scholarly journals Plasmid pT181-linked suppressors of the Staphylococcus aureus pcrA3 chromosomal mutation.

1993 ◽  
Vol 175 (12) ◽  
pp. 3916-3917 ◽  
Author(s):  
S Iordanescu
Keyword(s):  
Staphylococcus Aureus ◽  
Chromosomal Mutation ◽  
Plasmid Pt181

The tet(K) Gene of Plasmid pT181 of Staphylococcus aureus Encodes an Efflux Protein That Contains 14 Transmembrane Helices

Plasmid ◽  
1993 ◽  
Vol 30 (2) ◽  
pp. 163-166 ◽  
Author(s):  
Gordon G. Guay ◽  
Saleem A. Khan ◽  
David M. Rothstein
Keyword(s):  
Staphylococcus Aureus ◽  
Transmembrane Helices ◽  
Plasmid Pt181

scholarly journals Bacillus anthracis and Bacillus cereus PcrA Helicases Can Support DNA Unwinding and In Vitro Rolling-Circle Replication of Plasmid pT181 of Staphylococcus aureus

2004 ◽  
Vol 186 (7) ◽  
pp. 2195-2199 ◽  
Author(s):  
Syam P. Anand ◽  
Poulami Mitra ◽  
Asma Naqvi ◽  
Saleem A. Khan
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Broad Host Range ◽  
Gram Positive ◽  
Rolling Circle ◽  
Initiator Protein ◽  
Gram Positive Bacteria ◽  
Plasmid Pt181

ABSTRACT Replication of rolling-circle replicating (RCR) plasmids in gram-positive bacteria requires the unwinding of initiator protein-nicked plasmid DNA by the PcrA helicase. In this report, we demonstrate that heterologous PcrA helicases from Bacillus anthracis and Bacillus cereus are capable of unwinding Staphylococcus aureus plasmid pT181 from the initiator-generated nick and promoting in vitro replication of the plasmid. These helicases also physically interact with the RepC initiator protein of pT181. The ability of PcrA helicases to unwind noncognate RCR plasmids may contribute to the broad-host-range replication and dissemination of RCR plasmids in gram-positive bacteria.

Sequence and structure of cmp, the replication enhancer of the Staphylococcus aureus plasmid pT181

Gene ◽  
1993 ◽  
Vol 134 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Vitalia Henriquez ◽  
Vladana Milisavljevic ◽  
Jason D. Kahn ◽  
Maria Laura Gennaro
Keyword(s):  
Staphylococcus Aureus ◽  
Plasmid Pt181

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

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