scholarly journals Membrane properties of the smooth muscle cells of the rat anococcygeus muscle.

1975 ◽  
Vol 245 (1) ◽  
pp. 49-62 ◽  
Author(s):  
K E Creed
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Membrane Properties ◽  
Anococcygeus Muscle ◽  
Rat Anococcygeus Muscle

Membrane properties of smooth muscle cells in pulmonary hypertensive rats

1982 ◽  
Vol 242 (5) ◽  
pp. H907-H915 ◽  
Author(s):  
H. Suzuki ◽  
B. M. Twarog
Keyword(s):  
Smooth Muscle ◽  
Membrane Potential ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Main Pulmonary Artery ◽  
Fold Increase ◽  
Muscle Cells ◽  
Membrane Properties ◽  
Resting Membrane Potential ◽  
Chronic Pulmonary Hypertension

The membrane properties of smooth muscle cells in rat main pulmonary artery (MPA) and small pulmonary artery (SPA) were investigated during chronic normobaric hypoxia and after monocrotaline injection. As chronic pulmonary hypertension developed, pronounced differences between MPA and SPA were observed. These findings may shed light on mechanisms of smooth muscle hypertrophy. 1) The resting membrane potential of smooth muscle in MPA became less negative than the normal (depolarized), whereas the resting membrane potential of smooth muscle in SPA became more negative (hyperpolarized). 2) In MPA, both the length and time constants diminished. 3) In MPA, the maximum membrane depolarization produced by a 10-fold increase in extracellular [K+] decreased. 4) In SPA, the depolarization observed in K+-free solution was more rapid and greater in amplitude, and the transient hyperpolarization following restoration of K+-containing solution increased. 5) In SPA, initial and sustained depolarization evoked by Na+-deficient solutions were increased. 6) Depolarization in MPA was due to increased membrane permeability, perhaps to Cl-, whereas hyperpolarization in SPA could be attributed to increased activity of an electrogenic Na+-K+ pump.

Membrane properties and innervation of smooth muscle cells in Hirschsprung's disease

1983 ◽  
Vol 244 (4) ◽  
pp. G406-G415 ◽  
Author(s):  
M. Kubota ◽  
Y. Ito ◽  
K. Ikeda
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Large Intestine ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Circular Muscle ◽  
Muscle Cells ◽  
Membrane Properties ◽  
Aganglionic Segment ◽  
Excitatory Junction Potentials

Mechanical and membrane properties of smooth muscle cells and/or neuroeffector transmission in the aganglionic segment of the large intestine (Hirschsprung's disease) were compared with findings in the ganglionic segment. Tension-recording, microelectrode, and double sucrose gap methods were used. There was no difference in resting membrane potential of the longitudinal or circular muscle cell in these two segments, which were obtained at biopsy in Japanese children. In the ganglionic preparations, generations of regular prepotentials, with or without the spike, correlated well to the rhythmic contractions. However, in the aganglionic segment, irregular spike and contraction only were observed. In the circular or longitudinal muscle of the ganglionic segments, field stimulations evoked inhibitory junction potentials, excitatory junction potentials, or both and triggered initial relaxation and then a contraction of the tissue. In the aganglionic segment, however, field stimulation evoked only excitatory junction potentials followed by contraction. These results indicate that, in cases of Hirschsprung's disease, there may be a deficiency in the nonadrenergic inhibitory pathways. This is the first evidence for a lack of spasticity in muscle from the aganglionic segment of the large intestine obtained from children with Hirschsprung's disease.

Membrane properties of smooth muscle cells in pulmonary arteries of the rat

1982 ◽  
Vol 242 (5) ◽  
pp. H900-H906 ◽  
Author(s):  
H. Suzuki ◽  
B. M. Twarog
Keyword(s):  
Smooth Muscle ◽  
Membrane Potential ◽  
Pulmonary Artery ◽  
Smooth Muscle Cells ◽  
Main Pulmonary Artery ◽  
Pulmonary Arteries ◽  
Outward Current ◽  
Muscle Cells ◽  
Membrane Properties ◽  
Similar Degree

Electrical properties of the membrane of smooth muscle cells in the rat main pulmonary artery (MPA) and a small pulmonary artery (SPA) were compared. MPA and SPA differed in several important respects, suggesting characteristic quantitative and qualitative differences in membrane properties. 1) Resting membrane potentials were similar in both (MPA 52.2 +/- 1.3 mV; SPA 51.5 +/- 1.7 mV). The cells displayed no spontaneous electrical activity. The muscle layers in both MPA and SPA showed cablelike properties; a graded local response to outward current pulses was observed, but no action potentials were evoked. 2) Tetraethylammonium chloride (TEA, 1-5 mM) depolarized, increased membrane resistance, and suppressed rectification in MPA. TEA strongly depolarized SPA and contraction ensued. 3) The maximum membrane depolarization produced by a 10-fold increase in extracellular [K+] was 48 mV in MPA and 47 mV in SPA. In K+-free solution gradual depolarization was observed in SPA, but the membrane potential in MPA was not modified. Restoration of K+-containing solution produced equivalent hyperpolarization in both tissues, indicating a similar degree of stimulation of electrogenic Na+-K+ pumping. 4) A Na+-deficient solution did not affect the membrane potential in MPA but depolarized SPA.

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

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