scholarly journals Detection by Radioligand Assay of Antibodies against Borna Disease Virus in Patients with Various Psychiatric Disorders

2005 ◽  
Vol 12 (5) ◽  
pp. 671-676 ◽  
Author(s):  
Hidenori Matsunaga ◽  
Susumu Tanaka ◽  
Fuyoko Sasao ◽  
Yoshii Nishino ◽  
Masatoshi Takeda ◽  
...  
Keyword(s):  
Psychiatric Disorders ◽  
Disease Virus ◽  
Western Blotting ◽  
Psychiatric Patients ◽  
Conformational Structure ◽  
Natural Form ◽  
Borna Disease Virus ◽  
Radioligand Assay ◽  
Positive Results ◽  
Borna Disease

ABSTRACT Using a radioligand assay, which preserves the natural form of the antigen, antibodies against Borna disease virus nucleoprotein and phosphoprotein were detected in 11 and 19 sera of 171 psychiatric patients, respectively. Compared with results by Western blotting, three and nine sera were concordantly positive, respectively. The four sera showing the highest levels of antibodies by radioligand assay were all negative by Western blotting; however, dilution and inhibition tests supported the positive results. Our results suggest the importance of conformational structure to detect human anti-Borna disease virus antibodies.

scholarly journals Pathogenesis of Borna Disease Virus: Granulocyte Fractions of Psychiatric Patients Harbor Infectious Virus in the Absence of Antiviral Antibodies

1999 ◽  
Vol 73 (8) ◽  
pp. 6251-6256 ◽  
Author(s):  
Oliver Planz ◽  
Christine Rentzsch ◽  
Anil Batra ◽  
Arvind Batra ◽  
Tanja Winkler ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Psychiatric Disorders ◽  
Disease Virus ◽  
Infectious Virus ◽  
Psychiatric Patients ◽  
Borna Disease Virus ◽  
Human Virus ◽  
Close Relationship ◽  
Autopsy Brain Tissue ◽  
Borna Disease

ABSTRACT Borna disease virus (BDV) causes acute and persistent infections in various vertebrates. During recent years, BDV-specific serum antibodies, BDV antigen, and BDV-specific nucleic acid were found in humans suffering from psychiatric disorders. Furthermore, viral antigen was detected in human autopsy brain tissue by immunohistochemical staining. Whether BDV infection can be associated with psychiatric disorders is still a matter of debate; no direct evidence has ever been presented. In the present study we report on (i) the detection of BDV-specific nucleic acid in human granulocyte cell fraction from three different psychiatric patients and (ii) the isolation of infectious BDV from these cells obtained from a patient with multiple psychiatric disorders. In leukocyte preparations other than granulocytes, either no BDV RNA was detected or positive PCR results were obtained only if there was at least 20% contamination with granulocytes. Parts of the antigenome of the isolated virus were sequenced, demonstrating the close relationship to the prototype BDV strains (He/80 and strain V) as well as to other human virus sequences. Our data provide strong evidence that cells in the granulocyte fraction represent the major if not the sole cell type harboring BDV-specific nucleic acid in human blood and contain infectious virus. In contrast to most other reports of putative human isolates, where sequences are virtually identical to those of the established laboratory strains, this isolate shows divergence in the region previously defined as variable in BDV from naturally infected animals.

scholarly journals Immunological and PCR Analyses for Borna Disease Virus in Psychiatric Patients and Blood Donors in Japan

2001 ◽  
Vol 39 (2) ◽  
pp. 419-429 ◽  
Author(s):  
K. Fukuda ◽  
K. Takahashi ◽  
Y. Iwata ◽  
N. Mori ◽  
K. Gonda ◽  
...  
Keyword(s):  
Disease Virus ◽  
Blood Donors ◽  
Psychiatric Patients ◽  
Borna Disease Virus ◽  
Borna Disease

Borna Disease Virus infection in children with psychiatric disorders

Apmis ◽  
2008 ◽  
Vol 116 ◽  
pp. 80-82 ◽  
Author(s):  
RENATO DONFRANCESCO ◽  
PAOLA GREGORI ◽  
ANTONELLA VULCANO ◽  
ELISA CANDELORI ◽  
ROBERTO RONCHETTI ◽  
...  
Keyword(s):  
Virus Infection ◽  
Psychiatric Disorders ◽  
Disease Virus ◽  
Borna Disease Virus ◽  
Borna Disease

MRI in psychiatric patients with serum antibodies against Borna disease virus

1989 ◽  
Vol 29 (3) ◽  
pp. 281-282 ◽  
Author(s):  
K. Bechter ◽  
S. Herzog ◽  
R. Schüttler ◽  
R. Rott
Keyword(s):  
Disease Virus ◽  
Psychiatric Patients ◽  
Serum Antibodies ◽  
Borna Disease Virus ◽  
Borna Disease

No association of Borna disease virus with psychiatric disorders among patients in Northern Kyushu, Japan

2000 ◽  
Vol 61 (3) ◽  
pp. 336-340 ◽  
Author(s):  
Katsuro Tsuji ◽  
Koji Toyomasu ◽  
Yoshihiro Imamura ◽  
Hisao Maeda ◽  
Tetsuya Toyoda
Keyword(s):  
Psychiatric Disorders ◽  
Disease Virus ◽  
Borna Disease Virus ◽  
Borna Disease
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