Diagnostic performance of HPV E6 / E7 mRNA testing towards HPV ‐ DNA testing and p16 /Ki67 immunostaining as a biomarker of high‐risk HPV recurrence in Greek women surgically treated for their cervical lesions

2021 ◽  
Author(s):  
Fausto Carcea ◽  
Eleftherios Vavoulidis ◽  
Stamatios Petousis ◽  
Panagiotis Papandreou ◽  
Chrysoula Margioula Siarkou ◽  
...  
Keyword(s):  
High Risk ◽  
Diagnostic Performance ◽  
Dna Testing ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Hpv E6 ◽  
Hpv Dna Testing ◽  
High Risk Hpv

The evaluation of human papillomavirus DNA testing in primary screening for cervical lesions in a large Japanese population

2006 ◽  
Vol 16 (3) ◽  
pp. 1007-1013 ◽  
Author(s):  
M. Inoue ◽  
J. Sakaguchi ◽  
T. Sasagawa ◽  
M. Tango
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Single Type ◽  
Dna Testing ◽  
Cervical Lesions ◽  
Papanicolaou Test ◽  
Histologic Diagnosis ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
High Risk Hpv

To examine the utility of human papillomavirus (HPV) DNA testing for the screening of cervical cancer and its precursors, a prospective cohort study was performed in which a total of 8156 women with a median age of 36 years were enrolled. Two smear samples scraped from the uterine cervix were served for Papanicolaou test and HPV DNA testing (Hybrid Capture-II system). HPV-positive samples were further examined for HPV subtype using a DNA microarry chip. Women with cytologic abnormality or those with high-risk HPV DNA were further examined by colposcopy to determine histologic diagnosis. High-risk HPV DNA was detected in 11% of the general population, with higher prevalence of specific types, including 52, 16, 58, 51, 56, and 18. As expected, younger women were likely to have increased frequency of HPV infection. Notably, HPV DNA testing detected all 45 cases of cervical intraepithelial neoplasia (CIN) 3, while cytologic findings were negative in 6 of these cases. It is of particular interest that CIN was commonly associated with multiple HPV types, while invasive cancers had a single type of HPV. In terms of both sensitivity and positive predictive value in detecting the CIN, HPV DNA testing is superior to cytology. However, most importantly, HPV DNA testing in combination with cytology significantly improved the efficacy to CIN screening.

Off-Label High-Risk HPV DNA Testing of Vaginal ASC-US and LSIL Cytologic Abnormalities at Parkland Hospital

2010 ◽  
Vol 14 (4) ◽  
pp. 352-355 ◽  
Author(s):  
Catherine A. Chappell ◽  
Ann Marie West ◽  
Wareef Kabbani ◽  
Claudia L. Werner
Keyword(s):  
High Risk ◽  
Dna Testing ◽  
Hpv Dna ◽  
Off Label ◽  
Hpv Dna Testing ◽  
High Risk Hpv ◽  
Cytologic Abnormalities

scholarly journals Comparison of Human Papillomavirus Detection in Urine and Cervical Samples Using High-Risk HPV DNA Testing in Northern Thailand

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Surapan Khunamornpong ◽  
Jongkolnee Settakorn ◽  
Kornkanok Sukpan ◽  
Suree Lekawanvijit ◽  
Narisara Katruang ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Hpv Testing ◽  
Dna Testing ◽  
Northern Thailand ◽  
Cervical Sample ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Hpv Test ◽  
High Risk Hpv

