scholarly journals Hypothermic oxygenated Machine Perfusion of the Human Pancreas for Clinical Islet Isolation: A Prospective Feasibility Study

2021 ◽  
Author(s):  
Jason B. Doppenberg ◽  
Marjolein Leemkuil ◽  
Marten A. Engelse ◽  
Christina Krikke ◽  
Eelco J.P. de Koning ◽  
...  
Keyword(s):  
Feasibility Study ◽  
Human Pancreas ◽  
Islet Isolation ◽  
Machine Perfusion

Re-evaluation of donor factors affecting islet isolation from human pancreas using a two-step digestion method

1997 ◽  
Vol 29 (4) ◽  
pp. 1969 ◽  
Author(s):  
S. Une ◽  
A. Atiya ◽  
S. Ohtsuka ◽  
S. Arita ◽  
T. Kawahara ◽  
...  
Keyword(s):  
Human Pancreas ◽  
Islet Isolation ◽  
Digestion Method ◽  
Factors Affecting ◽  
Donor Factors

scholarly journals Twenty-Four Hour Hypothermic Machine Perfusion Preservation of Porcine Pancreas Facilitates Processing for Islet Isolation

2008 ◽  
Vol 40 (2) ◽  
pp. 480-482 ◽  
Author(s):  
M.J. Taylor ◽  
S. Baicu ◽  
B. Leman ◽  
E. Greene ◽  
A. Vazquez ◽  
...  
Keyword(s):  
Islet Isolation ◽  
Machine Perfusion ◽  
Porcine Pancreas ◽  
Hypothermic Machine Perfusion

Amount and Distribution of Collagen in Pancreatic Tissue of Different Species in the Perspective of Islet Isolation Procedures

1995 ◽  
Vol 4 (6) ◽  
pp. 609-614 ◽  
Author(s):  
Paul T.R. van Suylichem ◽  
Jan-Erik H.M. van Deijnen ◽  
Gerrit H.J. Wolters ◽  
Reinout van Schilfgaarde
Keyword(s):  
Colorimetric Method ◽  
Pancreatic Tissue ◽  
Collagen Content ◽  
Human Pancreas ◽  
Islet Isolation ◽  
Secondary Importance ◽  
Enzymatic Dissociation ◽  
Tissue Sections ◽  
Dog Pancreas ◽  
General Experience

Because collagen is the major target in the enzymatic dissociation of the pancreas for islet isolation, we determined the amount of collagen and its distribution in a comparative study comprising normal pancreata of rat, dog, man, young pig, and adult pig. Collagen content was determined using a colorimetric method and its distribution was assessed in tissue sections stained with Sirius red. The collagen content is relatively low in the rat and adult pig pancreas, and the amount of collagen is relatively low in the septa of the rat and dog pancreas. Not the amount of collagen in the septa but collagen in the rest of the pancreas, mainly located between the acini, seems to determine the dissociation of the pancreatic tissue. This can be exemplified by the higher islet yields obtained from the adult vs. the young pig pancreas; the latter contains a higher total amount of collagen but a similar, relatively high, amount of collagen in the septa. A high amount of collagen surrounding the islets seems to be of secondary importance in islet isolations, because yields of the same magnitude are obtained from the canine and human pancreas containing a relatively low vs. high amount of collagen around the islets but a similar total collagen content. The rat pancreas contains both a low total amount of collagen and a high amount of collagen around the islets; therefore, the general experience that islet isolation procedures are effective in rats can be readily understood.

Hypothermic machine perfusion of the pancreas; a promising preservation method for islet isolation

2014 ◽  
Vol 31 (4) ◽  
pp. 248-249
Author(s):  
M. Leemkuil ◽  
H.G.D. Leuvenink ◽  
M.A. Engelse ◽  
R.J. Ploeg ◽  
E.J.P. de Koning ◽  
...  
Keyword(s):  
Islet Isolation ◽  
Machine Perfusion ◽  
Preservation Method ◽  
Hypothermic Machine Perfusion

PROLONGED PRESERVATION OF HUMAN PANCREAS BEFORE ISLET ISOLATION WITH THE TWO-LAYER COLD STORAGE METHOD

1999 ◽  
Vol 67 (9) ◽  
pp. S645 ◽  
Author(s):  
Y. Tanioka ◽  
Y. Suzuki ◽  
T. Deai ◽  
K. Suzuki ◽  
I. Matsumoto ◽  
...  
Keyword(s):  
Cold Storage ◽  
Human Pancreas ◽  
Islet Isolation ◽  
Storage Method

The Effect of UW Solution and Its Components on the Collagenase Digestion of Human and Porcine Pancreas

1995 ◽  
Vol 4 (6) ◽  
pp. 615-619 ◽  
Author(s):  
Harold H. Contractor ◽  
Paul R. V. Johnson ◽  
David R. Chadwick ◽  
Gavin S. M. Robertson ◽  
Nicholas J. M. London
Keyword(s):  
Cold Storage ◽  
Species Difference ◽  
Human Pancreas ◽  
Islet Isolation ◽  
Porcine Pancreas ◽  
Uw Solution ◽  
Porcine Islet ◽  
University Of Wisconsin ◽  
Collagenase Digestion ◽  
Tissue Dissociation

