Evidence for Specific Polypeptide Chain Folding in Myelin Basic Protein from Reactions Between Fragments of the Protein and Monoclonal Antibodies

Carbon-13 n.m.r, spectra have been obtained for bovine myelin basic protein at pD 4.4 in D2O and in 6 M guanidine deuterochloride solutions. Chemical-shift differences between resonances from some amino acid residues are interpreted in terms of structured regions in the polypeptide chain of the native protein, whereas the denatured protein displays the spectrum expected for an essentially random coil. Measurements of T1 and n.O.e. provide quantitative data on the dynamics of the backbone and side-chain carbons, and give support to the conclusion that the native protein does not have a random-coil structure.

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Preparation and properties of monoclonal antibodies to myelin basic protein and its peptides

Neurochemistry International ◽

10.1016/0197-0186(85)90120-2 ◽

1985 ◽

Vol 7 (2) ◽

pp. 309-317 ◽

Cited By ~ 25

Author(s):

Nigel Groome ◽

Janice Harland ◽

Adrian Dawkes

Keyword(s):

Monoclonal Antibodies ◽

Myelin Basic Protein ◽

Basic Protein

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Expression of myelin basic protein (MBP) epitopes in human non-neural cells revealed by two anti-MBP IgM monoclonal antibodies

Clinical & Experimental Immunology ◽

10.1046/j.1365-2249.2000.01363.x ◽

2000 ◽

Vol 122 (3) ◽

pp. 429-436 ◽

Cited By ~ 6

Author(s):

R. Chignola ◽

T. Cestari ◽

C. Guerriero ◽

A. P. Riviera ◽

S. Ferrari ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Myelin Basic Protein ◽

Basic Protein ◽

Neural Cells

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Monoclonal Antibodies to Myelin Basic Protein

Protides of the Biological Fluids ◽

10.1016/b978-0-08-029815-3.50119-7 ◽

1983 ◽

pp. 525-528 ◽

Cited By ~ 1

Author(s):

L.R. SIRES ◽

S. HRUBY ◽

E.C. ALVORD

Keyword(s):

Monoclonal Antibodies ◽

Myelin Basic Protein ◽

Basic Protein

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MONOCLONAL ANTIBODIES AGAINST MYELIN BASIC PROTEIN

Journal of Neuropathology & Experimental Neurology ◽

10.1097/00005072-198205000-00051 ◽

1982 ◽

Vol 41 (3) ◽

pp. 355 ◽

Cited By ~ 1

Author(s):

W. F. Hickey ◽

L. J. McMillan ◽

T. J. McKearn ◽

J. Gonatas ◽

N. K. Gonatas

Keyword(s):

Monoclonal Antibodies ◽

Myelin Basic Protein ◽

Basic Protein

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Immunohistochemical application of monoclonal antibodies against myelin basic protein and neurofilament triple protein subunits: advantages over antisera and technical limitations.

Journal of Histochemistry & Cytochemistry ◽

10.1177/31.9.6193166 ◽

1983 ◽

Vol 31 (9) ◽

pp. 1126-1135 ◽

Cited By ~ 51

Author(s):

W F Hickey ◽

V Lee ◽

J Q Trojanowski ◽

L J McMillan ◽

T J McKearn ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Human Brain ◽

Myelin Basic Protein ◽

Brain Tissue ◽

Mercuric Chloride ◽

Basic Protein ◽

Histological Study ◽

Cross Reactivity ◽

Human Brain Tissue ◽

Specific Staining

Four monoclonal antibodies against guinea pig myelin basic protein (MBP), and four against subunits of bovine neurofilament triplet proteins (NF) were produced and their activity determined by enzyme-linked immunosorbant assay. The specificity and cross-reactivity of these eight monoclonal antibodies and one heterologous antiserum against each of the two central nervous system (CNS) antigens were examined in a histological study using the immunoperoxidase, antibody sandwich technique in rat and human brain tissue. Tissue sections were prepared from paraffin-embedded or fresh brain tissue that had been fixed with one of five different fixatives. The resulting immunoperoxidase labeling was then graded for intensity and examined for artifacts. One monoclonal antibody against MBP and one against NF resulted in labeling that was superior to that given by each of the antisera against their respective antigens. Of the five fixatives tested, a mercuric chloride-formalin solution gave the best preservation of these two antigens in rat and human brain tissue. The mercuric chloride-formalin solution was found to be superior to the other fixatives when immersion fixation was used, and was especially optimal when brains were perfused fixed. Three artifacts were encountered among the various antibody-fixative combinations that produced erroneous, but seemingly specific staining of Purkinje cells, neurons and axons, or astrocytes.

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