scholarly journals Genetic Mapping of Mutations Affecting Phosphoglucose Isomerase and Fructose Diphosphatase in Escherichia coli

1967 ◽  
Vol 93 (5) ◽  
pp. 1582-1587 ◽  
Author(s):  
D. G. Fraenkel
Keyword(s):  
Escherichia Coli ◽  
Genetic Mapping ◽  
Phosphoglucose Isomerase

scholarly journals Characterization and in Vivo Cloning of prlC, a Suppressor of Signal Sequence Mutations in Escherichia coli K12

Genetics ◽  
1987 ◽  
Vol 116 (4) ◽  
pp. 513-521
Author(s):  
Nancy J Trun ◽  
Thomas J Silhavy
Keyword(s):  
Escherichia Coli ◽  
Genetic Mapping ◽  
Signal Sequence ◽  
Illegitimate Recombination ◽  
Mutant Phenotype ◽  
E Coli ◽  
Physical Location ◽  
Sequence Deletion ◽  
New Alleles

ABSTRACT The prlC gene of E. coli was originally identified as an allele, prlC1, which suppresses certain signal sequence mutations in the genes for several exported proteins. We have isolated six new alleles of prlC that also confer this phenotype. These mutations can be placed into three classes based on the degree to which they suppress the lamBsignal sequence deletion, lamBs78. Genetic mapping reveals that the physical location of the mutations in prlC correlates with the strength of the suppression, suggesting that different regions of the gene can be altered to yield a suppressor phenotype. We also describe an in vivo cloning procedure using λplacMu9H. The procedure relies on transposition and illegitimate recombination to generate a specialized transducing phage that carries prlC1. This method should be applicable to any gene for which there is a mutant phenotype.

scholarly journals A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.

1989 ◽  
Vol 53 (1) ◽  
pp. 1-24 ◽  
Author(s):  
M Singer ◽  
T A Baker ◽  
G Schnitzler ◽  
S M Deischel ◽  
M Goel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Genetic Mapping

Genetic mapping of the chl C gene of the nitrate reductase a system in Escherichia coli K12

1969 ◽  
Vol 35 (5) ◽  
pp. 659-662 ◽  
Author(s):  
Juan Puig ◽  
Edgard Azoulay ◽  
Francis Pichinoty ◽  
Jacqueline Gendre
Keyword(s):  
Escherichia Coli ◽  
Nitrate Reductase ◽  
Genetic Mapping ◽  
Escherichia Coli K12

Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli

1985 ◽  
Vol 186 (2) ◽  
pp. 283-293 ◽  
Author(s):  
Catherine M. Joyce ◽  
David M. Fujii ◽  
Helaina S. Laks ◽  
Cynthia M. Hughes ◽  
Nigel D.F. Grindley
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Genetic Mapping ◽  
Dna Sequence ◽  
Dna Sequence Analysis

Genetic Mapping of Phenylalanyl-sRNA Synthetase in Escherichia coli

Science ◽  
1967 ◽  
Vol 157 (3784) ◽  
pp. 78-79 ◽  
Author(s):  
A. Bock ◽  
F. C. Neidhardt
Keyword(s):  
Escherichia Coli ◽  
Genetic Mapping
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