A P(V) platform for oligonucleotide synthesis

Science ◽  
2021 ◽  
Vol 373 (6560) ◽  
pp. 1265-1270 ◽  
Author(s):  
Yazhong Huang ◽  
Kyle W. Knouse ◽  
Shenjie Qiu ◽  
Wei Hao ◽  
Natalia M. Padial ◽  
...  
Keyword(s):  
Oligonucleotide Synthesis

New coloured protecting groups in oligonucleotide synthesis cleavable by β-elimination

1993 ◽  
Vol 58 (s1) ◽  
pp. 163-166
Author(s):  
Markus Beier ◽  
Wolfgang Pfleiderer
Keyword(s):  
Protecting Groups ◽  
Oligonucleotide Synthesis

Base-labile 5'-OH protecting groups - A new approach in oligonucleotide synthesis

1990 ◽  
Vol 55 (s1) ◽  
pp. 197-200
Author(s):  
Helga Schirmeister ◽  
Wolfgang Pfleiderer
Keyword(s):  
Protecting Groups ◽  
Oligonucleotide Synthesis ◽  
New Approach

Oligonucleotide synthesis interfaced with molecular biology and nanotechnology

2014 ◽  
Author(s):  
Marvin H. Caruthers ◽  
Subhadeep Roy ◽  
Heera Krishna ◽  
Ayman Alawneh ◽  
Shibasich Paul ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Oligonucleotide Synthesis

Sulfur-containing phosphonate monomers for oligonucleotide synthesis

2014 ◽  
Author(s):  
Ondřej Kostov ◽  
Eva Zborníková ◽  
Miloš Buděšínský ◽  
Pavel Novák ◽  
Ivan Rosenberg
Keyword(s):  
Oligonucleotide Synthesis ◽  
Sulfur Containing

Fully Automated Parallel Oligonucleotide Synthesizer

2001 ◽  
Vol 66 (8) ◽  
pp. 1299-1314 ◽  
Author(s):  
Michal Lebl ◽  
Christine Burger ◽  
Brett Ellman ◽  
David Heiner ◽  
Georges Ibrahim ◽  
...  
Keyword(s):  
Solid Phase ◽  
Building Blocks ◽  
Solid Support ◽  
Continuous Operation ◽  
Gear Pump ◽  
Oligonucleotide Synthesis ◽  
Center Of Rotation ◽  
Microtiter Plates ◽  
The Individual ◽  
Design And Construction

Design and construction of automated synthesizers using the tilted plate centrifugation technology is described. Wash solutions and reagents common to all synthesized species are delivered automatically through a 96-channel distributor connected to a gear pump through two four-port selector valves. Building blocks and other specific reagents are delivered automatically through banks of solenoid valves, positioned over the individual wells of the microtiterplate. These instruments have the following capabilities: Parallel solid-phase oligonucleotide synthesis in the wells of polypropylene microtiter plates, which are slightly tilted down towards the center of rotation, thus generating a pocket in each well, in which the solid support is collected during centrifugation, while the liquid is expelled from the wells. Eight microtiterplates are processed simultaneously, providing thus a synthesizer with a capacity of 768 parallel syntheses. The instruments are capable of unattended continuous operation, providing thus a capacity of over two millions 20-mer oligonucleotides in a year.

Improved Conditions for Solid Phase Synthesis of Oligonucleotides on PS-PEG Copolymers

1995 ◽  
Vol 50 (7) ◽  
pp. 1096-1100 ◽  
Author(s):  
Ernst Bayer ◽  
Konrad Bleicher ◽  
Martin Maier
Keyword(s):  
Electrospray Mass Spectrometry ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Chemical Properties ◽  
Oligonucleotide Synthesis ◽  
Phase Synthesis ◽  
Controlled Pore Glass ◽  
Product Identification ◽  
Pore Glass ◽  
And Chemical Properties

Polystyrene-polyethylene glycol (PS-PEG) tentacle polymers with loadings of up to 60/<μmol/g were used for standard oligonucleotide synthesis. As these resins are easy to handle and stable under reaction and cleavage conditions they may be used alternatively to controlled pore glass (CPG) as the most commonly used solid support for oligonucleotide synthesis. However, structural and chemical properties of the PS-PEG resins require modified conditions to guarantee syntheses with high coupling efficiencies. Oligonucleotides (ODN ) of various sequences and lengths have successfully been synthesized using HPLC and capillary electrophoresis (CE) for purity control. Additionally, electrospray mass spectrometry (ES-MS) was used for product identification.

scholarly journals Protection of Nucleosides for Oligonucleotide Synthesis

2005 ◽  
Vol 23 (1) ◽  
Author(s):  
Serge L. Beaucage
Keyword(s):  
Oligonucleotide Synthesis

ChemInform Abstract: Nucleotide Chemistry. Part 16. Amidine Protecting Groups for Oligonucleotide Synthesis.

1986 ◽  
Vol 17 (32) ◽  
Author(s):  
L. J. MCBRIDE ◽  
R. KIERZEK ◽  
S. L. BEAUCAGE ◽  
M. H. CARUTHERS
Keyword(s):  
Protecting Groups ◽  
Oligonucleotide Synthesis

ChemInform Abstract: INTERNUCLEOTIDE CLEAVAGE DURING UNBLOCKING IN OLIGONUCLEOTIDE SYNTHESIS BY THE PHOSPHOTRIESTER APPROACH

1975 ◽  
Vol 6 (4) ◽  
pp. no-no
Author(s):  
J. H. VAN BOOM ◽  
P. M. J. BURGERS ◽  
P. H. VAN DEURSEN
Keyword(s):  
Oligonucleotide Synthesis
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