):  
Beatriz L. Caputto ◽  
Alicia H.R. Maccioni ◽  
Carlos A. Landa ◽  
Ranwel Caputto
Keyword(s):  
Optic Tectum ◽  
Intraocular Injection ◽  
Effect Of Light

Extracellular proteins of goldfish optic tectum labeled by intraocular injection of 3H-proline

1992 ◽  
Vol 117 (3) ◽  
pp. 260-268 ◽  
Author(s):  
Finnbogi R. Thormodsson ◽  
Edna Antonian ◽  
Bernice Grafstein
Keyword(s):  
Optic Tectum ◽  
Extracellular Proteins ◽  
Intraocular Injection

Retinal projections in quail (Coturnix coturnix)

1989 ◽  
Vol 3 (4) ◽  
pp. 377-387 ◽  
Author(s):  
Robert B. Norren ◽  
Rae Silver
Keyword(s):  
Optic Nerve ◽  
Horseradish Peroxidase ◽  
Optic Tectum ◽  
Lateral Part ◽  
Retinal Projections ◽  
Coturnix Coturnix ◽  
Intraocular Injection ◽  
Anterior Thalamus ◽  
Ventrolateral Thalamus ◽  
Pretectal Area

AbstractThe trajectory of retinal projections and the location of retinorecipient nuclei in the quail brain was examined after application of horseradish peroxidase (HRP) either to the cut end of the optic nerve or following intraocular injection of HRP. Retinal projections to the hypothalamus, dorsalateral anterior thalamus (rostralateral part, magnocellular part, and lateral part), lateral anterior thalamus, lateroventral geniculate nucleus, lateral geniculate intercalated nuclei (rostral and caudal parts), ventrolateral thalamus, superficial synencephalic nucleus, external nucleus, tectal gray, diffuse pretectal area, pretectal optic area, ectomammillary nucleus, and optic tectum were revealed. Retinal projections observed in quail were compared with results obtained in other avian species and considered in relation to possible anatomic pathways underlying photoperiodism and circadian rhythms.

Radioautography of the Optic Tectum of the Goldfish after Intraocular Injection of [3H]Proline

Science ◽  
1972 ◽  
Vol 176 (4033) ◽  
pp. 407-410 ◽  
Author(s):  
J. H. Neale ◽  
E. A. Neale ◽  
B. W. Agranoff
Keyword(s):  
Optic Tectum ◽  
Intraocular Injection

Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

1971 ◽  
Vol 29 ◽  
pp. 548-549
Author(s):  
Awtar Krishan ◽  
Dora Hsu
Keyword(s):  
Granular Material ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Actinomycin D ◽  
Metabolic Inhibitors ◽  
Protein And Rna Synthesis ◽  
Apparent Reduction ◽  
Plant Alkaloids ◽  
In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

ANALYSIS OF THE BIPHASIC ACTION OF GROWTH HORMONE IN VITRO ON THE RAT DIAPHRAGM

1968 ◽  
Vol 57 (3_Suppl) ◽  
pp. S19-S35 ◽  
Author(s):  
Å. Hjalmarson
Keyword(s):  
Growth Hormone ◽  
Actinomycin D ◽  
Accumulation Rate ◽  
Inhibitory Effect ◽  
Bovine Growth Hormone ◽  
Rat Diaphragm ◽  
Dual Nature ◽  
Hypophysectomized Rats ◽  
D 20

ABSTRACT In vitro addition of bovine growth hormone (GH) to intact hemidiaphragms from hypophysectomized rats has previously been found to produce both an early stimulatory effect lasting for 2—3 hours and a subsequent late inhibitory effect during which the muscle is insensitive to further addition of GH (Hjalmarson 1968). These effects on the accumulation rate of α-aminoisobutyric acid (AIB) and D-xylose have been further studied. In presence of actinomycin D (20 μg/ml) or puromycin (100 μg/ml) the duration of the stimulatory effect of GH (25 μg/ml) was prolonged to last for at least 4—5 hours and the late inhibitory effect was prevented. Similar results were obtained when glucose-free incubation medium was used. Preincubation of the diaphragm at different glucose concentrations (0—5 mg/ml) for 3 hours did not change the GH sensitivity. Addition of insulin at start of incubation could not prevent GH from inducing its late inhibitory effect, while dexamethasone seemed to potentiate this effect of GH. Furthermore, adrenaline was found to decrease the uptake of AIB-14C and D-xylose-14C in the diaphragm, but not to change the sensitivity of the muscle to GH. Preincubation of the diaphragm for 3 hours with puromycin in a concentration of 200 μg/ml markedly decreased the subsequent basal uptake of both AIB-14C and D-xylose-14C, in the presence of puromycin, and abolished the stimulatory effect of GH on the accumulation of AIB-14C. However, the effect of GH on the accumulation of D-xylose-14C was unchanged. The present observations are discussed and evaluated in relation to various mechanisms of GH action proposed to explain the dual nature of the hormone.

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