Microbial Strain Typing for Epidemiology and Infection Control

2016 ◽  
pp. 13.5.1-13.5.7
2001 ◽  
Vol 47 (7) ◽  
pp. 667-669 ◽  
Author(s):  
Sophie Michaud ◽  
Robert D Arbeit ◽  
Christiane Gaudreau

Rapid molecular strain typing is critical for effective outbreak investigation and implementation of infection control measures. Pulsed-field gel electrophoresis is a highly discriminatory technique for Campylobacter jejuni, but generally requires 3–5 days. We describe a simplified protocol for pulsed-field gel electrophoresis that provides high quality typing of C. jejuni isolates in a single day.Key words: pulsed-field gel electrophoresis, Campylobacter jejuni, molecular epidemiology.


2015 ◽  
Vol 54 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Larry K. Kociolek ◽  
Dale N. Gerding

Clostridium difficilestrain BI/NAP1/027 is associated with increasedC. difficileinfection (CDI) rates and severity, and the efficacy of some CDI therapies may be strain dependent. Although culturedC. difficileisolates can be reliably subtyped by various methods, the long turnaround times, high cost, and limited availability of strain typing preclude their routine use. Nucleic acid amplification tests identify BI/NAP1/027 rapidly from stool, but the emergence of closely related strains compromises test specificity. Although detection of epidemiologically significant pathogens is generally useful for infection control programs, specific data supporting use of rapid detection of BI/NAP1/027 as an infection control tool are still awaited.


1989 ◽  
Vol 53 (4) ◽  
pp. 222-225 ◽  
Author(s):  
JO Katz ◽  
JA Cottone ◽  
PK Hardman ◽  
TS Taylor

1988 ◽  
Vol 52 (4) ◽  
pp. 192-197
Author(s):  
SB Corbin ◽  
MI Scarlett ◽  
EW Mitchell

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