Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

2020 ◽  
Vol 55 (6) ◽  
pp. 659-676 ◽  
Author(s):  
Luis B. Ferré ◽  
Michael E. Kjelland ◽  
Ahmed M. Taiyeb ◽  
Fernando Campos‐Chillon ◽  
Pablo J. Ross
Keyword(s):  
In Vitro Culture ◽  
Recent Progress ◽  
Culture Systems

Recent Progress in Prediction Systems for Drug-induced Liver Injury Using in vitro Cell Culture

2020 ◽  
Vol 14 ◽  
Author(s):  
Shogo Ozawa ◽  
Toshitaka Miura ◽  
Jun Terashima ◽  
Wataru Habano ◽  
Seiichi Ishida
Keyword(s):  
Cell Culture ◽  
Recent Progress ◽  
Inflammatory Cells ◽  
Protein Adducts ◽  
In Vitro Assays ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Culture Systems ◽  
Cell Culture Systems

Background: In order to avoid drug-induced liver injury (DILI), in vitro assays, which enable the assessment of both metabolic activation and immune reaction processes that ultimately result in DILI, are needed. Objective: In this study, the recent progress in the application of in vitro assays using cell culture systems is reviewed for potential DILI-causing drugs/xenobiotics and a mechanistic study on DILI, as well as for the limitations of in vitro cell culture systems for DILI research. Methods: Information related to DILI was collected through a literature search of the PubMed database. Results: The initial biological event for the onset of DILI is the formation of cellular protein adducts after drugs have been metabolically activated by drug metabolizing enzymes. The damaged peptides derived from protein adducts lead to the activation of CD4+ helper T lymphocytes and recognition by CD8+ cytotoxic T lymphocytes, which destroy hepatocytes through immunological reactions. Because DILI is a major cause of drug attrition and drug withdrawal, numerous in vitro systems consisting of hepatocytes and immune/inflammatory cells, or spheroids of human primary hepatocytes containing non-parenchymal cells have been developed. These cellular-based systems have identified DILIinducing drugs with approximately 50% sensitivity and 90% specificity. Conclusion: Different co-culture systems consisting of human hepatocyte-derived cells and other immune/inflammatory cells have enabled the identification of DILI-causing drugs and of the actual mechanisms of action.

Trypanosoma cruzi infectivity assessment in “in vitro” culture systems by automated cell counting

Acta Tropica ◽  
2015 ◽  
Vol 143 ◽  
pp. 47-50 ◽  
Author(s):  
Ana Liempi ◽  
Christian Castillo ◽  
Mauricio Cerda ◽  
Daniel Droguett ◽  
Juan Duaso ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
In Vitro Culture ◽  
Cell Counting ◽  
Culture Systems ◽  
Automated Cell Counting

Applications of in vitro culture systems for commercial sugarcane production and improvement

2011 ◽  
Vol 47 (2) ◽  
pp. 234-249 ◽  
Author(s):  
Sandy J. Snyman ◽  
Gwethlyn M. Meyer ◽  
Aimee C. Koch ◽  
Marzena Banasiak ◽  
M. Paula Watt
Keyword(s):  
In Vitro Culture ◽  
Sugarcane Production ◽  
Culture Systems

scholarly journals Evaluation of in vitro culture systems for the maintenance of microfilariae and infective larvae of Loa loa

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Denis Zofou ◽  
Fanny Fri Fombad ◽  
Narcisse V. T. Gandjui ◽  
Abdel Jelil Njouendou ◽  
Arnaud Jonas Kengne-Ouafo ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Loa Loa ◽  
Culture Systems ◽  
Infective Larvae

Use of conditioned medium for IVM-IVF bovine embryos in-vitro culture systems

1993 ◽  
Vol 39 (1) ◽  
pp. 236 ◽  
Author(s):  
W.G. van Inzen ◽  
Th.A.M. Kruip ◽  
S.M. Weima
Keyword(s):  
In Vitro Culture ◽  
Conditioned Medium ◽  
Bovine Embryos ◽  
Culture Systems

Studies on the accumulation of phenolic acids and flavonoids in different in vitro culture systems of Schisandra chinensis (Turcz.) Baill. using a DAD-HPLC method

2017 ◽  
Vol 20 ◽  
pp. 462-469 ◽  
Author(s):  
Agnieszka Szopa ◽  
Adam Kokotkiewicz ◽  
Marcelina Bednarz ◽  
Maria Luczkiewicz ◽  
Halina Ekiert
Keyword(s):  
In Vitro Culture ◽  
Phenolic Acids ◽  
Hplc Method ◽  
Schisandra Chinensis ◽  
Culture Systems
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