scholarly journals Determination of Wolbachia Genome Size by Pulsed-Field Gel Electrophoresis

2001 ◽  
Vol 183 (7) ◽  
pp. 2219-2225 ◽  
Author(s):  
Ling V. Sun ◽  
Jeremy M. Foster ◽  
George Tzertzinis ◽  
Midori Ono ◽  
Claudio Bandi ◽  
...  
Keyword(s):  
Genome Size ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Obligate Intracellular ◽  
Before And After ◽  
Wolbachia Genome ◽  
Size Variability ◽  
Obligate Intracellular Bacteria

ABSTRACT Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI,ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number of sites and were used for the determination of the genome sizes ofwMelPop, wMel, and wMelCS (each 1.36 Mb), wRi (1.66 Mb), wBma (1.1 Mb), and wDim (0.95 Mb). The Wolbachia genomes studied were all much smaller than the genomes of free-living bacteria such as Escherichia coli (4.7 Mb), as is typical for obligate intracellular bacteria. There was considerable genome size variability among Wolbachia strains, especially between the more parasitic A group Wolbachia infections of insects and the mutualistic C and D group infections of nematodes. The studies described here found no evidence for extrachromosomal plasmid DNA in any of the strains examined. They also indicated that the Wolbachia genome is circular.

scholarly journals Determination of genome size and a preliminary physical map of an extreme alkaliphile, Micrococcus sp. Y-1, by pulsed-field gel electrophoresis

Microbiology ◽  
1994 ◽  
Vol 140 (9) ◽  
pp. 2247-2250 ◽  
Author(s):  
J. H. Park ◽  
J.-C. Song ◽  
M. H. Kim ◽  
D.-S. Lee ◽  
C.-H. Kim
Keyword(s):  
Genome Size ◽  
Gel Electrophoresis ◽  
Physical Map ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Determination of genome size of four Propionibacterium species by pulsed-field gel electrophoresis

1992 ◽  
Vol 72 (4) ◽  
pp. 421-426 ◽  
Author(s):  
M. Gautier ◽  
N. Mouchel ◽  
A. Rouault ◽  
P. Sanséau
Keyword(s):  
Genome Size ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Determination of the genome size ofEhrlichiaspp., using pulsed field gel electrophoresis

1999 ◽  
Vol 176 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Elena Rydkina ◽  
Veronique Roux ◽  
Didier Raoult
Keyword(s):  
Genome Size ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

Evaluation of Logistic Processing To Reduce Cross-Contamination of Commercial Broiler Carcasses with Campylobacter spp.

2007 ◽  
Vol 70 (11) ◽  
pp. 2549-2554 ◽  
Author(s):  
LAKSHMI-PRASANNA POTTURI-VENKATA ◽  
STEFFEN BACKERT ◽  
SERGIO L. VIEIRA ◽  
OMAR A. OYARZABAL
Keyword(s):  
Gel Electrophoresis ◽  
Food Production ◽  
Pulsed Field Gel Electrophoresis ◽  
Cross Contamination ◽  
Large Problem ◽  
Pulsed Field ◽  
Before And After ◽  
Commercial Broiler ◽  
Simple Logistic ◽  
Campylobacter Spp

Cross-contamination of broiler carcasses with Campylobacter is a large problem in food production. Here, we investigated whether the contamination of broilers carcasses from Campylobacter-negative flocks can be avoided by logistic scheduling during processing. For this purpose, fecal samples were collected from several commercial broiler flocks and enumerated for Campylobacter spp. Based on enumeration results, flocks were categorized as Campylobacter negative or Campylobacter positive. The schedule of processing included the testing of Campylobacter-positive flocks before or after the testing of Campylobacter-negative flocks. During processing, flocks were also sampled for Campylobacter spp. before and after chilling. Campylobacter strains were identified with multiplex PCR and analyzed for relatedness with pulsed-field gel electrophoresis. Our results show that Campylobacter-negative flocks were indeed contaminated with Campylobacter strains originating from previously processed Campylobacter-positive flocks. Campylobacter isolates collected from carcasses originating from different farms processed on the same day showed similar pulsed-field gel electrophoresis patterns, confirming cross-contamination. These findings suggest that a simple logistic processing schedule can preserve the Campylobacter-negative status of broiler carcasses and result in products with enhanced food safety.

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