scholarly journals Kanamycin Susceptibility Testing of Mycobacterium tuberculosis Using Mycobacterium Growth Indicator Tube and a Colorimetric Method

2001 ◽  
Vol 45 (6) ◽  
pp. 1934-1936 ◽  
Author(s):  
Ivan Bastian ◽  
Leen Rigouts ◽  
Juan Carlos Palomino ◽  
Françoise Portaels
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Susceptibility Testing ◽  
Colorimetric Method ◽  
Alamar Blue ◽  
Mycobacterium Growth Indicator Tube ◽  
Indicator Tube ◽  
Proportion Method ◽  
Susceptibility Tests ◽  
Growth Indicator

ABSTRACT Two novel systems were evaluated for performing indirect kanamycin susceptibility tests on 72 strains of Mycobacterium tuberculosis. The microplate Alamar blue colorimetric method (breakpoint, 2.5 μg/ml) and the Mycobacterium Growth Indicator Tube (MGIT) system (breakpoint, 5.0 μg/ml) both produced 98.6% agreement when compared with the conventional proportion method performed on 7H10 agar using 5.0 μg of kanamycin/ml. Both systems provided results within an average of 1 week.

scholarly journals Occurrence ofrpoBMutations in Isoniazid-Resistant but Rifampin-Susceptible Mycobacterium tuberculosis Isolates from Germany

2013 ◽  
Vol 58 (1) ◽  
pp. 590-592 ◽  
Author(s):  
Sönke Andres ◽  
Doris Hillemann ◽  
Sabine Rüsch-Gerdes ◽  
Elvira Richter
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Content Type ◽  
Indicator Tube ◽  
Lowenstein Jensen ◽  
Proportion Method ◽  
Rifampin Resistance ◽  
Growth Indicator

ABSTRACTFour out of 143 phenotypically isoniazid-resistant but rifampin-susceptibleMycobacterium tuberculosisstrains that were isolated from patients in Germany in 2011 had mutations in the rifampin resistance-determining region ofrpoB. After performing drug susceptibility testing (DST) with two methods, the proportion method on Löwenstein-Jensen medium and using the Bactec 960 Mycobacteria Growth Indicator Tube system, we conclude that the two methods are equally reliable for phenotypic DST and MIC determination.

scholarly journals Multicenter Evaluation of the Mycobacteria Growth Indicator Tube for Testing Susceptibility of Mycobacterium tuberculosis to First-Line Drugs

1999 ◽  
Vol 37 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Sabine Rüsch-Gerdes ◽  
Cornelia Domehl ◽  
Giampietro Nardi ◽  
Maria Rita Gismondo ◽  
Hans-Martin Welscher ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Susceptibility Testing ◽  
Solid Medium ◽  
First Line ◽  
Indicator Tube ◽  
Significant Difference ◽  
Testing Susceptibility ◽  
Proportion Method ◽  
Test Cultures ◽  
Growth Indicator

In a multicenter study involving three reference centers for mycobacteria, the reliability of the Mycobacteria Growth Indicator Tube (MGIT) for rapid antimicrobial susceptibility testing (AST) ofMycobacterium tuberculosis was evaluated and compared to the radiometric method (BACTEC 460TB). Test cultures for which the results of the MGIT and BACTEC 460TB tests were discordant were checked by the conventional proportion method on solid medium. Four hundred forty-one isolates have been tested for susceptibility to isoniazid (INH), rifampin (RMP), ethambutol (EMB), and streptomycin (SM). Discrepant results were obtained for three isolates (0.7%) with INH (susceptible by MGIT, resistant by BACTEC 460TB), for four isolates (0.9%) with RMP (susceptible by MGIT, resistant by BACTEC 460TB), for six isolates (1.9%) with EMB (four susceptible by MGIT, resistant by BACTEC 460TB; two resistant by MGIT, susceptible by BACTEC 460TB), and for four isolates (0.9%) with SM (two susceptible by MGIT, resistant by BACTEC 460TB; two resistant by MGIT, susceptible by BACTEC 460TB). When cultures with discordant results were tested by the conventional proportion method, about half of the cultures yielded results similar to the BACTEC 460TB results, while the other half yielded results similar to the MGIT results. Turnaround times were 3 to 14 days (median, 8.8 days) for MGIT and 3 to 15 days (median, 7.8 days) for BACTEC 460TB. There was no statistically significant difference between the susceptibility testing results of the two methods (P > 0.05). These data demonstrate that the MGIT system is an accurate, nonradiometric alternative to the BACTEC 460TB method for rapid susceptibility testing of M. tuberculosis.

Comparison of the Manual Mycobacteria Growth Indicator Tube and the Etest with the Method of Proportion for Susceptibility Testing of Mycobacterium tuberculosis

Chemotherapy ◽  
2006 ◽  
Vol 52 (4) ◽  
pp. 174-177 ◽  
Author(s):  
E. Fegou ◽  
E. Jelastopulu ◽  
S. Nicolaou ◽  
M. Sevdali ◽  
S. Anagnostou ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Susceptibility Testing ◽  
Indicator Tube ◽  
Growth Indicator

scholarly journals Comparison of Line Probe Assay (LPA) and Mycobacterium Growth Indicator Tubes (MGIT) Assay for Second-line TB Drug Susceptibility Testing

2021 ◽  
Vol 13 (3) ◽  
pp. 256-60
Author(s):  
Towifah Fauziah Choerunisa ◽  
Leni Lismayanti ◽  
Tiene Rostini ◽  
Ryan Bayusantika ◽  
Ida Parwati
Keyword(s):  
Susceptibility Testing ◽  
Multidrug Resistant ◽  
Second Line ◽  
Line Probe Assay ◽  
Mycobacterium Growth Indicator Tube ◽  
Indicator Tube ◽  
Significant Difference ◽  
Mdr Tb ◽  
Probe Assay ◽  
Growth Indicator

BACKGROUND: Tuberculosis (TB) infection is one of the most prominent health issues in the world, including in Indonesia. TB is evolving into multidrug-resistant tuberculosis (MDR-TB) and requiring second-line TB drugs. Mycobacterium growth indicator tube (MGIT) is the gold standard for susceptibility testing of second-line TB drugs. Alternatively, line probe assay (LPA), which detects genes resistant to second-line TB drugs, takes a shorter time to run. This study aims to compare MGIT and LPA's ability to detect TB resistance to second-line TB drugs and observe mutation patterns of genes encoding second-line TB drugs.METHODS: This was an observational analytic study, using cross-sectional method. The data were acquired from the MDR-TB clinic’s medical records at the Dr. Hasan Sadikin Hospital from September to December 2019. LPA and MGIT test were conducted at the Health Laboratory Hall of West Java Province, then tested using Kolmogorov-Smirnov and chi-square statistic.RESULTS: From 121 subjects, 113 people were not resistant to the second-line TB drugs, which was examined using both LPA and MGIT (93.4%), p=0.991. Mutations were found in gyrA and rrs gene. There was no significant difference between the proportion of subjects resistant to the second-line of TB drugs tested using LPA and MGIT.CONCLUSION: LPA is an alternative method to MGIT because it requires a shorter time and reduces the risk of exposure that will improve MDR-TB patients management.KEYWORDS: line probe assay (LPA), multidrug-resistant TB, mycobacterium growth indicator tube (MGIT), second-line TB drugs 

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