Influence of Androgen Receptor Antagonist MDV3100 Therapy on Rats With Benign Prostatic Hyperplasia

Purpose: To probe the effect and mechanism of androgen receptor antagonist MDV3100 on benign prostatic hyperplasia (BPH) of ratsMethods: BPH rat model was induced by testosterone propionate. Then antagomir-miR-21-3p or agomir-miR-21-3p was injected into rats before MDV3100 treatment. The prostate index was measured by weighing the wet weight of the rat prostate. The structural morphology of rat prostate was observed after hematoxylin & eosin staining. Immunohistochemistry was applied to evaluate the expression levels of Ki-6 and inflammatory cytokines (interleukin [IL]-6, IL-18, and tumor necrosis factor-α) in rat prostate tissues. Quantitative reverse transcription polymerase chain reaction was utilized for assessment of miR-21-3p expression, and Western blot for the performance of the phosphorylation levels of IKKα and p65.Results: Injection of testosterone propionate caused increased prostate gland hyperplasia, heightened miR-21-3p level, and activated nuclear factor-kappa B (NF-κB) signaling pathway. Additionally, BPH was accompanied by inflammatory response, as evidenced by enhanced expressions of Ki-67 and inflammatory cytokines. MDV3100 exposure ameliorated BPH and suppressed miR-21-3p expression. Overexpression of miR-21-3p intensified BPH and inflammation level, while knockdown of miR-21-3p relieved BPH. The coeffect of miR-21-3p upregulation and MDV3100 subjection led to higher inflammatory response, elevated phosphorylation levels of IKKα and p65 than MDV3100 treatment alone.Conclusions: Androgen receptor antagonist MDV3100 alleviates BPH and inflammatory response through miR-21-3p downregulation and NF-κB signaling pathway blockade.

Histological modifications of the rat prostate following oral administration of silver nanoparticles

Background and aims: Recently, silver nanoparticles (AgNPs) have received much attention for their possible usage in various fields. This study examined the effect of AgNPs on the histopathological changes in the prostate of rats. Methods: In this study, 40 male adult Wistar rats were divided into five equal groups (n=8 in each group). AgNPs were given orally to the four experimental groups at doses of 30, 125, 300, and 700 mg/kg for 28 consecutive days. The control group received deionized water. After performing hematoxylin and eosin (H & E) staining and Masson’s trichrome staining, the histological changes in the prostate of rats were evaluated. Results: Histological evaluation showed that the acinar epithelial height and alveolar folds decreased, but vacuoles in the epithelial cells and accumulation of blood vessel increased in the groups treated with AgNPs at doses of 30 and 125 mg/kg. The collagen content also increased significantly in these groups (30 mg/kg: P=0.03 and 125 mg/kg: P=0.002). Furthermore, the groups treated with AgNPs at doses of 300 and 700 mg/kg showed relative normalization acini and epithelial lining and the amount of their content. Conclusion: According to the results of current study, oral administration of AgNPs for 28 days had effects on prostate, indicating the toxicity of AgNPs.