Deep Q-Learning-Based Neural Network with Privacy Preservation Method for Secure Data Transmission in Internet of Things (IoT) Healthcare Application

The healthcare industry is being transformed by the Internet of Things (IoT), as it provides wide connectivity among physicians, medical devices, clinical and nursing staff, and patients to simplify the task of real-time monitoring. As the network is vast and heterogeneous, opportunities and challenges are presented in gathering and sharing information. Focusing on patient information such as health status, medical devices used by such patients must be protected to ensure safety and privacy. Healthcare information is confidentially shared among experts for analyzing healthcare and to provide treatment on time for patients. Cryptographic and biometric systems are widely used, including deep-learning (DL) techniques to authenticate and detect anomalies, andprovide security for medical systems. As sensors in the network are energy-restricted devices, security and efficiency must be balanced, which is the most important concept to be considered while deploying a security system based on deep-learning approaches. Hence, in this work, an innovative framework, the deep Q-learning-based neural network with privacy preservation method (DQ-NNPP), was designed to protect data transmission from external threats with less encryption and decryption time. This method is used to process patient data, which reduces network traffic. This process also reduces the cost and error of communication. Comparatively, the proposed model outperformed some standard approaches, such as thesecure and anonymous biometric based user authentication scheme (SAB-UAS), MSCryptoNet, and privacy-preserving disease prediction (PPDP). Specifically, the proposed method achieved accuracy of 93.74%, sensitivity of 92%, specificity of 92.1%, communication overhead of 67.08%, 58.72 ms encryption time, and 62.72 ms decryption time.

Preservation Method of Rumen Fluid Collected From Harvested Cattle Alters in vitro Digestibility of Reference Feedstuffs

The objective of our experiment was to evaluate preservation and revitalization strategies for rumen inoculum anticipating research and veterinary applications. Rumen fluid samples were collected from 12 harvested cattle. Liquid samples were divided into five 500-mL aliquots which were randomly allocated to one of five treatments in a 2 × 2 + 1 augmented factorial design. Factors included preservation method [freezing (FZN) or lyophilization (LYO)] and preservative (glycerol; + or –). A fresh control (CON) was maintained from each sample. Feedstuffs used in this experiment were alfalfa hay, Coastal bermudagrass hay, cracked maize, rice bran, and soybean meal. Reference feedstuffs were subjected to batch culture in vitro true digestibility (IVTD) and in vitro NDF digestibility (IVNDFD) assays using inoculum from each of the five treatments. There was an effect (P < 0.05) of preservation method, preservative, and their interaction for both IVTD and IVNDFD of each of the five references feedstuffs. Freezing or lyophilization of rumen inoculum reduced (P < 0.05) IVTD and IVNDFD of reference feeds relative to the CON. Despite lower degradation of feeds when frozen or lyophilized rumen fluid was used rather than fresh, differences between them in IVTD and IVNDFD suggest that, in the absence of fresh inoculum, preserved rumen fluid may be a viable option for veterinary applications, such as transfaunation, but likely will not be viable for research applications.

Viability, Stability and Biocontrol Activity in Planta of Specific Ralstonia Solanacearum Bacteriophages After Their Conservation Prior to Commercialization and Use

Ralstonia solanacearum is a pathogen that causes bacterial wilt producing severe damage in staple solanaceous crops. Traditional control has low efficacy and/or environmental impact. Recently, the bases of a new biotechnological method by lytic bacteriophages vRsoP-WF2, vRsoP-WM2 and vRsoP-WR2 with specific activity against R. solanacearum were established. However, some aspects remain unknown, such as the survival and maintenance of the lytic activity after submission to a preservation method as the lyophilization. To this end, viability and stability of lyophilized vRsoP-WF2, vRsoP-WM2 and vRsoP-WR2 and their capacity for bacterial wilt biocontrol have been determined against one pathogenic Spanish reference strain of R. solanacearum in susceptible tomato plants in different conditions and making use of various cryoprotectants. The assays carried out have shown satisfactory results with respect to the viability and stability of the bacteriophages after the lyophilization process, maintaining high titres throughout the experimental period, also with respect to the capacity of the bacteriophages for the biological control of bacterial wilt, controlling this disease in more than 50% of the plants. The results offer good prospects for the use of lyophilization as a conservation method for the lytic bacteriophages of R. solanacearum in view of their commercialization as biocontrol agents.

IMT-1 A translational research for practical use of dendritic cell-based immunotherapy against malignant glioma

Background: Although a therapeutic effect of dendritic cell (DC)-based immunotherapy, a kind of regenerative medicine, has been recognized in various types of cancer including malignant glioma, it is still impractical because of several unsolved problems. This study is aimed to solve the problems in regenerative medicine through a clinical trial of immunotherapy using fusions of DCs and glioma cells (GCs) against malignant glioma, and to put it into practical use. Methods: Primary cultured GCs and glioma stem cells (GSCs) were generated from surgical specimens of patient. DCs were generated from PBMC of same patient, and were fused with GCs and GSCs. The entire process of cell production must be performed by pairs of two cell-culture operators in a dedicated cell processing facility. We developed a remote cell-observation system for reducing hands work of operators. As a project to establish a preservation method, cryopreservation of glioma tissues, GCs/GSCs, DCs and fusion cells followed by their viability examination. Results: The remote cell-observation system worked stable in morphological observation and cell-counting for adhesion cells. A growth curve was also automatically and accurately created. Although a morphological observation of floating cells such as GSCs and DCs was possible, there was some error in counting of those cells. A project to establish a preservation method is currently underway, including the development of storage containers and storage liquids. Conclusions: Although the remote cell-observation system required some modifications at the observation site, depth of focus, etc. for floating cells, there was no problem in accuracy for adhesion cells compared with operator’s observation. This system, which can be easily installed at low cost, seemed to be helpful for practical use of regenerative medical products including this therapy. We are working on a project to establish a stable transportation and preservation method for prevalence of this treatment.

