scholarly journals Protection against keratoconjunctivitis shigellosa induced by immunization with outer membrane proteins of Shigella spp

1980 ◽  
Vol 30 (2) ◽  
pp. 321-324
Author(s):  
G Adamus ◽  
M Mulczyk ◽  
D Witkowska ◽  
E Romanowska
Keyword(s):  
Membrane Proteins ◽  
Phase I ◽  
Outer Membrane ◽  
Guinea Pigs ◽  
Shigella Flexneri ◽  
Outer Membrane Proteins ◽  
Passive Immunization ◽  
Active Immunization ◽  
Immune Serum ◽  
Shigella Spp

Active immunization of guinea pigs and rabbits with outer membrane proteins (OMP) isolated from Shigella flexneri 3a and Shigella sonnei phase I protected the animals against keratoconjunctivitis shigellosa induced with the homologous or heterologous strain. Protection was also achieved in rabbits after passive immunization with anti-OMP immune serum. Active immunization with lipopolysaccharide of S. flexneri 3a did not protect rabbits against keratoconjunctivitis shigellosa.

scholarly journals Temperature-regulated expression of outer membrane proteins in Shigella flexneri

Gut Pathogens ◽  
2013 ◽  
Vol 5 (1) ◽  
pp. 38 ◽  
Author(s):  
Hemavathy Harikrishnan ◽  
Asma Ismail ◽  
Kirnpal-Kaur Banga Singh
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Shigella Flexneri ◽  
Outer Membrane Proteins ◽  
Regulated Expression

scholarly journals Characterization of the Outer Membrane Proteome of Leptospira interrogans Expressed during Acute Lethal Infection

2006 ◽  
Vol 75 (2) ◽  
pp. 766-773 ◽  
Author(s):  
Jarlath E. Nally ◽  
Julian P. Whitelegge ◽  
Sara Bassilian ◽  
David R. Blanco ◽  
Michael A. Lovett
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Disease Transmission ◽  
Guinea Pigs ◽  
Outer Membrane Proteins ◽  
Acute Infection ◽  
Hydrophobic Membrane ◽  
Lethal Infection ◽  
Wide Range

ABSTRACT Pathogenic Leptospira species adapt to a wide range of environmental conditions during disease transmission and infection. While the proteome of in vitro cultivated Leptospira has been characterized in several studies to date, relatively little is known of the proteome as expressed by Leptospira during disease processes. Isolates of Leptospira obtained from patients suffering the severe pulmonary form of leptospirosis cause acute lethal infection in guinea pigs and chronic asymptomatic infection in rats. Recent studies have demonstrated that protein and lipopolysaccharide constituents of Leptospira recovered from acutely infected guinea pig tissue differ from that of Leptospira in chronically infected rat tissue and in vitro cultivated Leptospira (J. E. Nally, E. Chow, M. C. Fishbein, D. R. Blanco, and M. A. Lovett, Infect. Immun. 73:3251-3260, 2005). In the current study, the proteome of Leptospira expressed during disease processes was characterized relative to that of in vitro cultivated Leptospira (IVCL) after enrichment for hydrophobic membrane proteins with Triton X-114. Protein samples were separated by two-dimensional gel electrophoresis, and antigens expressed during infection were identified by immunoblotting with monospecific antiserum and convalescent rat serum in addition to mass spectrometry. Results suggest a significant increase in the expression of the outer membrane protein Loa22 during acute infection of guinea pigs relative to other outer membrane proteins, whose expression is generally diminished relative to expression in IVCL. Significant amounts of LipL32 are also expressed by Leptospira during acute infection of guinea pigs.

scholarly journals Passive Immunization to Outer Membrane Proteins MLP and PAL Does Not Protect Mice from Sepsis

2006 ◽  
Vol 12 (9-10) ◽  
pp. 252-258 ◽  
Author(s):  
Catherine H. Valentine ◽  
Judith Hellman ◽  
Laura K. Beasley-Topliffe ◽  
Aranya Bagchi ◽  
H. Shaw Warren
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Passive Immunization

Adaptation of Shigella flexneri to starvation: morphology, outer membrane proteins and lipopolysaccharide changes

2017 ◽  
Vol 10 (12) ◽  
Author(s):  
K. Chourabi ◽  
F. Torrella ◽  
S. Kloula ◽  
J. A. Rodriguez ◽  
I. Trabelsi ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Shigella Flexneri ◽  
Outer Membrane Proteins

Phase I study of a Neisseria meningitidis liposomal vaccine containing purified outer membrane proteins and detoxified lipooligosaccharide

Vaccine ◽  
2012 ◽  
Vol 30 (4) ◽  
pp. 712-721 ◽  
Author(s):  
Wendell D. Zollinger ◽  
Janiine G. Babcock ◽  
Elizabeth E. Moran ◽  
Brenda L. Brandt ◽  
Gary R. Matyas ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Phase I ◽  
Neisseria Meningitidis ◽  
Outer Membrane ◽  
Phase I Study ◽  
Outer Membrane Proteins

Acid stress upregulated outer membrane proteins in clinical isolates ofNeisseria gonorrhoeae, but not most commensalNeisseria

2001 ◽  
Vol 47 (9) ◽  
pp. 871-876 ◽  
Author(s):  
R K Pettit ◽  
T M Whelan ◽  
K S Woo
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Neisseria Gonorrhoeae ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Outer Membrane Proteins ◽  
Acid Stress ◽  
Immune Serum ◽  
Gonococcal Infection ◽  
Membrane Components

Human immune serum recognition of outer membrane components from commensal and pathogenic Neisseria cultured under neutral and acidic conditions was investigated. Acid stress caused no detectable alterations in lipooligosaccharide migration and (or) staining, in outer membrane protein profiles, or in immune serum recognition of outer membrane components from Neisseria mucosa or Neisseria sicca. There was also no difference in the lipoologosaccharide electrophoretic pattern of acid- and neutral-grown Neisseria lactamica, but there were differences in outer membrane protein expression. The outer membrane protein alterations induced by acid stress in N. lactamica were not the same as those seen in isolates from patients with uncomplicated gonococcal infection, pelvic inflammatory disease, and disseminated gonococcal infection. Many differences were detected in the immune serum recognition of outer membrane components from acid- and neutral-cultured N. lactamica and from the clinical isolates of Neisseria gonorrhoeae, and these should be considered in vaccine design.Key words: Neisseria gonorrhoeae, commensal Neisseria, acid stress, outer membrane proteins.

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