Development, characterization and virus susceptibility of a continuous cell line from the caudal fin of marbled eel (Anguilla marmorata )

2018 ◽  
Vol 41 (9) ◽  
pp. 1331-1338 ◽  
Author(s):  
H Y Pao ◽  
C Y Wu ◽  
C H Huang ◽  
C M Wen

2018 ◽  
Vol 41 (4) ◽  
pp. 635-641 ◽  
Author(s):  
S W Yeh ◽  
Y H Cheng ◽  
F N Nan ◽  
C M Wen


In Vitro ◽  
1980 ◽  
Vol 16 (11) ◽  
pp. 915-917 ◽  
Author(s):  
Goro Kuno






Blood ◽  
1983 ◽  
Vol 62 (5) ◽  
pp. 1132-1134 ◽  
Author(s):  
JL Ascensao ◽  
F Gaylis ◽  
D Bronson ◽  
EE Fraley ◽  
ED Zanjani

Abstract An established human testis germ cell line (1411-H) was found to produce significant amounts of erythropoietin (Ep), the primary regulator of erythropoiesis. Media conditioned by the 1411-H cells stimulated erythropoiesis both in vivo and in vitro. This activity was neutralized by anti-Ep. This continuous cell line provides a unique model for the study of the mechanisms of control of Ep biogenesis.



1998 ◽  
Vol 275 (2) ◽  
pp. G259-G268 ◽  
Author(s):  
Yingting Zhu ◽  
John M. Aletta ◽  
Jiayu Wen ◽  
Xuejun Zhang ◽  
Dennis Higgins ◽  
...  

To establish a continuous cell line, freshly prepared rat parotid acinar cells were stably transfected with a plasmid vector containing the SV40 large T antigen. The acinar origin of these cells was confirmed by Western blotting, enzyme analysis, and morphological analysis. Transformed cells grown in 10% rat serum showed a modest reduction in cell number after 7 days and a concentration- and time-dependent increase in amylase levels ∼16 times greater than those observed in fetal bovine serum-treated cells. Ultrastructural analysis revealed that cells grown in rat serum harbored protein-filled secretory granules localized adjacent to the endoplasmic reticulum, and punctate amylase-specific immunofluorescence distributed throughout the cytoplasm was consistent with the presence of amylase in secretory organelles. Clonal cells express tissue-specific proline-rich proteins and the four protein kinase C isozymes present in primary culture. Carbachol and isoproterenol stimulated [3H]protein secretion and isoproterenol enhanced amylase secretion from cells grown in rat serum. Moreover, norepinephrine, carbachol, and substance P produced a time- and concentration-dependent rise in cytoplasmic Ca2+. This continuous cell line of parotid acinar cells, which after treatment with rat serum retains the basic structural and functional properties of primary culture cells, will be utilized as a model system for studying long-term biological processes that regulate parotid cell function.



1990 ◽  
Vol 8 (6) ◽  
pp. 409-424 ◽  
Author(s):  
Gian Paolo Bagnara ◽  
Marco Giovannini ◽  
Massimo Serra ◽  
Piero Picci ◽  
Mario Campanacci ◽  
...  


2006 ◽  
Vol 42 (8) ◽  
pp. 263 ◽  
Author(s):  
S. J. DEWITTE-ORR ◽  
K. LEPIC ◽  
S. P. BRYSON ◽  
S. K. WALSH ◽  
L. E. J. LEE ◽  
...  


Sign in / Sign up

Export Citation Format

Share Document