Anatomical Comparison of Two Grammatophyllum spp. (Orchidaceae) Species and Their Specific Ecological Adaptation

The genus Grammatophyllum (Orchidaceae) has two sections with a very different habitus. The Grammatophyllum from section “Grammatophyllum” has a long cylindrical pseudobulb with linear leaves, while the Grammatophyllum section “Gabertia” has a shorter pseudobulb with a larger diameter and broader lanceolate shaped leaves. The study is aimed to compare the anatomical characters of leaf and root between G. speciosum (representation of “Pattonia” section) and G. scriptum (representation of “Gabertia” section). Leaf and root sections were obtained using the mini-microtome with liquid preservation method in 10 replications. Data were analyzed statistically using a t-test at 5% significance. The results showed significant differences between the two species in the leaf’s primary vascular bundle area, velamen area, number of velamen layers, root’s cortex area, and stele area. Future research with more organs and parameters being explored and experimental research regarding its anatomical response to drought is suggested.

The Prevalence and Epidemiology of Salmonella in Retail Raw Poultry Meat in China: A Systematic Review and Meta-Analysis

Foodborne disease caused by Salmonella is an important public health concern worldwide. Animal-based food, especially poultry meat, is the main source of human salmonellosis. The objective of this study was to evaluate the prevalence and epidemiology of Salmonella contamination in raw poultry meat commercialized in China. Following the principle of systematic review, 98 sets of prevalence data were extracted from 74 publications conducted in 21 Chinese provincial regions. The random-effect model was constructed for subgrouping analysis by meat category, preservation type, and geographical location. The prevalence levels differed from high to low among raw poultry meat, including chicken, 26.4% (95% CI: 22.4–30.8%); pigeon, 22.6% (95% CI: 18.2–27.8%); duck, 10.1% (95% CI: 5.3–18.2%); and other poultry meat, 15.4% (95% CI: 12.0–19.5%). Prevalence data on the preservation type revealed that chilled poultry meat might be more likely to experience cross-contamination than non-chilled poultry meat in China. The distribution map of Salmonella for raw poultry meat showed that a higher prevalence level was found in the Shaanxi, Henan, Sichuan, and Beijing regions. All subgroups possessed high amounts of heterogeneity (I2 > 75%). The scientific data regarding the differences in prevalence levels between meat category, preservation method, and geographical region sources might be useful to improve specific interventions to effectively control the incidence of Salmonella in poultry meat.

Evaluation of RNA later as a Field-Compatible Preservation Method for Metaproteomic Analyses of Bacterium-Animal Symbioses

Metaproteomics, the large-scale identification and quantification of proteins from microbial communities, provide direct insights into the phenotypes of microorganisms on the molecular level. To ensure the integrity of the metaproteomic data, samples need to be preserved immediately after sampling to avoid changes in protein abundance patterns.

Inventory of the Different Methods of Natural Conservation of Food Crops Used in the Markets of Daloa (Ivory Coast)

Food in West Africa is one of the main sources of cash income. It plays an important role in feeding populations (rural and urban). These vegetables are very perishable foods, and generally seasonal, causing several post-harvest losses. Lack of knowledge of the natural techniques used by our peasants to conserve their various crops hampers their application, as well as their popularization. It is with this in mind that this study was initiated. It is based first of all on a survey carried out on large markets and supermarkets in the commune of Daloa (Ivory Coast), with a view to identifying the most widely used natural food conservation methods. It appears from these surveys that the open air preservation method was the most common at 80%. This method is widely used on eggplants (42.29%), tomatoes (33.66%) and okra (24.05%) in the markets. This method could favor certain physico-chemical parameters of the food crop to the detriment of others. The results of surveys carried out in the field have made it possible to show the need to popularize the food sector by training our various producers and sellers in order to know the basic basics of conservation.

Characterization and low-cost preservation of Chromobacterium violaceum strain TRFM-24 isolated from Tripura state, India

Background Chromobacterium species, through their bioactive molecules, help in combating biotic and abiotic stresses in plants and humans. The present study was aimed to identify, characterize and preserve in natural gums the violet-pigmented bacterial isolate TRFM-24 recovered from the rhizosphere soil of rice collected from Tripura state. Results Based on morphological, biochemical and 16S rRNA gene sequencing, the isolate TFRM-24 was identified as Chromobacterium violaceum (NAIMCC-B-02276; MCC 4212). The bacterium is saprophytic, free living and Gram negative. The strain was found positive for production of IAA, cellulase, xylanase and protease, and showed tolerance to salt (2.5%) and drought (-1.2 MPa). However, it showed poor biocontrol activity against soil-borne phytopathogens and nutrient-solubilizing abilitiets. C. violaceum strain TRFM-24 did not survive on tryptic soya agar (TSA) beyond 12 days between 4 and 32 °C temperature hence a method of preservation of this bacterium was attempted using different natural gums namely Acacia nilotica (babul), Anogeissus latifolia (dhavda), Boswellia serrata (salai) and Butea monosperma (palash) under different temperature regime (6–32 °C). The bacterium survived in babul gum (gum acacia), dhavda and salai solution at room temperature beyond a year. Conclusion Based on polyphasic approach, a violet-pigmented isolate TRFM-24 was identified as Chromobacterim violaceum which possessed some attributes of plant and human importance. Further, a simple and low-cost preservation method of strain TRFM-24 at room temperature was developed using natural gums such as babul, dhavda and salai gums which may be the first report to our knowledge.