scholarly journals Crassulacean acid metabolism guard cell anion channel activity follows transcript abundance and is suppressed by apoplastic malate

2020 ◽  
Vol 227 (6) ◽  
pp. 1847-1857 ◽  
Author(s):  
Cécile Lefoulon ◽  
Susanna F. Boxall ◽  
James Hartwell ◽  
Michael R. Blatt
Keyword(s):  
Guard Cell ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Anion Channel ◽  
Transcript Abundance ◽  
Channel Activity

scholarly journals Transcriptional profiles of organellar metabolite transporters during induction of crassulacean acid metabolism in Mesembryanthemum crystallinum

2005 ◽  
Vol 32 (5) ◽  
pp. 451 ◽  
Author(s):  
Shin Kore-eda ◽  
Chiyuki Noake ◽  
Masahisa Ohishi ◽  
Jun-ichi Ohnishi ◽  
John C. Cushman
Keyword(s):  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Expression Patterns ◽  
Transcript Abundance ◽  
Transport Processes ◽  
Mesembryanthemum Crystallinum ◽  
Water Deficit Stress ◽  
Diurnal Changes ◽  
Metabolite Transport ◽  
Leaf Tissues

Metabolite transport across multiple organellar compartments is essential for the operation of crassulacean acid metabolism (CAM). To investigate potential circadian regulation of inter-organellar metabolite transport processes, we have identified eight full-length cDNAs encoding an organellar triose phosphate / Pi translocator (McTPT1), a phosphoenolpyruvate / Pi translocator (McPPT1), two glucose-6-phosphate / Pi translocators (McGPT1, 2), two plastidic Pi translocator-like proteins (McPTL1, 2), two adenylate transporters (McANT1, 2), a dicarboxylate transporter (McDCT2), and a partial cDNA encoding a second dicarboxylate transporter (McDCT1) in the model CAM plant, Mesembryanthemum crystallinum L. We next investigated day / night changes in steady-state transcript abundance of each of these transporters in plants performing either C3 photosynthesis or CAM induced by salinity or water-deficit stress. We observed that the expression of both isogenes of the glucose-6-phosphate / Pi translocator (McGPT1, 2) was enhanced by CAM induction, with McGPT2 transcripts exhibiting much more pronounced diurnal changes in transcript abundance than McGPT1. Transcripts for McTPT1, McPPT1, and McDCT1 also exhibited more pronounced diurnal changes in abundance in the CAM mode relative to the C3 mode. McGPT2 and McDCT1 transcripts exhibited sustained oscillations for at least 3 d under constant light and temperature conditions suggesting their expression is under circadian clock control. McTPT1 and McGPT2 transcripts were preferentially expressed in leaf tissues in either C3 or CAM modes. The leaf-specific and / or circadian controlled gene expression patterns are consistent with McTPT1, McGPT2 and McDCT1 playing CAM-specific metabolite transport roles.

scholarly journals Kalanchoë PPC1 Is Essential for Crassulacean Acid Metabolism and the Regulation of Core Circadian Clock and Guard Cell Signaling Genes

2020 ◽  
Vol 32 (4) ◽  
pp. 1136-1160 ◽  
Author(s):  
Susanna F. Boxall ◽  
Nirja Kadu ◽  
Louisa V. Dever ◽  
Jana Kneřová ◽  
Jade L. Waller ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Circadian Clock ◽  
Guard Cell ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism

scholarly journals Silencing PHOSPHOENOLPYRUVATE CARBOXYLASE1 in the Obligate Crassulacean Acid Metabolism Species Kalanchoë laxiflora causes Reversion to C3-like Metabolism and Amplifies Rhythmicity in a Subset of Core Circadian Clock Genes

2019 ◽  
Author(s):  
Susanna F. Boxall ◽  
Nirja Kadu ◽  
Louisa V. Dever ◽  
Jana Kneřová ◽  
Jade L. Waller ◽  
...  
Keyword(s):  
Guard Cell ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Clock Genes ◽  
Dark Period ◽  
Stomatal Closure ◽  
Delayed Fluorescence ◽  
Light Period ◽  
Cam Plants ◽  
Circadian Clock Genes

ABSTRACTUnlike C3 plants, Crassulacean acid metabolism (CAM) plants fix CO2 in the dark using phosphoenolpyruvate carboxylase (PPC; EC 4.1.1.31). PPC combines PEP with CO2 (as HCO3−), forming oxaloacetate that is rapidly converted to malate, leading to vacuolar malic acid accumulation that peaks phased to dawn. In the light period, malate decarboxylation concentrates CO2 around RuBisCO for secondary fixation. CAM mutants lacking PPC have not been described. Here, RNAi was employed to silence CAM isogene PPC1 in Kalanchoë laxiflora. Line rPPC1-B lacked PPC1 transcripts, PPC activity, dark period CO2 fixation, and nocturnal malate accumulation. Light period stomatal closure was also perturbed, and the plants displayed reduced but detectable dark period stomatal conductance, and arrhythmia of the CAM CO2 fixation circadian rhythm under constant light and temperature (LL) free-running conditions. By contrast, the rhythm of delayed fluorescence was enhanced in plants lacking PPC1. Furthermore, a subset of gene transcripts within the central circadian oscillator were up-regulated and oscillated robustly. The regulation guard cell genes involved controlling stomatal movements was also altered in rPPC1-B. This provided direct evidence that altered regulatory patterns of key guard cell signaling genes are linked with the characteristic inverse pattern of stomatal opening and closing during CAM.

scholarly journals Does the C4 plant Trianthema portulacastrum (Aizoaceae) exhibit weakly expressed crassulacean acid metabolism (CAM)?

2020 ◽  
Author(s):  
Klaus Winter ◽  
Milton Garcia ◽  
Aurelio Virgo ◽  
Jorge Ceballos ◽  
Joseph A. M. Holtum
Keyword(s):  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
C4 Plant ◽  
Trianthema Portulacastrum

scholarly journals Carbon Isotope Ratios in Crassulacean Acid Metabolism Plants

1976 ◽  
Vol 58 (3) ◽  
pp. 367-370 ◽  
Author(s):  
Stan R. Szarek ◽  
John H. Troughton
Keyword(s):  
Carbon Isotope ◽  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Isotope Ratios ◽  
Carbon Isotope Ratios

Historical aspects and net CO2 uptake for cultivated Crassulacean acid metabolism plants in Mexico

2002 ◽  
Vol 140 (2) ◽  
pp. 133-142 ◽  
Author(s):  
PARK S NOBEL ◽  
EULOGIO PIMIENTA-BARRIOS ◽  
JULIA ZANUDO HERNANDEZ ◽  
BLANCA C RAMIREZ-HERNANDEZ
Keyword(s):  
Crassulacean Acid Metabolism ◽  
Acid Metabolism ◽  
Co2 Uptake ◽  
Historical Aspects ◽  
Net Co2 Uptake
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