Bacillus thuringiensis enzyme-digested delta endotoxin: effect on cultured insect cells

Science ◽  
1976 ◽  
Vol 194 (4268) ◽  
pp. 954-956 ◽  
Author(s):  
D. Murphy ◽  
S. Sohi ◽  
P. Fast
Keyword(s):  
Bacillus Thuringiensis ◽  
Insect Cells ◽  
Delta Endotoxin

scholarly journals Mechanism of action of Bacillus thuringiensis insecticidal delta-endotoxin on insect cells in vitro.

1985 ◽  
Vol 49 (5) ◽  
pp. 1461-1468 ◽  
Author(s):  
Michio HIMENO ◽  
Naoto KOYAMA ◽  
Tomohiko FUNATO ◽  
Tohru KOMANO
Keyword(s):  
Bacillus Thuringiensis ◽  
Mechanism Of Action ◽  
Insect Cells ◽  
Delta Endotoxin

scholarly journals The Bacillus thuringiensis delta-endotoxin. Evidence for a two domain structure of the minimal toxic fragment.

1990 ◽  
Vol 265 (3) ◽  
pp. 1369-1375
Author(s):  
D Convents ◽  
C Houssier ◽  
I Lasters ◽  
M Lauwereys
Keyword(s):  
Bacillus Thuringiensis ◽  
Domain Structure ◽  
Delta Endotoxin

scholarly journals Analysis of the molecular basis of insecticidal specificity of Bacillus thuringiensis crystal δ-endotoxin

1987 ◽  
Vol 248 (1) ◽  
pp. 197-201 ◽  
Author(s):  
M Z Haider ◽  
D J Ellar
Keyword(s):  
Bacillus Thuringiensis ◽  
Membrane Proteins ◽  
Insect Cell ◽  
Receptor Interaction ◽  
Dual Specificity ◽  
Delta Endotoxin ◽  
Cell Membrane Proteins ◽  
Toxin Receptor ◽  
Osmotic Lysis ◽  
Initial Interaction

The mechanism of action and receptor binding of a dual-specificity Bacillus thuringiensis var. aizawai ICl delta-endotoxin was studied using insect cell culture. The native protoxin was labelled with 125I, proteolytically activated and the affinity of the resulting preparations for insect cell-membrane proteins was studied by blotting. The active preparations obtained by various treatments had characteristic specificity associated with unique polypeptides, and showed affinity for different membrane proteins. The lepidopteran-specific preparation (trypsin-treated protoxin containing 58 and 55 kDa polypeptides) bound to two membrane proteins in the lepidopteran cells but none in the dipteran cells. The dipteran-specific preparation (protoxin treated sequentially with trypsin and Aedes aegypti gut proteases, containing a 53 kDa polypeptide) bound to a 90 kDa membrane protein in the dipteran (A. aegypti) cells but bound to none in the lepidopteran cells or Drosophila melanogaster cells. The toxicity of trypsin-activated delta-endotoxin was completely inhibited by preincubation with D-glucose, suggesting a role for this carbohydrate in toxin-receptor interaction. The toxicity was also decreased by osmotic protectants to an extent proportional to their viscometric radius. These results support a proposal that initial interaction of toxin with a unique receptor determines the specificity of the toxin, following which cell death occurs by a mechanism of colloid osmotic lysis.

scholarly journals Effects of Bacillus thuringiensis δ-Endotoxin on Insect and Mammalian Cells In Vitro

1980 ◽  
Vol 15 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Junko NISHITSUTSUJI-UWO ◽  
Yasuhisa ENDO ◽  
Michio HIMENO
Keyword(s):  
Bacillus Thuringiensis ◽  
Mammalian Cells ◽  
Delta Endotoxin

scholarly journals Plasmids and insecticidal activity of delta-endotoxin crystals from Bacillus thuringiensis var. israelensis.

1985 ◽  
Vol 49 (3) ◽  
pp. 573-580 ◽  
Author(s):  
Michio HIMENO ◽  
Masao IKEDA ◽  
Kikuo SEN ◽  
Naoto KOYAMA ◽  
Tohru KOMANO ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Delta Endotoxin

Expression of Bacillus thuringiensis Toxins in Insect Cells

2017 ◽  
pp. 99-110 ◽  
Author(s):  
Bergmann Morais Ribeiro ◽  
Érica Soares Martins ◽  
Raimundo Wagner de Souza Aguiar ◽  
Roberto Franco Teixeira Corrêa
Keyword(s):  
Bacillus Thuringiensis ◽  
Insect Cells

Improvement of delta-endotoxin production from local Bacillus thuringiensis Se13 using Taguchi’s orthogonal array methodology

2018 ◽  
Vol 43 (6) ◽  
pp. 662-670 ◽  
Author(s):  
Ardahan Eski ◽  
Zihni Demirbağ ◽  
İsmail Demir
Keyword(s):  
Bacillus Thuringiensis ◽  
Incubation Period ◽  
Orthogonal Array ◽  
Incubation Temperature ◽  
Taguchi Methods ◽  
Optimum Level ◽  
Optimum Conditions ◽  
Delta Endotoxin ◽  
Initial Ph ◽  
Four Levels

Abstract Objective The insecticidal activity of Bacillus thuringiensis directly depends on the yield of delta-endotoxins. In this study, various nutritional and cultural parameters influencing delta-endotoxin synthesis by a local isolate of B. thuringiensis Se13 were investigated using Taguchi methods. Methods In the first experiment, four factors, incubation period, incubation temperature, initial pH and medium, each at four levels, were selected and an orthogonal array layout of L16 was carried out. In the second experiment, Taguchi’s orthogonal array method of L27 was used to evaluate the effects of the different concentration of medium components. Taguchi’s signal–noise ratio and variance analysis were applied to determine the effect of the factors. After each experiment, verification studies were carried out using determined optimum conditions. Results The optimum conditions for incubation period, incubation temperature, initial pH, and medium determined as 72 h, 30°C, pH 9, and M4 medium, respectively. In the second experiment, soybean flour (5%), glucose (5%), KH2PO4 (0.3%), K2HPO4 (0.1%), MgSO4 (0.4%) were determined as the optimum conditions. The delta-endotoxin yield was elevated to 1559.25 μg mL−1 when the factors were adjusted to optimum level. Conclusion Optimization using the Taguchi method appeared to be a good choice for the overproduction of delta-endotoxin.

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