Mating-type Gene Switching in Saccharomyces cerevisiae

2015 ◽  
pp. 491-514
Author(s):  
Cheng-Sheng Lee ◽  
James E. Haber
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Mating Type Gene ◽  
Type Gene ◽  
Gene Switching

scholarly journals The sum1-1 mutation affects silent mating-type gene transcription in Saccharomyces cerevisiae.

1990 ◽  
Vol 10 (1) ◽  
pp. 409-412 ◽  
Author(s):  
G P Livi ◽  
J B Hicks ◽  
A J Klar
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Transcriptional Control ◽  
Gene Mutations ◽  
State Level ◽  
Mating Type Gene ◽  
Gene Transcripts ◽  
Mating Type Genes ◽  
Type Gene ◽  
Type Information

The silent mating-type genes (HML and HMR) of Saccharomyces cerevisiae are kept under negative transcriptional control by the trans-acting products of the four MAR/SIR loci. MAR/SIR gene mutations result in the simultaneous derepression of HML and HMR gene expression. The sum1-1 mutation was previously identified as an extragenic suppressor of mutations in MAR1 (SIR2) and MAR2 (SIR3). As assayed genetically, sum1-1 is capable of restoring repression of silent mating-type information in cells containing mar1 or mar2 null mutations. We show here that the mating-type phenotype associated with sum1-1 results from a dramatic reduction in the steady-state level of HML and HMR gene transcripts. At the same time, the sum1-1 mutation has no significant effect on the level of each of the four MAR/SIR mRNAs.

The sum1-1 mutation affects silent mating-type gene transcription in Saccharomyces cerevisiae

1990 ◽  
Vol 10 (1) ◽  
pp. 409-412
Author(s):  
G P Livi ◽  
J B Hicks ◽  
A J Klar
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Transcriptional Control ◽  
Gene Mutations ◽  
State Level ◽  
Mating Type Gene ◽  
Gene Transcripts ◽  
Mating Type Genes ◽  
Type Gene ◽  
Type Information

The silent mating-type genes (HML and HMR) of Saccharomyces cerevisiae are kept under negative transcriptional control by the trans-acting products of the four MAR/SIR loci. MAR/SIR gene mutations result in the simultaneous derepression of HML and HMR gene expression. The sum1-1 mutation was previously identified as an extragenic suppressor of mutations in MAR1 (SIR2) and MAR2 (SIR3). As assayed genetically, sum1-1 is capable of restoring repression of silent mating-type information in cells containing mar1 or mar2 null mutations. We show here that the mating-type phenotype associated with sum1-1 results from a dramatic reduction in the steady-state level of HML and HMR gene transcripts. At the same time, the sum1-1 mutation has no significant effect on the level of each of the four MAR/SIR mRNAs.

Mating-type gene switching in a homothallic fission yeast

1984 ◽  
Vol 110 (2) ◽  
pp. 299-312 ◽  
Author(s):  
Byron F. Johnson ◽  
G.B. Calleja ◽  
M. Zuker
Keyword(s):  
Fission Yeast ◽  
Mating Type ◽  
Mating Type Gene ◽  
Type Gene ◽  
Gene Switching

scholarly journals Deletion of the Schizophyllum commune Aα Locus: The Roles of Aα Y and Z Mating-Type Genes

Genetics ◽  
1996 ◽  
Vol 144 (4) ◽  
pp. 1437-1444
Author(s):  
C Ian Robertson ◽  
Kirk A Bartholomew ◽  
Charles P Novotny ◽  
Robert C Ullrich
Keyword(s):  
Mating Type ◽  
Sexual Development ◽  
Schizophyllum Commune ◽  
Mating Types ◽  
Developmental Pathway ◽  
Mating Type Gene ◽  
Mating Type Genes ◽  
Regulatory Loci ◽  
Type Gene

The Aα locus is one of four master regulatory loci that determine mating type and regulate sexual development in Schizophyllum commune. We have made a plasmid containing a URA1 gene disruption of the Aα Y1 gene. Y1 is the sole Aα gene in Aα1 strains. We used the plasmid construction to produce an Aα null (i.e., AαΔ) strain by replacing the genomic Y1 gene with URA1 in an Aα1 strain. To characterize the role of the Aα genes in the regulation of sexual development, we transformed various Aα Y and Z alleles into AαΔ strains and examined the acquired mating types and mating abilities of the transformants. These experiments demonstrate that the Aα Y gene is not essential for fungal viability and growth, that a solitary Z Aα mating-type gene does not itself activate development, that Aβ proteins are sufficient to activate the A developmental pathway in the absence of Aα proteins and confirm that Y and Z genes are the sole determinants of Aα mating type. The data from these experiments support and refine our model of the regulation of A-pathway events by Y and Z proteins.

Homothallic switching of Saccharomyces cerevisiae mating type genes by using a donor containing a large internal deletion

1985 ◽  
Vol 5 (8) ◽  
pp. 2154-2158
Author(s):  
B Weiffenbach ◽  
J E Haber
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Mating Type ◽  
Gene Conversion Event ◽  
Conversion Event ◽  
Base Pair Deletion ◽  
Mating Type Genes ◽  
Type Gene ◽  
Mat Locus ◽  
Donor Locus

Homothallic switching of the mating type genes of Saccharomyces cerevisiae occurs by a gene conversion event, replacing sequences at the expressed MAT locus with a DNA segment copied from one of two unexpressed loci, HML or HMR. The transposed Ya or Y alpha sequences are flanked by homologous regions that are believed to be essential for switching. We examined the transposition of a mating type gene (hmr alpha 1-delta 6) which contains a 150-base-pair deletion spanning the site where the HO endonuclease generates a double-stranded break in MAT that initiates the gene conversion event. Despite the fact that the ends of the cut MAT region no longer share homology with the donor hmr alpha 1-delta 6, switching of MATa or MAT alpha to mat alpha 1-delta 6 was efficient. However, there was a marked increase in the number of aberrant events, especially the formation of haploid-inviable fusions between MAT and the hmr alpha 1-delta 6 donor locus.

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