mouse fibroblasts
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Author(s):  
Yi-Xuan Zhang ◽  
Li-Ping Liu ◽  
Ming Jin ◽  
Hui Sun ◽  
Han-Lin Zhang ◽  
...  

2021 ◽  
Vol 5 (8) ◽  
pp. 880-896
Author(s):  
Jaeyeaon Cho ◽  
Sangsung Kim ◽  
Hyein Lee ◽  
Woongchan Rah ◽  
Hee Cheol Cho ◽  
...  

2021 ◽  
pp. 112731
Author(s):  
Mulu Geletu ◽  
Hanad Adan ◽  
Maximillian Niit ◽  
Rozanne Arulanandam ◽  
Esther Carefoot ◽  
...  

2021 ◽  
Vol 7 (23) ◽  
pp. eabg5749
Author(s):  
Shao-Hui Pan ◽  
Ning Zhao ◽  
Xiang Feng ◽  
Ying Jie ◽  
Zi-Bing Jin

Reprogramming of somatic cells into desired functional cell types by small molecules has vast potential for developing cell replacement therapy. Here, we developed a stepwise strategy to generate chemically induced neural crest cells (ciNCCs) and chemically induced corneal endothelial cells (ciCECs) from mouse fibroblasts using defined small molecules. The ciNCCs exhibited typical NCC features and could differentiate into ciCECs using another chemical combination in vitro. The resulting ciCECs showed consistent gene expression profiles and self-renewal capacity to those of primary CECs. Notably, these ciCECs could be cultured for as long as 30 passages and still retain the CEC features in defined medium. Transplantation of these ciCECs into an animal model reversed corneal opacity. Our chemical approach for direct reprogramming of mouse fibroblasts into ciNCCs and ciCECs provides an alternative cell source for regeneration of corneal endothelia and other tissues derived from neural crest.


Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2442
Author(s):  
Małgorzata Fischer ◽  
Anna Mertas ◽  
Zenon Paweł Czuba ◽  
Małgorzata Skucha-Nowak

Microinvasive dentistry is based on the treatment of early carious lesions with the use of dental infiltrants. The commercially available Icon dental infiltrant does not contain any bacteriostatic component. An experimental preparation enriched with the missing component was synthesised. The aim of this study was to evaluate the cytotoxicity of the experimental preparation. Mouse fibroblasts of the L-929 lineage were used for the in vitro study. Cell morphology and viability were assessed. In the cytotoxicity analysis, it was shown that the experimental preparation (42.8 ± 10.3) after 24 h at two-fold dilution showed similar cytotoxicity to Icon (42.7 ± 8.8) (p > 0.05), while at four-fold dilution experimental preparation (46.7 ± 3.1), it was less toxic than Icon (34.2 ± 3.1) (p < 0.05). The experimental preparation has the potential to provide an alternative to the Icon commercial preparation. Further research is needed to evaluate the cytotoxicity of the experimental preparation over a longer period of time.


2021 ◽  
pp. 112601
Author(s):  
Mulu Geletu ◽  
Hanad Adan ◽  
Maximillian Niit ◽  
Rozanne Arulanandam ◽  
Esther Carefoot ◽  
...  

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