Resolution enhancement of confocal scanning microscopy using low-intensity imaging part of point spread function

2018 ◽  
Vol 57 (05) ◽  
pp. 1 ◽  
Author(s):  
Vannhu Le ◽  
Xiaona Wang ◽  
Cuifang Kuang ◽  
Xu Liu
Keyword(s):  
Point Spread Function ◽  
Resolution Enhancement ◽  
Scanning Microscopy ◽  
Low Intensity ◽  
Point Spread ◽  
Spread Function ◽  
Confocal Scanning ◽  
Confocal Scanning Microscopy

scholarly journals Rapid 3D image scanning microscopy with multi-spot excitation and double-helix point spread function detection

2018 ◽  
Vol 26 (18) ◽  
pp. 23585 ◽  
Author(s):  
Siwei Li ◽  
Jingjing Wu ◽  
Heng Li ◽  
Danying Lin ◽  
Bin Yu ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Double Helix ◽  
Scanning Microscopy ◽  
3D Image ◽  
Image Scanning ◽  
Point Spread ◽  
Spread Function

Point-spread-function Engineering in High-NA Optical Scanning Microscopy: Desensitizing to Sample-induced Aberrations

2007 ◽  
Author(s):  
Genaro Saavedra ◽  
Isabel Escobar ◽  
Manuel Martinez-Corral ◽  
Jón Atli Benediktsson ◽  
Bahram Javidi ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Scanning Microscopy ◽  
Optical Scanning ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering

scholarly journals Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy

2019 ◽  
Author(s):  
Graeme E. Johnstone ◽  
Gemma S. Cairns ◽  
Brian R. Patton
Keyword(s):  
Adaptive Optics ◽  
Point Spread Function ◽  
Size Range ◽  
Resolution Enhancement ◽  
Super Resolution ◽  
Two Photon ◽  
Point Spread ◽  
Photon Excitation ◽  
Spread Function ◽  
Photo Stability

Particles of diamond in the 5 to 100 nm size range, known as nanodiamond, have shown promise as robust fluorophores for optical imaging. We demonstrate here that, due to their photo-stability, they are not only suitable for two-photon imaging, but they allow significant resolution enhancement when combined with computational super-resolution techniques. We observe a resolution of 42.5 nm when processing two-photon images with the Super-Resolution Radial Fluctuations algorithm. We show manipulation of the point-spread function of the microscope using adaptive optics. This demonstrates how the photostability of nanodiamond can also be of use when characterising adaptive optics technologies or testing the resilience of super-resolution or aberration correction algorithms.

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