Identification of a novel HLA-DRB1*04:94N allele by polymerase chain reaction sequence-based typing

2011 ◽  
Vol 78 (3) ◽  
pp. 226-227 ◽  
Author(s):  
F.-M. Zhu ◽  
W. Wang ◽  
W. Zhang ◽  
H.-J. Lv ◽  
L.-X. Yan
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Extração de DNA a partir de sangue humano coagulado para aplicação nas técnicas de genotipagem de antígenos leucocitários humanos e de receptores semelhantes à imunoglobulina

2009 ◽  
Vol 42 (6) ◽  
pp. 651-656 ◽  
Author(s):  
Daniela Maira Cardozo ◽  
Gláucia Andréia Guelsin ◽  
Samaia Laface Clementino ◽  
Fabiano Cavalcante de Melo ◽  
Marco Antônio Braga ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Killer Cell ◽  
Human Leukocyte ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Specific Sequence ◽  
Salting Out ◽  
Leukocyte Antigens ◽  
Polymerase Chain ◽  
Made In

O objetivo deste estudo foi padronizar uma metodologia de extração de DNA de alta qualidade a partir de amostras de sangue coagulado. Quarenta e oito amostras de sangue humano coagulado foram utilizadas para a extração de DNA pelo kit comercial EZ-DNA® (Biological Industries, Beit Haemek, Israel), pelo kit de coluna Neoscience® (One Lambda Inc., San Diego, CA) e pelo método modificado de salting out. Apenas o método de salting out foi capaz de extrair altas concentrações de DNA (média, 180ng/µL), as quais foram medidas pelo detector de fluorescência Qubit® (Invitrogen, USA). Este método permitiu a amplificação dos genes HLA (human leukocyte antigens) pela tecnologia PCR-SSO (polymerase chain reaction - specific sequence of oligonucleotides) Luminex, a qual exige DNA de boa qualidade, e de genes KIR (killer cell immunoglobulin-like receptors) pela técnica made in house PCR-SSP (polymerase chain reaction-sequence specific of primers), a qual demanda uma concentração específica de DNA (10ng/µL). Concluímos que a técnica de salting out modificada foi muito eficiente, simples e rápida para a extração de DNA de amostras de sangue humano coagulado, com o objetivo de realizar a genotipagem de genes HLA e KIR.

Identification of the novel KIR3DL2*114 allele in a Chinese individual by polymerase chain reaction sequence‐based typing

HLA ◽  
2020 ◽  
Vol 95 (6) ◽  
pp. 596-598
Author(s):  
Chen Chen ◽  
Jielin Wang ◽  
Yanmin He ◽  
Ji He ◽  
Faming Zhu
Keyword(s):  
Polymerase Chain Reaction ◽  
The Novel ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Chinese Individual

Variability among members of the Hor-2 multigene family

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 397-403 ◽  
Author(s):  
Vladimir Kanazin ◽  
Evgeny Ananiev ◽  
Tom Blake
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Family ◽  
Storage Proteins ◽  
Gene Families ◽  
Reaction Sequence ◽  
Chain Reaction ◽  
Genes Encoding ◽  
Polymerase Chain ◽  
Encoding Gene ◽  
Barley Genotypes

The hordeins comprise the major prolamin storage proteins of barley. Two major and one minor gene families encode these alcohol-soluble proteins. The Hor-2 gene family encoding the B-hordeins has been estimated to contain 15–30 copies. Although several genes encoding B-hordeins have been cloned and sequenced, little is known about the mechanisms responsible for the generation of the enormous genetic variability at this locus. Polymerase chain reaction sequence amplification provided a simple technique that permitted the amplification of the Hor-2 gene family members from the genomes of several barley genotypes. Sequence analysis of clones permitted the identification of a region within the Hor-2 structural gene that appears to undergo recombinational and slippage-like gene conversion events. In this report we describe variability of the B-hordein genes, possible mechanisms responsible for it, and implications this may have on the evolution of prolamin-encoding gene families.Key words: barley, hordeins, polymerase chain reaction, polymorphism.

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