Objective. To evaluate the performance of high-risk human papillomavirus (HPV) DNA testing in urine samples compared to that of cervical sample testing in Northern Thailand. Methods. Paired urine and cervical samples were collected during the follow-up of women with a previous positive HPV test. HPV testing was performed using the Cobas 4800 HPV Test. Linear Array assay was used for genotyping in selected cases. Results. Paired urine and cervical samples were obtained from 168 women. Of 123 paired samples with valid results, agreement in the detection of high-risk HPV DNA was present in 106 cases (86.2%), with a kappa statistic of 0.65 (substantial agreement). Using the cervical HPV results as a reference, the sensitivity of urine HPV testing was 68.6% (24/35) and the specificity 93.2% (82/88). For the detection of histologic high-grade squamous intraepithelial lesion or worse (HSIL+), the sensitivity of urine HPV testing was 80.0% (4/5) and the specificity 78.0% (92/118). Conclusion. Although urine HPV testing had a rather low sensitivity for HPV detection, its sensitivity for histologic HSIL+ detection was high. For clinical use of urine HPV testing, standardization of specimen collection and processing techniques or application of a more sensitive test, especially in the detection of HPV52 and HPV58, is necessary.

scholarly journals Characteristics of Apparently False-Negative Digene Hybrid Capture 2 High-Risk HPV DNA Testing

2006 ◽  
Vol 125 (2) ◽  
pp. 223-228 ◽  
Author(s):  
Raid Jastania ◽  
William R. Geddie ◽  
William Chapman ◽  
Scott Boerner
Keyword(s):  
High Risk ◽  
False Negative ◽  
Dna Testing ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Hybrid Capture 2 ◽  
High Risk Hpv

The role of high-risk HPV-DNA testing in the male sexual partners of women with HPV-induced lesions

2008 ◽  
Vol 137 (1) ◽  
pp. 88-91 ◽  
Author(s):  
Paulo C. Giraldo ◽  
Jose Eleutério ◽  
Diane Isabelle M. Cavalcante ◽  
Ana Katherine S. Gonçalves ◽  
Juliana A.A. Romão ◽  
...  
Keyword(s):  
High Risk ◽  
Sexual Partners ◽  
Dna Testing ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Male Sexual Partners ◽  
High Risk Hpv

p16/Ki-67 Immunocytochemistry in Gynecological Cytology: Limitations in Practice

2017 ◽  
Vol 61 (3) ◽  
pp. 230-236 ◽  
Author(s):  
Peter Ziemke
Keyword(s):  
Risk Assessment ◽  
High Risk ◽  
Positive Result ◽  
Dna Testing ◽  
Ki 67 ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
Immunochemical Detection ◽  
High Risk Hpv

Objective: Immunochemical detection of the protein p16INK4a in cervical cytology is used in combination with Ki-67. Cells positive for both proteins are certain to have been transformed by high-risk HPV. p16/Ki-67 immunocytochemistry provides a significant improvement in specificity over cytology and HPV DNA testing. However, p16/Ki-67 immunocytochemistry also has its limitations. Study Design: The research is based on the follow-up of 1,131 patients for whom p16/Ki-67 immunocytochemistry was performed with cytology. Dependencies on the age of patients with LSIL, number of p16/Ki-67-positive cells, and different results during repeated examinations were analyzed. Results: In LSIL, positive p16/Ki-67 is less specific for ≥CIN2/HSIL for patients younger than 30 years compared to patients aged 30 years or older (61.1 vs. 75.7%, p < 0.013). Using a score of 10 p16/Ki-67-marked cells as a positive result instead of 1 led to significantly higher specificity (89.0 vs. 70.2%, p < 0.001). This modified threshold offers better risk assessment in LSIL. In repeated immunocytochemical investigations, 28.4% of the results deviated from the first examination. Conclusion: The abovementioned discrepancies can be interpreted as hints about the molecular biological causes of suboptimal performance of p16/Ki-67. An efficient and reliable application of p16/Ki-67 immunocytochemistry requires knowledge of its methodological limitations.

Value of high-risk HPV-DNA testing in the triage of ASCUS

2009 ◽  
Vol 88 (9) ◽  
pp. 1006-1010 ◽  
Author(s):  
Iréne Silverloo ◽  
Bengt Andrae ◽  
Erik Wilander
Keyword(s):  
High Risk ◽  
Dna Testing ◽  
Hpv Dna ◽  
Hpv Dna Testing ◽  
High Risk Hpv
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