University of Wisconsin (UW) solution is used extensively as a cold storage solution during the procurement and transport of the pancreas prior to islet isolation. However, it has been observed that UW inhibits the collagenase digestion phase of human but not porcine islet isolation, resulting in poor islet yields and islets of poor viability. The aim of this study was, therefore, to confirm this species difference and to determine which components of UW are responsible for the inhibition in the human. In the initial experiment, blocks of human and porcine pancreas (n = 7) were incubated in test tubes containing collagenase at a concentration of 4 mg/mL at 37°C dissolved in 4 mL of either Hanks' solution or UW. Every 5 min the tubes were manually shaken and the degree of tissue dissociation scored on a scale of + and +++. Our results confirm the inhibition of collagenase digestion in the human but not the pig. Using the same methodology, we then investigated the components of UW that were causing the observed inhibition in the human pancreas (n = 7). This time the collagenase was dissolved in individual or combinations of UW components. Using Hank's as a control, the results were then expressed as a median ratio. The components found to be most inhibitory were magnesium, the Na+/K+ ratio, hydroxyethyl starch (HES), and adenosine. Allopurinol in combination with either lactobionate or glutathione was markedly inhibitory (i.e., median ratio 1.8 and 1.9, respectively). The most inhibitory solution tested was a combination of the three components raffinose, glutathione, and lactobionate (median ratio 2.1). This combination was almost as inhibitory as UW itself (median ratio 2.7). These findings are essential for the development of effective cold-storage solutions for the human pancreas that do not inhibit the subsequent collagenase digestion phase of islet isolation.

scholarly journals The Choice of Enzyme for Human Pancreas Digestion Is a Critical Factor for Increasing the Success of Islet Isolation

2015 ◽  
Vol 1 (4) ◽  
pp. 1-9 ◽  
Author(s):  
Meirigeng Qi ◽  
Luis Valiente ◽  
Brian McFadden ◽  
Keiko Omori ◽  
Shiela Bilbao ◽  
...  
Keyword(s):  
Critical Factor ◽  
Human Pancreas ◽  
Islet Isolation

Hypothermic Liver Machine Perfusion With EKPS-1 Solution vs Aqix RS-I Solution: In Vivo Feasibility Study in a Pig Transplantation Model

2009 ◽  
Vol 41 (2) ◽  
pp. 617-621 ◽  
Author(s):  
K. Vekemans ◽  
Q. Liu ◽  
V. Heedfeld ◽  
K. Van de Vel ◽  
T. Wylin ◽  
...  
Keyword(s):  
Feasibility Study ◽  
Machine Perfusion ◽  
Liver Machine Perfusion ◽  
Transplantation Model

scholarly journals Effective Islet Isolation Method with Extremely High Islet Yields from Adult Pigs

2005 ◽  
Vol 14 (10) ◽  
pp. 757-762 ◽  
Author(s):  
Yukihide Yonekawa ◽  
Shinichi Matsumoto ◽  
Teru Okitsu ◽  
Takashi Arata ◽  
Yasuhiro Iwanaga ◽  
...  
Keyword(s):  
Large Scale ◽  
New Method ◽  
Human Pancreas ◽  
Islet Isolation ◽  
Isolation Method ◽  
Porcine Pancreas ◽  
Porcine Islet ◽  
Single Donor

Achieving good islet isolation is one of the most important factors for successful islet transplantation. Porcine pancreas is suitable for islet isolation research due to its anatomical and physiological similarities to human pancreas. In this study, we evaluated a new porcine islet isolation method designed to maximize islet yield and compared it with our previous open pan method and the standard method using a Ricordi chamber (Ricordi method). We performed 15 porcine islet isolations, five each with the new method, the open pan method, and the Ricordi method. The new method features several important improvements. Pancreata remain uncut and are kept intact during collagenase intraductal injection, a large filtration chamber to handle whole pancreata, low concentration of collagenase (Liberase™ HI) for digestion, and large plastic containers for large-scale islet purification. All isolated islets were assessed for yield, purity, viability and in vitro function. Islets isolated with this new method were transplanted under the kidney capsules of SCID mice with chemically induced diabetes for in vivo functional assessment (n = 8). With the new method, we obtained on average more than 1,000,000 islet equivalents (IE) (1,236,266 ± 213,486 IE) (mean ± SE) before purification and 800,000 IE (879,815 ± 222,729 IE) after purification from one adult pig. Islet yield per pancreas was significantly higher compared with our previous open pan method (30,666 ± 11,532 IE, p < 0.01) and the Ricordi method (317,073 ± 86,093 IE, p < 0.05). All mice, transplanted with 1000 islets from the new method, returned to normoglycemia within 4 days after transplantation. Our new method makes it possible to obtain extremely high porcine islet yield with good function. It should produce useful information for human islet isolation and transplantation, and might be applied to single donor clinical xenogeneic transplantation.

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