scholarly journals Isolation and partial characterization of Borrelia burgdorferi inner and outer membranes by using isopycnic centrifugation.

1994 ◽  
Vol 176 (24) ◽  
pp. 7447-7455 ◽  
Author(s):  
H A Bledsoe ◽  
J A Carroll ◽  
T R Whelchel ◽  
M A Farmer ◽  
D W Dorward ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Partial Characterization ◽  
Outer Membranes ◽  
Isopycnic Centrifugation

scholarly journals Characterization of outer membranes isolated from Borrelia burgdorferi, the Lyme disease spirochete.

1995 ◽  
Vol 63 (6) ◽  
pp. 2154-2163 ◽  
Author(s):  
J D Radolf ◽  
M S Goldberg ◽  
K Bourell ◽  
S I Baker ◽  
J D Jones ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Membranes

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

Partial characterization of the N-linked oligosaccharides occurring on rat hepatocyte glycoproteins

2010 ◽  
Vol 108 (10) ◽  
pp. 323-329 ◽  
Author(s):  
Marti F. A. Bierhuizen ◽  
Moniek de Wit ◽  
Carin A. R. L. Govers ◽  
Willem van Dijk
Keyword(s):  
Partial Characterization ◽  
Rat Hepatocyte

scholarly journals Localization and Partial Characterization of an Aldehydic Component in Tropocollagen

1966 ◽  
Vol 241 (7) ◽  
pp. 1530-1536
Author(s):  
Marcos Rojkind ◽  
Olga O. Blumenfeld ◽  
Paul M. Gallop
Keyword(s):  
Partial Characterization

scholarly journals Detection and partial characterization of a protein in mouse fibroblasts that binds and inhibits trypsin.

1980 ◽  
Vol 255 (11) ◽  
pp. 5468-5474 ◽  
Author(s):  
R.J. Kirschner ◽  
C.S. Federiuk ◽  
J.P. Ford ◽  
J.A. Shafer
Keyword(s):  
Partial Characterization ◽  
Mouse Fibroblasts
Sign in / Sign up

Export Citation Format

Share Document