A novelHLA-A*02allele,A*02:543was identified by polymerase chain reaction sequence-based typing in a Chinese cord blood donor

HLA ◽  
2016 ◽  
Vol 87 (5) ◽  
pp. 384-385 ◽  
Author(s):  
L.-N. Dong ◽  
N.-Y. Chen ◽  
W. Zhang ◽  
J. He ◽  
F.-M. Zhu
Keyword(s):  
Polymerase Chain Reaction ◽  
Cord Blood ◽  
Blood Donor ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

Identification of the novel HLA-B*40:01:41 allele by polymerase chain reaction sequence-based typing in a Chinese cord blood donor

HLA ◽  
2017 ◽  
Vol 90 (2) ◽  
pp. 118-120 ◽  
Author(s):  
N.-Y. Chen ◽  
W. Wang ◽  
L.-N. Dong ◽  
J. He ◽  
F.-M. Zhu
Keyword(s):  
Polymerase Chain Reaction ◽  
Cord Blood ◽  
Blood Donor ◽  
The Novel ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

Identification of the novelHLA-B*13:83allele by polymerase chain reaction sequence-based typing in a Chinese cord blood donor

HLA ◽  
2017 ◽  
Vol 89 (4) ◽  
pp. 241-242 ◽  
Author(s):  
J. He ◽  
N.-Y. Chen ◽  
Z.-D. Han ◽  
W. Zhang ◽  
F.-M. Zhu
Keyword(s):  
Polymerase Chain Reaction ◽  
Cord Blood ◽  
Blood Donor ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

Identification of the novelHLA-DRB1*08:69allele by polymerase chain reaction sequence-based typing in a Chinese cord blood donor

HLA ◽  
2016 ◽  
Vol 89 (1) ◽  
pp. 64-65 ◽  
Author(s):  
L.-N. Dong ◽  
Z.-D. Han ◽  
W. Zhang ◽  
J. He ◽  
F.-M. Zhu
Keyword(s):  
Polymerase Chain Reaction ◽  
Cord Blood ◽  
Blood Donor ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

Identification of a novelHLA-A*33:03:11allele by polymerase chain reaction sequence-based typing in a Chinese cord blood donor

2013 ◽  
Vol 82 (1) ◽  
pp. 59-60 ◽  
Author(s):  
W. Zhang ◽  
S.-D. Tao ◽  
F.-M. Zhu ◽  
J. He ◽  
H.-J. Lv
Keyword(s):  
Polymerase Chain Reaction ◽  
Cord Blood ◽  
Blood Donor ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

HLA-B*4088, a new allele was identified by polymerase chain reaction sequence-based typing in a Chinese cord blood donor

2010 ◽  
Vol 75 (1) ◽  
pp. 79-80 ◽  
Author(s):  
L.-X. Yan ◽  
F.-M. Zhu ◽  
W. Zhang ◽  
J. He
Keyword(s):  
Polymerase Chain Reaction ◽  
Cord Blood ◽  
Blood Donor ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

Luminex® xMAP® technology is an effective strategy for high-definition human leukocyte antigen typing of cord blood units prior to listing

2018 ◽  
Vol 41 (5) ◽  
pp. 284-288
Author(s):  
Marco Guarene ◽  
Carla Badulli ◽  
Anna L Cremaschi ◽  
Ilaria Sbarsi ◽  
Rosalia Cacciatore ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cord Blood ◽  
Cord Blood Transplantation ◽  
Hla Typing ◽  
High Definition ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Effective Manner ◽  
Polymerase Chain ◽  
The Impact

Introduction: Allele-level donor–recipient match at HLA-A, HLA-B, HLA-C and HLA-DRB1 loci impacts the outcome after cord blood transplantation for hematologic malignancies and modifies the strategy of donor selection. High definition of both class I and II HLA loci at time of listing is a way to improve the attractiveness of cord blood bank inventories, reducing the time for donor search and procurement and simplifying donor choice, in particular, for patients of non-European heritage. Methods: In 2014, Luminex® xMAP® technology was introduced in our laboratory practice and was applied to cord blood units typing. In this study, we evaluated the impact of this strategy in comparison with the platform in use until 2013, relying on LiPA reverse polymerase chain reaction–sequence-specific oligonucleotide (revPCR-SSO) plus polymerase chain reaction–sequence-specific primer (PCR-SSP). Results: In 2014, the time for testing was shorter (141 vs 181 days on average), the number of test repetitions was lower (in particular for HLA-A locus, p = 0.026), and the cost reduced (240.7 vs 395.6 euros per unit on average) compared to 2013, demonstrating that Luminex xMAP technology is superior to the previous approach. Conclusion: Luminex xMAP platform has useful application in cord blood banking programs, to achieve high-definition HLA typing of cord blood units at the time of banking in a quick, accurate, and cost-effective manner.

Identification of a novel HLA-DRB1*04:94N allele by polymerase chain reaction sequence-based typing

2011 ◽  
Vol 78 (3) ◽  
pp. 226-227 ◽  
Author(s):  
F.-M. Zhu ◽  
W. Wang ◽  
W. Zhang ◽  
H.-J. Lv ◽  
L.-X. Yan
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals APO E Genotype and Familial Hypercholesterolaemia

1997 ◽  
Vol 34 (5) ◽  
pp. 534-536 ◽  
Author(s):  
E B Duly ◽  
A J Ward ◽  
C W Kirk ◽  
N C Nevin ◽  
T R Trinick ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Donor ◽  
Familial Hypercholesterolaemia ◽  
Chain Reaction ◽  
Cholesterol Levels ◽  
Apo E ◽  
Apolipoprotein E Genotypes ◽  
Ε4 Allele ◽  
Polymerase Chain ◽  
Apo E Genotype

Apolipoprotein E genotypes were measured in 83 patients with familial hypercholesterolaemia (FH) and in 175 blood donor controls. Following DNA extraction from peripheral blood, each sample was genotyped for the Apo E polymorphism by polymerase chain reaction. No significant differences were found in the levels of the ε2 and ε3 alleles between the two groups, while the ε4 allele was approximately twice as prevalent in the FH patients as in controls ( P = 0·006, df = 1). Of the FH patients, 8·4% were homozygous for the ε4 allele while this genotype was rare in controls ( P = 0·009, df = 1). These results suggest that the ε4 allele is over represented in the FH population and may contribute to increased cholesterol levels and consequent vascular disease.

Distribution ofMICAdiversity in the Chinese Han population by polymerase chain reaction sequence-based typing for exons 2-6

2009 ◽  
Vol 73 (4) ◽  
pp. 358-363 ◽  
Author(s):  
F. Zhu ◽  
H. Zhao ◽  
Y. He ◽  
W. Zhang ◽  
J. He ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chinese Han Population ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Chinese Han ◽  
Han Population ◽  
Polymerase Chain

A novel HLA allele,HLA-C*01:02:18, was identified by polymerase chain reaction sequence-based typing in a Chinese leukemia patient

2013 ◽  
Vol 82 (1) ◽  
pp. 65-66 ◽  
Author(s):  
S. -D. Tao ◽  
W. Zhang ◽  
F. -M. Zhu ◽  
J. He ◽  
H.-J. Lv
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Leukemia Patient ◽  
Polymerase Chain
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