Development and Characterization of Monoclonal Antibodies for the Detection of Fish Protein

This study developed and characterized anti-fish monoclonal antibodies (mAbs) capable of detecting fish, a major allergenic food, in processed food products to protect fish sensitized individuals. Of the three mAbs raised against crude protein extract of cooked fish muscle, mAb 8F5 exhibited a positive reaction to all 50 common food fish species tested with no cross-reactions to shellfish, land animals, or food additives. Although the ELISA results were negative against swordfish and yellowfin tuna, western blot clearly detected both after cooking. The ~36 kDa antigenic protein of mAb 8F5, which was found in all fish species, was detectable by mAb 8F5 in all of the fish samples even after prolonged heat treatment (100 °C, up to 60 min). These findings suggest that mAb 8F5 has great potential utility as a probe for the immunochemical detection of fish tissue in cooked food.

Rapid detection of mozzarella and feta cheese adulteration with cow milk through a silicon photonic immunosensor

Immunochemical detection of Mozzarella di Bufala Campana and Feta cheese adulteration with cow milk using integrated silicon Mach–Zehnder interferometers.

Quantitative Analysis of Fecal Hemoglobin Concentration in Colorectal Cancer Patients – The Values in the Screening and Diagnosis of Colorectal Cancer

Objective: To use immunochemical detection of fecal hemoglobin amount as a technical method, to perform a FIT test on the fecal specimens of research subjects who were going to undergo colonoscopy. The recorded results are compared and analyzed, and the sensitivity and specificity of the detection method in the diagnosis of colorectal cancer were evaluated. Methods: Three hundreds of individuals who were going to undergo colonoscopy were tested for GFOBT and FIT in feces in advance, and 20 ?g/g was used as the threshold for determining positive FIT result. The results were analyzed and compared with the results of colonoscopy. Results: The sensitivity of FIT to the diagnosis of colorectal cancer was 80.95%, and the specificity was 86.54%. The positive predictive values of FIT test in colorectal cancer screening were 53.1% and 81.77%, respectively. Conclusion: FIT is more sensitive and specific for detecting colorectal cancer and adenoma. The fecal occult blood test (FOBT) is stronger, and FIT test detection is more valuable in colon cancer screening.

IMMUNOCHEMICAL DIAGNOSIS OF CANCER. PROTOTYPING

A prototype of the method of immunochemical detection of different types of solid cancers (primary and recurrent) in the early stages was developed. According to the initial hypothesis, the sera of patients with malignant tumors of different localization and different histological nature, contain different sets of autoantibodies (auto-Ab) of IgG class to many cancer-associated antigens (CA-AG). The content of such auto-Ab differs in cancer patients and patients with non-malignant chronic diseases, which determines the difference in serum immunoreactivity profiles of cancer patients and patients with non-malignant diseases. Confirmation of this hypothesis opens up prospects for the creation of simple and cheap laboratory methods of mass preventive examination of the population for the early detection of different cancers. The confirmation of the hypothesis was obtained. Moreover, even with non-optimal sets of test antigens, with the help of solid-phase ELISA it was possible to achieve sensitivity of 71% and specificity of 68% in the differentiation of blood sera of cancer (lung, stomach, ovary, prostate) and non-cancer patients (chronic inflammatory diseases of the lungs, stomach, ovary, prostate) and nearly 90% in the differentiation of healthy individuals from cancer patients.